Journal
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
Volume 82, Issue 2, Pages 257-265Publisher
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2010.09-0309
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Funding
- Ministry of Research, France [EA3609]
- Institut Pasteur de Lille [IFR142]
- Consejo de Desarrollo Cientifico y Humanistico of the Universidad Central de Venezuela
- French Ministry
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We reported previously that Cryptosporidium parvum was able to induce intestinal tumors in severe combined immunodeficiency (SCID) mice treated with corticoids. To further characterize this Cryptosporidium-induced cell transformation, SCID mice treated with dexamethasone were challenged with C. parvum oocysts, and euthanatized sequentially after infection for histologic examination. Ki-67 was used as a marker of cellular proliferation. Our previous results were confirmed, and it was also found that mice receiving higher inocula (10(6)-10(7)) experienced more severe neoplastic development. Additionally neoplastic changes were observed not only ill the caecum but also in the stomach and duodenum of some animals. Interestingly, SCID mice (6/6) inoculated with 10(5)-10(7) oocysts showed high grade intraepithelial neoplasia or adenomas with high grade dysplasia in the caecum after Day 46 post-infection (PI). Immunohistochemistry for Ki-67 staining indicated the neoplastic process associated to cryptosporidiosis, and evidenced the first immunohistochemical alterations at early stages of the process, even at 3 weeks PI.
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