Review
Biochemistry & Molecular Biology
Serena Barachini, Letizia Biso, Shivakumar Kolachalam, Lacopo Petrini, Roberto Maggio, Marco Scarselli, Biancamaria Longoni
Summary: Pancreatic islet transplantation is a treatment for Type 1 diabetic patients, and mesenchymal stem cells (MSCs) have shown potential in controlling graft rejection. MSCs can interact with immune system cells through direct contact or their secretome. This review discusses the role of MSCs in controlling the immune system and promoting repair in pancreatic islet transplantation, as well as challenges such as oxidative stress and impaired vasculogenesis. Preconditioning MSCs with hypoxia exposure or using stem cells with angiogenic potential in organoids may help improve transplant outcomes.
Article
Biotechnology & Applied Microbiology
Xiaoyu Yu, Pu Zhang, Yi He, Emily Lin, Huiwang Ai, Melur K. Ramasubramanian, Yong Wang, Yuan Xing, Jose Oberholzer
Summary: Islet beta-cell viability, function, and mass are key attributes for the efficacy of islet transplantation in type 1 diabetes patients. A Smartphone-Fluidic Digital Imaging Analysis (SFDIA) System was developed to quantitatively assess islet mass, showing more accurate and consistent results compared to manual counting methods.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Maximilian M. Menger, Lisa Nalbach, Leticia P. Roma, Matthias W. Laschke, Michael D. Menger, Emmanuel Ampofo
Summary: Exposing isolated pancreatic islets to EPO does not affect viability and ROS production, but increases CD31-positive cells and insulin/VEGF-A gene expression. EPO-cultivated islets exhibit accelerated revascularization post-transplantation compared to controls, suggesting EPO as a promising approach for clinical islet transplantation.
ACTA DIABETOLOGICA
(2021)
Review
Immunology
Erinn Zixuan Sim, Nobuaki Shiraki, Shoen Kume
Summary: Recent advancements in differentiating PSCs into pancreatic islet cells have led to the discovery of novel signaling pathways and molecules, with potential therapeutic applications demonstrated through cell transplantation studies. Single-cell transcriptomics and metabolomics have provided valuable insights into endocrine cell growth and pancreatic development.
INFLAMMATION AND REGENERATION
(2021)
Article
Multidisciplinary Sciences
Nikola Dolezalova, Anja Gruszczyk, Kerry Barkan, John A. Gamble, Sam Galvin, Till Moreth, Kevin O'Holleran, Krishnaa T. Mahbubani, Jackie A. Higgins, Fiona M. Gribble, Frank Reimann, Jakub Surmacki, Simon Andrews, John J. Casey, Francesco Pampaloni, Michael P. Murphy, Graham Ladds, Nigel K. H. Slater, Kourosh Saeb-Parsy
Summary: The study identified a method to improve cryosurvival rate of pancreatic islets by accelerating equilibration of small molecules with aqueous solutions through heating, and developed a novel cryopreservation protocol combining trehalose and DMSO to enhance cell cryosurvival rate and function.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
A. M. James Shapiro, David Thompson, Thomas W. Donner, Melena D. Bellin, Willa Hsueh, Jeremy Pettus, Jon Wilensky, Mark Daniels, Richard M. Wang, Eugene P. Brandon, Manasi S. Jaiman, Evert J. Kroon, Kevin A. D'Amour, Howard L. Foyt
Summary: Preliminary data from a phase 1/2 study show that pluripotent stem cell-derived pancreatic endoderm cells engrafted in type 1 diabetes patients can develop into functional islet cells releasing insulin in a regulated manner. Although engraftment and insulin expression were observed in some subjects, potential adverse events related to surgical implantation or immunosuppression remain a concern.
