Article
Pharmacology & Pharmacy
J. Y. Fu, C. X. Jiang, M. Y. Wu, R. Y. Mei, A. F. Yang, H. P. Tao, X. J. Chen, J. Zhang, L. Huang, X. F. Zhao
Summary: Theabrownin (TB) has shown potential as an antitumor agent in glioma cells by significantly reducing cell viability and inducing cell cycle arrest through modulation of various proteins. Different mechanisms related to c-myc and P53 were proposed for cell cycle arrest in different glioma cell lines. TB could be a novel candidate drug for the treatment of gliomas based on these findings.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Meng Qu, Guoxin Zhang, Han Qu, Alexander Vu, Raymond Wu, Hidekazu Tsukamoto, Zhenyu Jia, Wendong Huang, Heinz-Josef Lenz, Jeremy N. Rich, Steve A. Kay
Summary: Hepatocellular carcinoma (HCC) is a global health challenge with increasing incidence worldwide. The circadian clock has been shown to play a key role in hepatocarcinogenesis, but the cellular and molecular mechanisms underlying the HCC-clock crosstalk are unknown. In this study, we found that HCC cells rely on the circadian clock transcription factors BMAL1 and CLOCK for cell growth, and down-regulation of these factors induces apoptosis and cell cycle arrest. Mechanistically, inhibiting BMAL1/CLOCK dysregulates cell cycle regulators Wee1 and p21, leading to tumor cell death. Our findings provide insights into the cellular impact of clock proteins in HCC oncogenesis and suggest a potential therapeutic approach based on modulation of the circadian clock.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Multidisciplinary
Jin-yi Liu, Wei-qi Fu, Xiang-jin Zheng, Wan Li, Li-wen Ren, Jin-hua Wang, Cui Yang, Guan-hua Du
Summary: Avasimibe demonstrated potent anticancer effects on human glioblastoma cells by inhibiting proliferation, inducing apoptosis, and regulating multiple signaling pathways. The drug showed promising potential as a chemotherapy treatment for glioblastoma.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Cell Biology
Qiong Liu, Lingying Chen, Wenjun Yin, Yuehua Nie, Penghui Zeng, Xin Yang
Summary: The study demonstrated that ginkgetin has an anti-tumor effect on human hepatocellular carcinoma cell lines by inducing apoptosis through affecting the balance between cell proliferation and apoptosis.
Article
Chemistry, Multidisciplinary
Edgar Uhl, Friederike Wolff, Sriyash Mangal, Henry Dube, Esther Zanin
Summary: The research reveals that the versatile proteasome inhibitor MG132, inactivated with a photolabile protection group, can be restored with blue light irradiation, causing cancer cells to arrest during metaphase of the cell cycle or undergo apoptosis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biotechnology & Applied Microbiology
Qiang Wang, Liuming Zhang, Dejun Ji, Jingwen Qu, Jian Wang, Hao Zhang, Yongjun Li
Summary: This study investigated the regulation and function of CMTM3 in hair follicle stem cells. It was found that CMTM3 re-expression suppressed HFSC proliferation by inducing cell cycle arrest and apoptosis, while downregulation of CMTM3 promoted proliferation. Transcriptome analysis identified multiple genes and pathways involved in this process, suggesting a complex crosstalk between pathways and transcription factors mediated by CMTM3.
