Journal
AMERICAN JOURNAL OF SURGERY
Volume 204, Issue 5, Pages 598-601Publisher
EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC
DOI: 10.1016/j.amjsurg.2012.07.004
Keywords
Intestinal development; Schlafen; Short-gut syndrome; Adaptation
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Funding
- NIDDK NIH HHS [R01 DK096137, R56 DK096137] Funding Source: Medline
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BACKGROUND: Understanding gut development may illuminate the adaptive response to massive small-bowel resection and facilitate enteral nutrition. We reported that Schlafen-3 (Slfn3) mediates differentiation in vitro in rat intestinal epithelial. We hypothesized that Slfn3 is involved in intestinal development in vivo. METHODS: We removed fetal intestines, liver, and lungs on day 20 of gestation, at birth, and on postnatal days 1 and 5. Expression of Slfn3, markers of intestinal differentiation, and Slfn5, to address specificity, were determined by quantitative reverse-transcription polymerase chain reaction. RESULTS: Villin expression increased on days 1 and 5 (8.7 +/- .6 and 5.4 +/- .4, respectively; P < .01). Intestinal Slfn3 expression was increased substantially after birth (2.1- +/- .5-fold) and on days 1 and 5 (P < .02). Slfn3 was higher after birth in liver and lung but decreased sharply thereafter. Slfn5 expression was mostly unchanged. CONCLUSIONS: The data suggest that the developmental/maturation effects we observed correlate with Slfn3 but not Slfn5 and are more relevant to the intestines. A better understanding of how Slfn3 promotes intestinal differentiation could help promote intestinal maturation, improving outcomes in children or adults with short-gut syndrome. Published by Elsevier Inc.
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