Article
Medicine, Research & Experimental
Jin Zou, Can Xu, Zhen-Wang Zhao, Shan-Hui Yin, Gang Wang
Summary: This study found that Asprosin regulates the expression of ABCA1 and ABCG1 by activating the p38/Elk-1 signaling pathway, thereby reducing lipid accumulation in macrophages and decreasing atherosclerotic burden in apoE(-/-) mice.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Yu -Wei Lai, Shih-Wei Wang, Chia-Liang Lin, Shiou-Sheng Chen, Kuan-Hung Lin, Yen-Tung Lee, Wei-Cheng Chen, Yi-Hsien Hsieh
Summary: This study investigates the effect of Asiatic Acid (AA) on prostate cancer metastasis and its molecular mechanisms. The results show that AA inhibits the migratory and invasive capabilities of prostate cancer cells by affecting Snail. Additionally, AA inhibits the phosphorylation of MEK3/6 and p38MAPK, and p38MAPK influences prostate cancer cell metastasis.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shirui Huang, Jizhen Li, Shuang Wu, Zhijian Zheng, Cong Wang, Hongyan Li, Lingling Zhao, Xiaodong Zhang, Haishan Huang, Chuanshu Huang, Qipeng Xie
Summary: Colorectal cancer (CRC) is a common malignant tumor in the gastrointestinal tract and has attracted extensive research attention. The C4orf19 gene is downregulated in CRC tissues, and its expression levels are positively correlated with patient prognosis. Ectopic expression of C4orf19 inhibits CRC cell growth by targeting the Keap1/USP17/Elk-1/CDK6 axis.
Review
Biochemistry & Molecular Biology
Gerald Thiel, Oliver G. Roessler
Summary: Pancreatic beta-cells are critical for insulin production and their loss is a key feature of diabetes mellitus. Identifying proteins that regulate the number of beta-cells is crucial for diabetes research. This review article summarizes the results of three transgenic mouse models, showing that the transcription factors Elk-1 and Egr-1, as well as the protein phosphatase calcineurin, play a role in controlling beta-cell numbers and glucose homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Wu Chen, Sheng Zhao, Ji Xing, Xiangjun Zhou, Siqi Li, Yuqi Xia, Tianbao Song, Wei Li, Fan Zou, Fan Cheng
Summary: This study found that BMAL1 is involved in the regulation of renal fibrosis and renal interstitial inflammation in obstructed kidneys. Downregulation of BMAL1 promoted the production of extracellular matrix proteins and proinflammatory factors, while upregulation inhibited this process. The ERK1/2/ELK-1/Egr-1 axis was identified as an important pathway for BMAL1 to play a regulatory role.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Health Care Sciences & Services
Melis Savasan Sogut, Chitra Venugopal, Basak Kandemir, Ugur Dag, Sujeivan Mahendram, Sheila Singh, Gizem Gulfidan, Kazim Yalcin Arga, Bayram Yilmaz, Isil Aksan Kurnaz
Summary: Elk-1, a member of the ETS domain superfamily, is a transcription factor involved in neuroprotection, neurodegeneration, and brain tumor proliferation. It regulates known targets like c-fos and egr-1, as well as SMN, SOD1, PSEN1, and CD133. Microarray analysis revealed Elk-1's significant regulation of genes related to nervous system development, pluripotency, and apoptosis. Elk-1 also plays a role in the self-renewal of CD133+ BTICs.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Immunology
Eric D. Cassmann, S. Jo Moore, Justin J. Greenlee
Summary: The study investigated the host range of Chronic Wasting Disease (CWD) by inoculating Suffolk sheep with brain homogenate from CWD-positive white-tailed deer, leading to immunoreactivity against the misfolded prion protein in lymphoid tissue in one out of seven sheep after 60 months, as confirmed by mouse bioassay.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Forestry
Elisa Fuentes-Montemayor, Kirsty J. Park, Kypfer Cordts, Kevin Watts
Summary: The study revealed that woodland creation sites go through stages of "stand initiation", "stem exclusion", and "understorey reinitiation", gradually becoming more similar to mature ancient woodlands over time. Structural heterogeneity, average tree size, and tree density were found to vary significantly among different woodland developmental stages. Implementing woodland management practices such as creating canopy gaps and reducing grazing pressure can accelerate this transition, increase biodiversity value, and enhance adaptation and resilience to climate change.
Article
Ecology
Luke E. Painter, Robert L. Beschta, William J. Ripple
Summary: The recruitment of aspen saplings increased as elk browsing decreased after the reintroduction of wolves in Yellowstone National Park in 1995-96. The results support the idea of a trophic cascade benefiting aspen, countering claims that recent aspen recovery has been exaggerated.
Article
Biochemistry & Molecular Biology
Sung-Ho Go, Seung Bae Rho, Dong-Wha Yang, Boh-Ram Kim, Chang Hoon Lee, Seung-Hoon Lee
Summary: This study identified the interaction between the Elk-1 factor and the human papillomavirus (HPV)-18 E7 oncoprotein using the yeast two-hybrid method. The interaction enhanced the transcriptional activity of Elk-1 and increased the expression of target genes, leading to cell proliferation.
BIOMOLECULES & THERAPEUTICS
(2022)
Article
Biodiversity Conservation
Benjamin N. Sacks, Gunmeet K. Bali, Zachary T. Lounsberry, Stevi L. Vanderzwan
Summary: A suite of 96 SNP markers was developed through high-throughput sequencing for differentiation of individual tule elk and other subspecies in California, aiding genetic monitoring efforts.
CONSERVATION GENETICS RESOURCES
(2021)
Article
Ecology
Jon S. Horne, Sarah J. Thompson
Summary: Studying wildlife shortly after parturition is challenging, but a ratio-based model has been developed to estimate the overall survival rate and timing of mortality for neonates without relying on invasive or expensive techniques.
Article
Veterinary Sciences
Yu-Ran Lee, Yong-Chan Kim, Sae-Young Won, Min-Ju Jeong, Kyung-Je Park, Hoo-Chang Park, In-Soon Roh, Hae-Eun Kang, Hyun-Joo Sohn, Byung-Hoon Jeong
Summary: This study identified a novel risk factor, the S100G SNP, for chronic wasting disease (CWD) in elk, through the analysis of the PRNP gene.
VETERINARY RESEARCH
(2023)
Article
Health Care Sciences & Services
Yigit Koray Babal, Basak Kandemir, Isil Aksan Kurnaz
Summary: The ETS domain family of transcription factors is vital in various biological processes and commonly dysregulated in cancers. Analysis of microarray data from glioma patients led to identification of novel biomarkers ETV1, ELK3, ETV4, ELF4, and ETV6 for different glioma grades. Comparative analysis of gene regulatory networks of ETS transcription factors revealed unique and common networks with previous microarray studies involving Elk-1-VP16 or PEA3-VP16 overexpression in neuroblastoma cell lines.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemical Research Methods
Charles Ducker, Manohar Ratnam, Peter E. Shaw, Robert Layfield
Summary: Post-translational modifications (PTMs) play a crucial role in protein folding and activity. Recreating physiologically relevant PTM profiles on recombinantly-expressed proteins is essential for functional analysis. This study compared PTMs on human ELK-1 expressed in different systems and found that phosphorylation and acetylation significantly impact its DNA binding and interaction with androgen receptor. The choice of expression system can be tailored for specific applications and PTMs of ELK-1 show potential crosstalk and antagonism.
PROTEIN EXPRESSION AND PURIFICATION
(2023)