Article
Oncology
Si-Liang Wei, Jing-Jing Ye, Li Sun, Lei Hu, Yuan-Yuan Wei, Da-Wei Zhang, Meng-Meng Xu, Guang-He Fei
Summary: This study identified a novel mechanism involving circKIF20B/miR-615-3p/MEF2A signaling axis in the progression of gefitinib resistance in NSCLC. Downregulation of circKIF20B promotes resistance to gefitinib, while upregulation restores sensitivity. Exosomal circKIF20B may serve as a potential therapeutic target and liquid biopsy candidate in gefitinib-resistant NSCLC.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Jeyce Kelle Ferreira de Andrade, Alexandre Jose da Silva Goes, Vanessa Xavier Barbosa, Mariza Severina de Lima Silva, Mariana Aragao Matos Donato, Christina Alves Peixoto, Gardenia Carmen Gadelha Militao, Teresinha Goncalves da Silva
Summary: Thiosemicarbazones derived from beta-lapachone showed potent cytotoxic effects on leukemia cells, with BV3 being the most selective compound, exhibiting high toxicity to tumor cells and low toxicity to normal cells. These compounds have the potential to be promising candidates for anticancer drugs.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Tai-Hsin Tsai, Kuan-Ting Lee, Yi-Chiang Hsu
Summary: Cucurbitacin I (JSI-124) derived from Cucurbitaceae has the potential to induce apoptosis and cell cycle arrest in glioblastoma multiforme (GBM) cells. JSI-124 effectively reduced cell viability in GBM cells, leading to apoptosis and cell cycle arrest.
Article
Multidisciplinary Sciences
Francisco J. Sanchez-Rivera, Jeremy Ryan, Yadira M. Soto-Feliciano, Mary Clare Beytagh, Lucius Xuan, David M. Feldser, Michael T. Hemann, Jesse Zamudio, Nadya Dimitrova, Anthony Letai, Tyler Jacks
Summary: The level of mitochondrial apoptotic priming is a critical determinant of cell fate upon p53 reactivation, with cells having high initial priming tending to undergo apoptosis and cells with low priming tending to survive and arrest in the cell cycle. Manipulating the priming levels through BCL-2 or BCL-XL expression or inhibition can affect the outcome of p53 restoration, highlighting mitochondrial apoptotic priming as a key factor in determining cell fate. Moreover, less primed cells can be forced into apoptotic cell fate following p53 activation using p53-independent drugs that increase apoptotic priming.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Eva Kiss, Gertrud Forika, Magdolna Dank, Tibor Krenacs, Zsuzsanna Nemeth
Summary: The study found that dietary methyl donors may improve the physiology of pancreatic cancer patients and could be used as an intervention therapy. Methyl donors reduced the aggressiveness and proliferation of cancer cells while decreasing inflammation and metastatic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Haini Wang, Junli Zuo
Summary: Overexpressed survivin is associated with worse survival of several types of human tumors. In this study, the antitumor activity of shikonin in non-small-cell lung cancer (NSCLC) by regulating survivin pathway was investigated. Result showed that shikonin inhibited the NSCLC H1299 cell proliferation in a dose-dependent manner. Moreover, shikonin fits well with survivin by molecular docking. Shikonin also inhibited the mRNA expression and protein level of survivin in H1299 cells. Shikonin arrested H1299 cell cycle at the G0/G1 phase by regulating CDK/cyclin family members. In addition, shikonin regulated the expression of X-linked inhibitor of apoptosis- (XIAP-) mediated caspases 3 and 9, thus leading to the damage of mitochondrial membrane potential and induction of H1299 cell apoptosis. Overall, shikonin inhibited H1299 cell growth by inducing apoptosis and blocking the cell cycle. The underlying mechanism involves targeting survivin, which subsequently regulates the protein expression of XIAP/caspase 3/9, CDK2/4, and cyclin E/D1. Thus, shikonin, a survivin inhibitor, is a promising therapeutic strategy in NSCLC treatment.
ANALYTICAL CELLULAR PATHOLOGY
(2021)
Article
Plant Sciences
Hyesu Jeon, Diem Thi Ngoc Huynh, Naehwan Baek, Thuy Le Lam Nguyen, Kyung-Sun Heo
Summary: The study showed that G-Rg2 has significant anticancer effects on MCF-7 breast cancer cells by increasing ROS-AMPK signaling pathway and inhibiting ERK1/2 and Akt activation-mediated cell proliferation and cell cycle progression.