CELL REPORTS MEDICINE
(2021)
Article
Oncology
Tomohiro Tanaka, Charles F. Lynch, Kelly J. Yu, Bozena M. Morawski, Mei-Chin Hsieh, Georgetta Alverson, April A. Austin, Yun Zeng, Eric A. Engels
Summary: The incidence of pancreatic cancer is higher in solid organ transplant recipients, particularly in liver transplant recipients. Compared with the general population, pancreatic cancer is more commonly diagnosed in transplant recipients, especially in cases arising in the head of the pancreas and those diagnosed at localized stage. Risk factors for pancreatic cancer in transplant recipients are consistent with those in the general population, including male sex, older age, non-O blood type, and history of diabetes.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Review
Nutrition & Dietetics
Agnieszka Mizerska, Marek Durlik, Karolina Kedzierska-Kapuza
Summary: The significance of nutritional status and support in simultaneous pancreatic and kidney transplantation is still poorly investigated. This article gathered evidence and information about the subject, found various tools for predicting patient outcomes, and emphasized the necessity of nutritional screening and adequate therapy while awaiting transplantation.
Article
Biochemistry & Molecular Biology
Daniel Norman, Carl Johan Drott, Per-Ola Carlsson, Daniel Espes
Summary: Irisin is a myokine involved in glucose homeostasis that is found in human and rat islets. Its secretion is regulated by glucose, but it does not affect insulin and glucagon secretion. However, Irisin does reduce islet blood flow without affecting insulin secretion.
Article
Pharmacology & Pharmacy
Kateryna Polishevska, Sandra Kelly, Purushothaman Kuppan, Karen L. Seeberger, Saloni Aggarwal, Joy Paramor, Larry D. Unsworth, Hubert M. Tse, Gregory S. Korbutt, Andrew R. Pepper
Summary: Beta cell replacement therapies can restore glycemic control in type 1 diabetes, but the need for lifelong immunosuppression hinders its application. Using a conformal coating of poly(N-vinylpyrrolidone) (PVPON) and tannic acid (TA) (PVPON/TA), researchers tested if islet function could be preserved while protecting against rejection. The coating demonstrated the ability to maintain in vitro and in vivo function of murine and human islets, and also reduced post-transplant immune responses.
Article
Cell & Tissue Engineering
Rei Kuwabara, Shuxian Hu, Alexandra M. Smink, Gorka Orive, Jonathan R. T. Lakey, Paul de Vos
Summary: Islet transplantation holds promise for treating insulin-dependent diabetes, but challenges remain due to the lack of a consistent source of insulin-producing cells and the need for lifelong administration of immunosuppressive drugs. Immunoisolation using polymeric membranes shows safety and feasibility in controlling glucose homeostasis, but long-term graft survival is not yet achieved. Combining immunoisolation with immunomodulation strategies may enhance the longevity of islet grafts, but further research is needed to fully understand the reasons for final graft failure and improve outcomes.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Biotechnology & Applied Microbiology
Patricia Wu Jin, Nassim Rousset, Andreas Hierlemann, Patrick M. Misun
Summary: A new generation microfluidic hanging-drop-based islet perifusion platform was developed to study dynamic insulin secretion from individual pancreatic islets with high temporal resolution. The platform was redesigned to increase experimental throughput and simplify operation, achieving continuous insulin release observation with reproducible results. This device will be valuable in islet and diabetes research for studying alterations in insulin secretion dynamics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Surgery
Nina L. Owen-Simon, Geoffrey K. Dube, Pedro Rodrigo Sandoval, Lloyd E. Ratner, Kasi McCune
Summary: To address long waitlist times and increase pancreas transplantation, our center has implemented a protocol for long-distance importation of pancreata. After comparing locally procured grafts and imported grafts, we found that imported grafts were more likely to come from pediatric donors and donors weighing less than 30 kg, but had a longer cold ischemic time. The study concluded that expanding the criteria for accepting imported pancreata could increase the number of transplants.
