Article
Multidisciplinary Sciences
Ye Eun Shim, Youngmin Ko, Jung Pyo Lee, Jin Seok Jeon, Heungman Jun, Jaeseok Yang, Myoung Soo Kim, Seong Jun Lim, Hye Eun Kwon, Joo Hee Jung, Hyunwook Kwon, Young Hoon Kim, Jungbok Lee, Sung Shin
Summary: Anti-thymocyte globulin (ATG) is commonly used as an induction regimen to prevent acute rejection after solid organ transplantation, but the optimal dose for Asian kidney recipients is unclear. A retrospective cohort study in South Korea compared different induction regimens in 4579 adult renal transplantation patients. The study found that high-dose ATG induction (>4.5 mg/kg) showed better graft and patient survival outcomes compared to basiliximab and low-dose ATG induction in Asian kidney transplant patients.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Alin L. Girnita, Lin Wang, Adriana Colovai, Patrick Ahearn, Yorg Azzi, Madhav C. Menon, Marcelo Fernandez-Vina, Howard M. Gebel, E. Steve Woodle, Paolo Cravedi, Jonathan S. Maltzman, Enver Akalin
Summary: This study investigated the changes in anti-HLA antibodies in kidney transplant recipients after COVID-19 and compared them with anti-SARS-CoV-2 antibodies. The results showed that anti-HLA antibodies were mainly IgG, while anti-SARS-CoV-2 antibodies had a high IgA/IgM profile.
Review
Immunology
Edward J. Filippone, John L. Farber
Summary: Studies have shown conflicting results regarding the prognosis of HLA-DSA-negative hAMR cases, with some indicating better outcomes and others demonstrating poor survival. Possible pathophysiological mechanisms for HLA-DSA-negative hAMR include undetected HLA-DSA involvement, non-HLA-DSA involvement, and antibody-independent NK cell activation. More research is needed on measuring non-HLA antibodies, recipient/donor NK cell genotyping, and exploring antibody reduction therapies for this subset of patients.
Article
Immunology
Tareq Hanouneh, Rose Mary Attieh, Emily Craver, Abdullah Jebrini, Mohamed Elrefaei, Tambi Jarmi
Summary: This study investigated the impact of Basiliximab and Alemtuzumab induction therapies on kidney function and de novo donor-specific antibodies (DSA) in blood type A2-to-B kidney transplant recipients. The results showed that Basiliximab was associated with a lower incidence of de novo DSA formation without significant differences in overall renal function compared to Alemtuzumab.
TRANSPLANT IMMUNOLOGY
(2023)
Article
Surgery
Johanna C. Wagner, Emilie Ronin, Patrick Ho, Yani Peng, Qizhi Tang
Summary: CAR Treg therapy targeting donor alloantigens has shown efficacy in prolonging graft survival in vascularized transplant models, offering a potential solution for reducing immunosuppression.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Oncology
Roberto Benelli, Delfina Costa, Laura Salvini, Samuele Tardito, Francesca Tosetti, Federico Villa, Maria Raffaella Zocchi, Alessandro Poggi
Summary: This study generated a novel ADC called Cet-ZA, which can specifically target CRC. Importantly, patient-derived CRC organoids or CRC tumor cell suspensions could induce the expansion of Vδ2 T cells from peripheral blood and tumor infiltrating lymphocytes when primed with Cet-ZA, and Cet-ZA can promote Vδ2 T cell-mediated killing of CRC organoids.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Medicine, General & Internal
Claudia M. M. Munoz-Herrera, Juan Francisco Gutierrez-Bautista, Miguel Angel Lopez-Nevot
Summary: This study evaluated the association between complement-fixing dnDSA antibodies and graft loss, as well as the possible association between non-complement-fixing antibodies and transplanted organ survival in kidney transplant recipients. The presence of C1q-binding dnDSA was significantly associated with rejection frequency and renal graft loss. These findings suggest that classifying dnDSA based on their ability to activate the complement and detecting complement-binding capacity could serve as prognostic markers for predicting AMR outcome and graft survival in kidney transplant patients who develop dnDSA.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Hematology
