Journal
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
Volume 83, Issue 3, Pages 549-558Publisher
AMER SOC TROP MED & HYGIENE
DOI: 10.4269/ajtmh.2010.09-0411
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Funding
- Algerian government
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Knowledge of the molecular mechanisms used by Necator americanus larvae to penetrate the human skin and the vasculature would aid the development of effective vaccines against this important pathogen. In this work, the impact of N americanus exsheathing fluid (EF) and excretory/secretory products (ES) on the endothelial barrier was examined using human umbilical vein endothelial cells (HUVEC) Cellular responses were assessed by investigating molecular changes at cell-cell junctions and by determining levels of secreted IL-6, IL-8, and vascular endothelial growth factor (VEGF) in the culture medium It would appear that a repertoire of larval proteases caused a dose-related increase in endothelial permeability as characterized by a decrease in monolayer resistance with increased permeation of tracer-albumin These barrier changes were associated with disruption of junctional vascular endothelial cadherin (VE-cadherin) and F-actin and an Increase in endothelial secretion of IL-6 and IL-8 Our data suggest that larval pi teases play an important role in negotiating the endothelium
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