CLINICAL TRANSPLANTATION
(2023)
Review
Endocrinology & Metabolism
Neil Tanday, Andrei I. Tarasov, R. Charlotte Moffett, Peter R. Flatt, Nigel Irwin
Summary: The development of pancreatic islet endocrine cells is tightly regulated, and small changes in the environment can lead to the generation of different cell types with distinct hormones. Cell differentiation is driven by transcription factors, which are also critical for maintaining mature islet cell phenotype. Prolonged metabolic stress can alter the transcription factor set of insulin-secreting beta cells, leading to loss of beta-cell identity through de- or transdifferentiation. Approved and experimental antidiabetic agents have been associated with preventing beta-cell dedifferentiation or promoting the transdifferentiation of non-beta cells towards an insulin-positive beta-cell-like phenotype. Understanding islet cell plasticity is important for preventing beta-cell decline in diabetes.
DIABETES OBESITY & METABOLISM
(2023)
Article
Endocrinology & Metabolism
Hiroyuki Kato, Tatsuaki Miwa, Janine Quijano, Leonard Medrano, Jose Ortiz, Akiko Desantis, Keiko Omori, Aya Wada, Kentaro Tatsukoshi, Fouad Kandeel, Yoko Mullen, Hsun Teresa Ku, Hirotake Komatsu
Summary: The study introduced a microwell platform optimized for human islets to prevent fusion, maintaining their viability and mass in long-term cultures.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mejdi Najjar, Vita Manzoli, Maria Abreu, Chiara Villa, Mikael M. Martino, R. Damaris Molano, Yvan Torrente, Antonello Pileggi, Luca Inverardi, Camillo Ricordi, Jeffrey A. Hubbell, Alice A. Tomei
BIOTECHNOLOGY AND BIOENGINEERING
(2015)
Article
Endocrinology & Metabolism
Dagmar Klein, Silvia Alvarez-Cubela, Giacomo Lanzoni, Nancy Vargas, Kamalaveni R. Prabakar, Maria Boulina, Camillo Ricordi, Luca Inverardi, Ricardo L. Pastori, Juan Dominguez-Bendala
Article
Endocrinology & Metabolism
Jinquan Cai, Zhixian Wu, Xiumin Xu, Lianming Liao, Jin Chen, Lianghu Huang, Weizhen Wu, Fang Luo, Chenguang Wu, Alberto Pugliese, Antonello Pileggi, Camillo Ricordi, Jianming Tan
Article
Endocrinology & Metabolism
Omaima M. Sabek, Satoru Ken Nishimoto, Daniel Fraga, Neelam Tejpal, Camillo Ricordi, A. O. Gaber
Review
Biotechnology & Applied Microbiology
Alice Anna Tomeit, Chiara Villa, Camillo Ricordi
EXPERT OPINION ON BIOLOGICAL THERAPY
(2015)
Article
Medicine, Research & Experimental
G. Zhou, J. Yu, A. Wang, S. -H. Liu, J. Sinnett-Smith, J. Wu, R. Sanchez, J. Nemunaitis, C. Ricordi, E. Rozengurt, F. C. Brunicardi
CURRENT MOLECULAR MEDICINE
(2016)
Article
Endocrinology & Metabolism
Dora M. Berman, Damaris Molano, Carmen Fotino, Ulisse Ulissi, Jennifer Gimeno, Armando J. Mendez, Norman M. Kenyon, Norma S. Kenyon, David M. Andrews, Camillo Ricordi, Antonello Pileggi
Article
Endocrinology & Metabolism
Bernhard J. Hering, William R. Clarke, Nancy D. Bridges, Thomas L. Eggerman, Rodolfo Alejandro, Melena D. Bellin, Kathryn Chaloner, Christine W. Czarniecki, Julia S. Goldstein, Lawrence G. Hunsicker, Dixon B. Kaufman, Olle Korsgren, Christian P. Larsen, Xunrong Luo, James F. Markmann, Ali Naji, Jose Oberholzer, Andrew M. Posselt, Michael R. Rickels, Camillo Ricordi, Mark A. Robien, Peter A. Senior, A. M. James Shapiro, Peter G. Stock, Nicole A. Turgeon