Thijs L. J. van Osch, Tamas Pongracz, Dionne M. Geerdes, Juk Yee Mok, Wim J. E. van Esch, Jan Voorberg, Rick Kapur, Leendert Porcelijn, Jean-Louis H. Kerkhoffs, Pieter F. van Der Meer, C. Ellen van Der Schoot, Masja de Haas, Manfred Wuhrer, Gestur Vidarsson
Summary: The Fc glycosylation profile of anti-HLA class I antibodies formed after platelet transfusion varies between patients, and could potentially explain the variation in clinical severity.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Surgery
Michael Y. Shino, Qiuheng Zhang, Ning Li, Ariss Derhovanessian, Allison Ramsey, Rajan Saggar, Ian N. Britton, Olawale O. Amubieya, Shahrzad M. Lari, Michelle Hickey, Elaine F. Reed, Paul W. Noble, Barry R. Stripp, Gregory A. Fishbein, Joseph P. Lynch, Abbas Ardehali, David M. Sayah, S. Sam Weigt, John A. Belperio
Summary: This study found that after lung transplantation, the presence of donor-specific HLA antibodies (DSAs) was associated with an increased risk of CLAD development, while non-donor specific anti-HLA antibodies (non-DSAs) generally did not increase CLAD risk but could do so when combined with high BAL CXCL9 levels. The study also highlighted the potential utility of BAL CXCL9 measurement as a biomarker for risk stratifying HLA antibodies in future CLAD management.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Pharmacology & Pharmacy
Stefania Cheli, Diego Savino, Francesca Penagini, Gianvincenzo Zuccotti, Giovanna Zuin, Emilio Clementi, Dario Cattaneo
Summary: Therapeutic drug monitoring (TDM) is important for optimizing the use of anti-TNF-alpha inhibitors in inflammatory bowel disease patients. A recent study evaluated the performance of an automated ELISA-based immunoassay and a lateral flow assay for quantification of drug levels, showing good agreement for adalimumab but weak correlation for infliximab. The findings suggest that the two assays are not interchangeable, emphasizing the need for consistent use in patient follow-up.
Article
Immunology
Tsukasa Nakamura, Takayuki Shirouzu, Ryusuke Sugimoto, Shumpei Harada, Mikiko Yoshikawa, Shuji Nobori, Hidetaka Ushigome, Shintaro Kawai
Summary: This study suggests that the presence of graft intragraft DSA (g-DSA) and intragraft C3d-binding reaction had a negative impact on liver allografts, while serum DSA (s-DSA) did not have a significant effect.
TRANSPLANTATION PROCEEDINGS
(2022)
Article
Medical Laboratory Technology
Hyun Ji Lee, Kyung-Hwa Shin, Il Young Kim, Byung Hyun Choi, Hyung-Hoi Kim
Summary: This study found that anti-ETAR antibodies are associated with AMR, and patients with both anti-ETAR antibodies and de novo HLA DSAs were at a high risk of AMR.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Martin Reichert, Srebrena Atanasova, Kathrin Petri, Marian Kampschulte, Baktybek Kojonazarov, Gabriele Fuchs-Moll, Gabriele A. Krombach, Winfried Padberg, Veronika Grau
Summary: Alloreactive and autoreactive antibodies have been associated with chronic lung allograft dysfunction, with lipopolysaccharide-induced infiltration of B cells and accumulation of immunoglobulins potentially contributing to the pathogenesis of this condition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Surgery
Joshua A. Hill, Erika S. Kiem, Atif Bhatti, Winnie Liu, Jacob Keane-Candib, Kristin S. Fitzpatrick, Jim Boonyaratanakornkit, Rebecca A. Gardner, Damian J. Green, David G. Maloney, Cameron J. Turtle, Jodi M. Smith, Idoia Gimferrer, Christopher D. Blosser, Shaun W. Jackson
Summary: The presence of anti-HLA antibodies hinders successful organ transplantation. B cell-depleting treatments have limited effectiveness in reducing these antibodies. This study suggests that long-lived plasma cells, not effectively targeted by B cell depletion, are the primary source of anti-HLA antibodies. Plasma cell-targeted therapies may be more effective in managing HLA antibodies and improving access to organ transplantation.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Immunology
Elisabet Van Loon, Baptiste Lamarthee, Thomas Barba, Sandra Claes, Maarten Coemans, Henriette de Loor, Marie-Paule Emonds, Priyanka Koshy, Dirk Kuypers, Paul Proost, Aleksandar Senev, Ben Sprangers, Claire Tinel, Olivier Thaunat, Amaryllis H. Van Craenenbroeck, Dominique Schols, Maarten Naesens
