Article
Immunology
Heather Jennings, Kristin N. N. Carlson, Chris Little, Joshua C. C. Verhagen, Jeevan Nagendran, Yongjun Liu, Bret Verhoven, Weifeng Zeng, Stacey McMorrow, Peter Chlebeck, David P. P. Al-Adra
Summary: The choice of oxygen carrier in normothermic ex vivo liver perfusion (NEVLP) can affect the phenotype and function of liver-resident immune cells, with human pRBC showing stronger activation and Oxyglobin having a less pronounced impact on immune cell activation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Lise N. Tchouta, Fares Alghanem, Alvaro Rojas-Pena, Robert H. Bartlett
Summary: This review discusses the history of ex vivo organ perfusion and identifies the crucial factors necessary for successful perfusion, along with the limitations of current perfusion systems and areas that require further research in preservation science.
Article
Cardiac & Cardiovascular Systems
Lise Tchouta, Daniel Drake, Mark Hoenerhoff, Alvaro Rojas-Pena, Jonathan Haft, Gabe Owens, Robert Bartlett
Summary: This study demonstrates the feasibility of prolonged ex vivo heart perfusion (EVHP) without a paracorporeal animal using continuous plasma exchange (PX), which eliminates the need for an external animal and provides an important step towards clinical application.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2022)
Review
Medicine, General & Internal
Charles W. G. Risbey, Carlo Pulitano
Summary: Liver transplantation is a life-saving procedure for patients with end-stage liver disease, but the shortage of donors prevents many patients from receiving transplants. This paper investigates the clinical progress of ex vivo normothermic machine perfusion (NMP) as an alternative preservation technique for liver transplantation in humans.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Bin Li, Jie Zhang, Chuanyan Shen, Tingting Zong, Cong Zhao, Yumin Zhao, Yunhua Lu, Siyue Sun, Hongli Zhu
Summary: This study demonstrates that within 6 hours of perfusion with pPolyHb, liver physiological and biochemical activities can be effectively maintained in vitro. The optimal addition ratio of pPolyHb was found to be 24%, which significantly reduced the apoptosis rate. This suggests that pPolyHb-based perfusate is feasible for NMP of rat livers and opens up possibilities for further research in this field.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Sarah A. Hosgood, Tegwen R. Elliott, Nina P. Jordan, Michael L. Nicholson
Summary: This study aimed to investigate the levels of free heme during normothermic machine perfusion (NMP) and its effect on kidney function and gene expression. The results showed that older units of red blood cells were associated with higher levels of free heme, and the levels significantly increased during NMP. Transcriptional analysis demonstrated significant upregulation of genes involved in apoptosis, inflammation, and oxidative pathways, but these were not associated with high levels of free heme.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Majid Rezaei, Carlos Ordenana, Brian A. Figueroa, Sayf A. Said, Vahe Fahradyan, Edoardo Dalla Pozza, Lynn M. Orfahli, Michael J. Annunziata, Elizabeth Rohde, Maria Madajka, Francis Papay, Antonio Rampazzo, Bahar Bassiri Gharb
Summary: Ex vivo normothermic perfusion (EVNP) can effectively extend upper extremity preservation time, avoid the adverse effects of hypoxia and cooling, and enable limb quality assessment and reconditioning.
Article
Surgery
Catherine Parmentier, Samrat Ray, Laura I. Mazilescu, Masataka Kawamura, Yuki Noguchi, Emmanuel Nogueira, Sujani Ganesh, Bhranavi Arulratnam, Sangeetha N. Kalimuthu, Markus Selzner, Trevor W. Reichman
Summary: Pancreas transplantation is a curative treatment for complicated diabetes, but there is a shortage of organs. Normothermic ex vivo perfusion of the pancreas may be a solution to expand the donor pool. A study perfused six human pancreases using a previously established method, and all grafts were successfully perfused with minimal damage. This technique shows potential for safe and feasible expansion of the donor pool.
TRANSPLANT INTERNATIONAL
(2023)
Review
Surgery
Amir Fard, Robert Pearson, Rashida Lathan, Patrick B. Mark, Marc J. Clancy
Summary: Ex-vivo normothermic perfusion (EVNP) is an emerging strategy for kidney preservation that allows assessment of viability before transplantation. The optimal composition and duration of perfusate are still uncertain. Current clinical practice commonly uses a perfusate consisting of red blood cells, nutrient solutions, and supplements, but non-blood cell-based perfusates have shown promising results in preclinical studies.
TRANSPLANT INTERNATIONAL
(2022)
Article
Surgery
Rianne Schutter, Veerle A. Lantinga, Tim L. Hamelink, Merel B. F. Pool, Otis C. van Varsseveld, Jan Hendrik Potze, Jan-Luuk Hillebrands, Marius C. van den Heuvel, Rudi A. J. O. Dierckx, Henri G. D. Leuvenink, Cyril Moers, Ronald J. H. Borra
Summary: The study aimed to characterize the regional distribution of perfusate flow during ex vivo normothermic machine perfusion (NMP) using magnetic resonance imaging (MRI), in order to understand how kidney assessment protocols should be designed. Results showed that at the start of NMP, all kidneys were mainly centrally perfused, and it took time for the outer cortex to reach physiological dominant perfusion state. Early functional NMP quality assessment markers may not reflect actual physiology accurately and should be interpreted with caution.
TRANSPLANT INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Ngee-Soon Lau, Mark Ly, Claude Dennis, Andrew Jacques, Marti Cabanes-Creus, Shamus Toomath, Joanna Huang, Nicole Mestrovic, Paul Yousif, Sumon Chanda, Chuanmin Wang, Leszek Lisowski, Ken Liu, James G. Kench, Geoffrey McCaughan, Michael Crawford, Carlo Pulitano
Summary: Long-term machine perfusion of human livers outside the body is an emerging field with tremendous potential for the assessment, recovery, and modification of organs prior to transplantation. Here, the authors report the long-term ex situ perfusion of human livers and demonstrate the ability to split and perfuse these organs using a standardised protocol.