Review
Immunology
James F. Markmann, Stephen T. Bartlett, Paul Johnson, Olle Korsgren, Bernhard J. Hering, David Scharp, Thomas W. H. Kay, Jonathan Bromberg, Jon S. Odorico, Gordon C. Weir, Nancy Bridges, Raja Kandaswamy, Peter Stock, Peter Friend, Mitsukazu Gotoh, David K. C. Cooper, Chung-Gyu Park, Philip J. O'Connell, Cherie Stabler, Shinichi Matsumoto, Barbara Ludwig, Pratik Choudhary, Boris Khovatchev, Michael R. Rickels, Megan Sykes, Kathryn Wood, Kristy Kraemer, Albert Hwa, Edward Stanley, Camillo Ricordi, Mark Zimmerman, Julia Greenstein, Eduard Montanya, Timo Otonkoski
Article
Immunology
Stephen T. Bartlett, James F. Markmann, Paul Johnson, Olle Korsgren, Bernhard J. Hering, David Scharp, Thomas W. H. Kay, Jonathan Bromberg, Jon S. Odorico, Gordon C. Weir, Nancy Bridges, Raja Kandaswamy, Peter Stock, Peter Friend, Mitsukazu Gotoh, David K. C. Cooper, Chung-Gyu Park, Phillip O'Connell, Cherie Stabler, Shinichi Matsumoto, Barbara Ludwig, Pratik Choudhary, Boris Kovatchev, Michael R. Rickels, Megan Sykes, Kathryn Wood, Kristy Kraemer, Albert Hwa, Edward Stanley, Camillo Ricordi, Mark Zimmerman, Julia Greenstein, Eduard Montanya, Timo Otonkoski
Letter
Surgery
Piotr Witkowski, Rolf N. Barth, Anthony Japour, Gail Javitt, Jordan S. Pyda, Piotr J. Bachul, Eryk Nowicki, Camillo Ricordi
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Biochemistry & Molecular Biology
Marta De Angelis, David Della-Morte, Gabriele Buttinelli, Angela Di Martino, Francesca Pacifici, Paola Checconi, Luigina Ambrosio, Paola Stefanelli, Anna Teresa Palamara, Enrico Garaci, Camillo Ricordi, Lucia Nencioni
Summary: Studies have shown that the A5+ mixture is more effective in inhibiting influenza virus and SARS-CoV-2 infections, reducing viral protein expression and viral release. Additionally, A5+ treatment significantly decreases IL-6 cytokine production in influenza virus-infected cells.
Article
Endocrinology & Metabolism
Marco Infante, Camillo Ricordi
Summary: Diabetes of the exocrine pancreas (DEP) refers to different types of diabetes resulting from disorders of the exocrine pancreas. Post-total pancreatectomy diabetes mellitus (PTPDM) is a unique form of DEP with distinct pathophysiological and clinical features.
EXPERT REVIEW OF ENDOCRINOLOGY & METABOLISM
(2023)
Article
Immunology
Marco Infante, Laura Vitiello, Andrea Fabbri, Camillo Ricordi, Nathalia Padilla, Francesca Pacifici, Pasquale Di Perna, Marina Passeri, David Della-Morte, Massimiliano Caprio, Luigi Uccioli
Summary: We describe a case of a 22-year-old Italian man with new-onset type 1 diabetes who experienced an unusually prolonged clinical remission phase (31 months). The patient was treated with calcifediol and low-dose insulin, resulting in the maintenance of residual beta-cell function and clinical remission. This may be attributed to the immunoregulatory properties of calcifediol.
Article
Pharmacology & Pharmacy
R. Codella, L. Luzi, L. Inverardi, C. Ricordi
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2015)