Summary: Through multiplex assay, we found that kidney transplant patients with donor-specific anti-human leukocyte antigen antibodies and histological rejection have increased levels of pro-inflammatory cytokines in blood. Even in patients without histological rejection, elevated pro-inflammatory cytokine levels can be observed. These findings challenge the concept of histology as the gold standard for identifying ongoing allo-immune activation after transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Chalmers Chau, Paolo Actis, Eric Hewitt
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Article
Chemistry, Multidisciplinary
Chalmers C. Chau, Sheena E. Radford, Eric W. Hewitt, Paolo Actis
Article
Medicine, General & Internal
Sofia Christakoudi, Manohursingh Runglall, Paula Mobillo, Irene Rebollo-Mesa, Tjir-Li Tsui, Estefania Nova-Lamperti, Catharine Taube, Sonia Norris, Yogesh Kamra, Rachel Hilton, Titus Augustine, Sunil Bhandari, Richard Baker, David Berglund, Sue Carr, David Game, Sian Griffi, Philip A. Kalra, Robert Lewis, Patrick B. Mark, Stephen D. Marks, Iain MacPhee, William McKane, Markus G. Mohaupt, Estela Paz-Artal, Sui Phin Kon, Daniel Seron, Manish D. Sinha, Beatriz Tucker, Ondrej Viklicky, Daniel Stahl, Robert I. Lechler, Graham M. Lord, Maria P. Hernandez-Fuentes
Article
Rheumatology
Guillermo Barturen, Sepideh Babaei, Francesc Catala-Moll, Manuel Martinez-Bueno, Zuzanna Makowska, Jordi Martorell-Marugan, Pedro Carmona-Saez, Daniel Toro-Dominguez, Elena Carnero-Montoro, Maria Teruel, Martin Kerick, Marialbert Acosta-Herrera, Lucas Le Lann, Christophe Jamin, Javier Rodriguez-Ubreva, Antonio Garcia-Gomez, Jorge Kageyama, Anne Buttgereit, Sikander Hayat, Joerg Mueller, Ralf Lesche, Maria Hernandez-Fuentes, Maria Juarez, Tania Rowley, Ian White, Concepcion Maranon, Tania Gomes Anjos, Nieves Varela, Rocio Aguilar-Quesada, Francisco Javier Garrancho, Antonio Lopez-Berrio, Manuel Rodriguez Maresca, Hector Navarro-Linares, Isabel Almeida, Nancy Azevedo, Mariana Brandao, Ana Campar, Raquel Faria, Fatima Farinha, Antonio Marinho, Esmeralda Neves, Ana Tavares, Carlos Vasconcelos, Elena Trombetta, Gaia Montanelli, Barbara Vigone, Damiana Alvarez-Errico, Tianlu Li, Divya Thiagaran, Ricardo Blanco Alonso, Alfonso Corrales Martinez, Fernanda Genre, Raquel Lopez Mejias, Miguel A. Gonzalez-Gay, Sara Remuzgo, Begona Ubilla Garcia, Ricard Cervera, Gerard Espinosa, Ignasi Rodriguez-Pinto, Ellen De Langhe, Jonathan Cremer, Rik Lories, Doreen Belz, Nicolas Hunzelmann, Niklas Baerlecken, Katja Kniesch, Torsten Witte, Michaela Lehner, Georg Stummvoll, Michael Zauner, Maria Angeles Aguirre-Zamorano, Nuria Barbarroja, Maria Carmen Castro-Villegas, Eduardo Collantes-Estevez, Enrique de Ramon, Isabel Diaz Quintero, Alejandro Escudero-Contreras, Maria Concepcion Fernandez Roldan, Yolanda Jimenez Gomez, Inmaculada Jimenez Moleon, Rosario Lopez-Pedrera, Rafaela Ortega-Castro, Norberto Ortego, Enrique Raya, Carolina Artusi, Maria Gerosa, Pier Luigi Meroni, Tommaso Schioppo, Aurelie De Groof, Julie Ducreux, Bernard Lauwerys, Anne-Lise Maudoux, Divi Cornec, Valerie Devauchelle-Pensec, Sandrine Jousse-Joulin, Pierre-Emmanuel Jouve, Benedicte Rouviere, Alain Saraux, Quentin Simon, Montserrat Alvarez, Carlo Chizzolini, Aleksandra Dufour, Donatienne Wynar, Attila Balog, Marta Bocskai, Magdolna Deak, Sonja Dulic, Gabriella Kadar, Laszlo Kovacs, Qingyu Cheng, Velia Gerl, Falk Hiepe, Laleh Khodadadi, Silvia Thiel, Emanuele de Rinaldis, Sambasiva Rao, Robert J. Benschop, Chris Chamberlain, Ernst R. Dow, Yiannis Ioannou, Laurence Laigle, Jacqueline Marovac, Jerome Wojcik, Yves Renaudineau, Maria Orietta Borghi, Johan Frostegard, Javier Martin, Lorenzo Beretta, Esteban Ballestar, Fiona McDonald, Jacques-Olivier Pers, Marta E. Alarcon-Riquelme
Summary: The study identified and validated four clusters, one healthy and three pathological, for systemic autoimmune diseases based on molecular patterns. Patients remained stable within their pathological clusters over time, with distinct molecular signatures characterizing each individual. This reclassification has significant implications for future clinical trials and understanding nonresponse to therapy, indicating a paradigm shift in the understanding of systemic autoimmune diseases.
