Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 73, Issue 5, Pages 390-401Publisher
WILEY
DOI: 10.1111/aji.12341
Keywords
Antiphospholipid; obstetric; TLR4; trophoblast
Categories
Funding
- Nuffield Foundation Oliver Bird Rheumatism Programme
- Arthritis Research UK [19423]
- Arthritis Research UK Centre [20164]
- National Institute for Health Research University College London Hospitals Biomedical Research Centre
- Versus Arthritis [19423] Funding Source: researchfish
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ProblemSome patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells. Method of studyHTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFB and ERK; and expression of interleukin (IL)-8 and IL-6. ResultsVT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression. ConclusionsVT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.
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