NATURE COMMUNICATIONS
(2023)
Review
Biotechnology & Applied Microbiology
Chuanyan Shen, Hongwei Cheng, Tingting Zong, Hongli Zhu
Summary: The gap between the number of liver transplant patients and available donors is a significant issue in the field of transplantation. Limited access to liver transplantation has led to the use of extended criteria donors to address the growing demand. Preservation before transplantation plays a crucial role in determining patient outcomes, and the use of normothermic machine perfusion shows promise in improving graft viability and assessing viability before transplantation. This review provides an overview of normothermic machine perfusion and its application in liver preservation and pre-transplantation, as well as data from current clinical trials.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Urology & Nephrology
Asel S. Arykbaeva, Dorottya K. de Vries, Jason B. Doppenberg, Marten A. Engelse, Thomas Hankemeier, Amy C. Harms, Leonie G. Wijermars, Alexander F. Schaapherder, Jaap A. Bakker, Rutger J. Ploeg, Ian P. J. Alwayn, Jan H. N. Lindeman
Summary: Normothermic machine perfusion (NMP) is a novel preservation strategy that maintains organs in a metabolically active state, providing superior organ preservation and allowing viability testing before transplantation. However, current perfusion protocols may not fully address the metabolic requirements of marginal donor grafts. Monitoring of metabolic status during NMP is important for optimal outcomes.
KIDNEY INTERNATIONAL
(2021)
Article
Medicine, General & Internal
Aadil Ali, Antti I. Nykanen, Erika Beroncal, Edson Brambate, Andrea Mariscal, Vinicius Michaelsen, Aizhou Wang, Mitsuaki Kawashima, Rafaela V. P. Ribeiro, Yu Zhang, Eddy Fan, Laurent Brochard, Jonathan Yeung, Tom Waddell, Mingyao Liu, Ana C. Andreazza, Shaf Keshavjee, Marcelo Cypel
Summary: This study demonstrates for the first time the feasibility of three-day lung preservation, leading to excellent early post-transplant outcomes. The combination of cold storage and intermittent ex vivo lung perfusion opens up new opportunities in organ transplantation.
Article
Multidisciplinary Sciences
Merel B. F. Pool, Tim L. Hamelink, Harry van Goor, Marius C. van den Heuvel, Henri G. D. Leuvenink, Cyril Moers
Summary: This study compared four different perfusion solutions for renal NMP and found that the composition of perfusate significantly influences measured perfusion and injury parameters. The choice of perfusion solution during NMP directly impacts organ preservation and evaluation. Further research is needed to determine the optimal conditions and develop universal organ assessment criteria.
Article
Surgery
A. D. Barlow, M. O. Hamed, D. H. Mallon, R. J. Brais, F. M. Gribble, M. A. Scott, W. J. Howat, J. A. Bradley, E. M. Bolton, G. J. Pettigrew, S. A. Hosgood, M. L. Nicholson, K. Saeb-Parsy
AMERICAN JOURNAL OF TRANSPLANTATION
(2015)
Article
Surgery
James P. Hunter, Sarah A. Hosgood, Meeta Patel, Peter Furness, Robert D. Sayers, Michael L. Nicholson
ANNALS OF VASCULAR SURGERY
(2015)
Article
Surgery
J. P. Hunter, S. A. Hosgood, A. D. Barlow, M. L. Nicholson
BRITISH JOURNAL OF SURGERY
(2015)
Article
Surgery
S. A. Hosgood, A. D. Barlow, J. P. Hunter, M. L. Nicholson
BRITISH JOURNAL OF SURGERY
(2015)
Article
Surgery
Gwyn Lee, Sarah A. Hosgood, Meeta S. Patel, Michael L. Nicholson
JOURNAL OF SURGICAL RESEARCH
(2015)
Article
Medicine, Research & Experimental
Sarah A. Hosgood, A. D. Barlow, J. Dormer, M. L. Nicholson
JOURNAL OF TRANSLATIONAL MEDICINE
(2015)
Article
Medicine, Research & Experimental
Sarah A. Hosgood, K. Shah, M. Patel, M. L. Nicholson
JOURNAL OF TRANSLATIONAL MEDICINE
(2015)
Article
Medicine, General & Internal
Michael L. Nicholson, Clare J. Pattenden, Adam D. Barlow, James P. Hunter, Gwyn Lee, Sarah A. Hosgood
Letter
Immunology
Michael Nicholson, Sarah Hosgood
Article
Surgery
S. A. Hosgood, K. Saeb-Parsy, M. O. Hamed, M. L. Nicholson
AMERICAN JOURNAL OF TRANSPLANTATION
(2016)
Review
Transplantation
Cissy Yong, Sarah A. Hosgood, Michael L. Nicholson
CURRENT OPINION IN ORGAN TRANSPLANTATION
(2016)
Article
Immunology
Sarah A. Hosgood, Michael L. Nicholson
Article
Medicine, General & Internal
Sarah A. Hosgood, Kourosh Saeb-Parsy, Colin Wilson, Christopher Callaghan, Dave Collett, Michael L. Nicholson
Review
Surgery
Sarah A. Hosgood, Ernest van Heurn, Michael L. Nicholson
TRANSPLANT INTERNATIONAL
(2015)
Review
Transplantation
Ina Jochmans, Michael L. Nicholson, Sarah A. Hosgood
CURRENT OPINION IN ORGAN TRANSPLANTATION
(2017)