ARTHRITIS & RHEUMATOLOGY
(2021)
Review
Neurosciences
Madeleine R. Brown, Sheena E. Radford, Eric W. Hewitt
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2020)
Article
Cell Biology
Aravind Cherukuri, Alan D. Salama, Rajil Mehta, Kanishka Mohib, Leting Zheng, Ciara Magee, Mark Harber, Hans Stauss, Richard J. Baker, Amit Tevar, Douglas Landsittel, Fadi G. Lakkis, Sundaram Hariharan, David M. Rothstein
Summary: The study found that the T1B IL-10/TNF-α ratio 3 months after transplantation is an effective biomarker for predicting renal allograft rejection and patient clinical course, helping to identify high-risk patients early, guide treatment, and improve graft survival.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Review
Chemistry, Physical
Chalmers C. Chau, Eric W. Hewitt, Paolo Actis
Summary: The development of nanoscale probes has allowed for unprecedented resolution in studying single molecules and single cells, with evidence suggesting that crowded intracellular environments can improve signal-to-noise ratios in nano-electrochemical measurements within cells. This article discusses the concept of macromolecular crowding and its implications in single-entity electrochemistry.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Immunology
Ramon M. Rodriguez, Maria P. Hernandez-Fuentes, Viviana Corte-Iglesias, Maria Laura Saiz, Juan Jose Lozano, Ana R. Cortazar, Isabel Mendizabal, Maria Luisa Suarez-Fernandez, Eliecer Coto, Antonio Lopez-Vazquez, Carmen Diaz-Corte, Ana M. Aransay, Carlos Lopez-Larrea, Beatriz Suarez-Alvarez
Summary: Genome-wide DNA methylation analysis in kidney transplant recipients revealed distinct methylation signatures associated with operational tolerance and chronic rejection. Tolerance was linked to demethylation in immune function genes, while rejection was associated with intracellular signaling and ubiquitination pathways. Analysis of methylation patterns in tolerant patients showed similarities with those receiving low doses of steroids, suggesting a potential epigenetic mechanism for tolerance.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Urology & Nephrology
Johnny Bou Saba, Aravind Cherukuri
Summary: Burton et al. present a convincing method for identifying and enumerating human leukocyte antigen-specific B cells and subsets using biotinylated human leukocyte antigen proteins. They find that antigen-specific B cells are more frequent in sensitized kidney transplant recipients compared to healthy volunteers. Additionally, they use an indirect antigen-specific enzyme-linked immunosorbent spot assay to uncover the complexity of B- and T-cell interactions and the influence of regulatory T cells in vitro.
KIDNEY INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Chalmers Chau, Fabio Marcuccio, Dimitrios Soulias, Martin Andrew Edwards, Andrew Tuplin, Sheena E. Radford, Eric Hewitt, Paolo Actis
Summary: This study presents a polymer-electrolyte solid-state nanopore system for analyzing biomolecular conformation under physiological conditions. By using specific salts, the system's performance is enhanced, allowing for the analysis of Chikungunya virus RNA conformation.
Article
Nanoscience & Nanotechnology
Fabio Marcuccio, Dimitrios Soulias, Chalmers C. C. Chau, Sheena E. Radford, Eric Hewitt, Paolo Actis, Martin Andrew Edwards
Summary: In this study, the addition of poly(ethylene) glycol (PEG) to the external solution is shown to enhance the detection of biomolecules using solid-state nanopores. The researchers demonstrate that this addition creates an imbalance in the transport properties of cations and anions, which significantly affects the current response of the nanopore. The findings suggest that tuning the diffusion coefficients of ions could enhance the sensitivity of nanopore sensing.
ACS NANOSCIENCE AU
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
B. Saez-Gimenez, A. Mendoza-Valderrey, M. Hernandez-Fuentes, R. Escobar, C. Berastegui Garcia, A. Sole, S. Fernandez-Rozas, M. de la Torre, R. Laporta, J. Redel, S. Gomez-Olles, A. Roman
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2021)
Correction
Medicine, General & Internal
Birgit Sawitzki, Paul N. Harden, Petra Reinke, Aurelie Moreau, James A. Hutchinson, David S. Game, Qizhi Tang, Eva C. Guinan, Manuela Battaglia, William J. Burlingham, Ian S. D. Roberts, Mathias Streitz, Regis Josien, Carsten A. Boeger, Cristiano Scotta, James F. Markmann, Joanna L. Hester, Karsten Juerchott, Cecile Braudeau, Ben James, Laura Contreras-Ruiz, Jeroen B. van der Net, Tobias Bergler, Rossana Caldara, William Petchey, Matthias Edinger, Nathalie Dupas, Michael Kapinsky, Ingrid Mutzbauer, Natalie M. Otto, Robert Oellinger, Maria P. Hernandez-Fuentes, Fadi Issa, Norbert Ahrens, Christoph Meyenberg, Sandra Karitzky, Ulrich Kunzendorf, Stuart J. Knechtle, Josep Grinyo, Peter J. Morris, Leslie Brent, Andrew Bushell, Laurence A. Turka, Jeffrey A. Bluestone, Robert I. Lechler, Hans J. Schlitt, Stephan Schlickeiser, Maria C. Cuturi, Stephan Schlickeiser, Peter J. Friend, Tewfik Miloud, Alexander Scheffold, Antonio Secchi, Kerry Crisalli, Sang-Mo Kang, Rachel Hilton, Bernhard Banas, Gilles Blancho, Hans-Dieter Volk, Giovanna Lombardi, Kathryn J. Wood, Edward K. Geissler
Article
Medicine, General & Internal
Birgit Sawitzki, Paul N. Harden, Petra Reinke, Aurelie Moreau, James A. Hutchinson, David S. Game, Qizhi Tang, Eva C. Guinan, Manuela Battaglia, William J. Burlingham, Ian S. D. Roberts, Mathias Streitz, Regis Josien, Carsten A. Boeger, Cristiano Scotta, James F. Markmann, Joanna L. Hester, Karsten Juerchott, Cecile Braudeau, Ben James, Laura Contreras-Ruiz, Jeroen B. van der Net, Tobias Bergler, Rossana Caldara, William Petchey, Matthias Edinger, Nathalie Dupas, Michael Kapinsky, Ingrid Mutzbauer, Natalie M. Otto, Robert Oellinger, Maria P. Hernandez-Fuentes, Fadi Issa, Norbert Ahrens, Christoph Meyenberg, Sandra Karitzky, Ulrich Kunzendorf, Stuart J. Knechtle, Josep Grinyo, Peter J. Morris, Leslie Brent, Andrew Bushell, Laurence A. Turka, Jeffrey A. Bluestone, Robert I. Lechler, Hans J. Schlitt, Maria C. Cuturi, Stephan Schlickeiser, Peter J. Friend, Tewfik Miloud, Alexander Scheffold, Antonio Secchi, Kerry Crisalli, Sang-Mo Kang, Rachel Hilton, Bernhard Banas, Gilles Blancho, Hans-Dieter Volk, Giovanna Lombardi, Kathryn J. Wood, Edward K. Geissler
Meeting Abstract
Cardiac & Cardiovascular Systems
B. Saez-Gimenez, A. Mendoza-Valderrey, M. Hernandez-Fuentes, R. Escobar, B. Cristina, A. Sole, F. Zurbano, M. De la Torre, R. Laporta, R. Javier, G. Susana, A. Roman
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2020)