Article
Multidisciplinary Sciences
Vartika Srivastava, Mohmmad Younus Wani, Abdullah Saad Al-Bogami, Aijaz Ahmad
Summary: The study synthesized six novel piperidine derivatives and tested their antifungal activity and mechanism of action against clinical isolates of C. auris. Among them, pta1, pta2, and pta3 demonstrated the highest activity, lower toxicity, and the ability to induce apoptotic cell death and cell cycle arrest in C. auris.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Hai-bin Nong, Ya-nan Zhang, Yi-guang Bai, Qiong Zhang, Ming-fu Liu, Quan Zhou, Zhuo-hua Shi, Gao-feng Zeng, Shao-Hui Zong
Summary: The study demonstrated that ADA significantly inhibited cell proliferation, migration, invasiveness, induced S-phase arrest and apoptosis in prostate cancer cells. ADA also regulated the expression of S-phase associated proteins and DNA damage markers, suggesting its potential anti-tumor effect through the DNA damage/p53 pathway. Additionally, ADA effectively inhibited tumor growth and bone destruction in mice models.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Surgery
Isaac N. Naazie, Isibor Arhuidese, Mohammad S. Abdelgawwad, Jonathan Unkart, Michael S. Conte, Mahmoud B. Malas
Summary: Different patterns of RAASI usage have different associations with outcomes in patients undergoing lower extremity bypass for peripheral arterial disease. Isolated preoperative RAASI use is associated with worse mortality and amputation-free survival, while continuous RAASI use is associated with improved outcomes.
ANNALS OF VASCULAR SURGERY
(2023)
Article
Surgery
Sanuja Bose, Chen Dun, Rebecca Sorber, David P. Stonko, Alex J. Solomon, James H. Black, Ying-Wei Lum, Michael S. Conte, Martin A. Makary, Caitlin W. Hicks
Summary: This study examined the practice patterns of tibial peripheral vascular interventions (PVIs) for the treatment of claudication in the United States. The results showed that tibial PVI was mainly performed by nonvascular surgeons in high-volume practices and high-reimbursement settings. There is a need to reevaluate the indications, education, and reimbursement policies surrounding these procedures.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Surgery
Lewis Meecham, Mathew Popplewell, Gareth Bate, Huw O. B. Davies, Akio Kodama, Michael S. Conte, Andrew W. Bradbury
Summary: The Global Limb Anatomic Staging System (GLASS) is a new method of quantifying the severity of lower limb disease in patients with chronic limb-threatening ischemia. This study found that worsening GLASS stage was associated with reduced immediate technical success and worse outcomes in terms of freedom from reintervention and major adverse limb events. However, further refinement of GLASS may be necessary to fully utilize it as a decision-making tool in clinical practice.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Surgery
Emanuel A. Jaramillo, Eric J. T. Smith, Zachary A. Matthay, Katherine M. Sanders, Jade S. Hiramoto, Warren J. Gasper, Michael S. Conte, James C. Iannuzzi
Summary: Despite controlling for disease severity, racial and ethnic disparities persist in major adverse limb events (MALE) after peripheral vascular intervention (PVI) for chronic limb threatening ischemia (CLTI). This suggests that disease severity does not account for the disparities in outcomes.
JOURNAL OF VASCULAR SURGERY
(2023)
Article
Surgery
Eric J. T. Smith, Warren J. Gasper, Peter A. Schneider, Emily Finlayson, Louise C. Walter, Ken E. Covinsky, Michael S. Conte, James C. Iannuzzi
Summary: This study examines cognitive impairment in patients with peripheral arterial disease (PAD) and finds that it is common but often unrecognized. Risk factors for cognitive impairment include age, race, hypertension, prior stroke/TIA, diabetes treated with insulin, and post-traumatic stress disorder (PTSD). The study suggests that cognitive impairment is an underdiagnosed issue in PAD patients and calls for further research on its impact and management.
ANNALS OF VASCULAR SURGERY
(2023)
Review
Engineering, Biomedical
Patrick M. Wieruszewski, Shea A. Macielak, Scott D. Nei, Rajat N. Moman, Troy G. Seelhammer, Christoph G. S. Nabzdyk, Danielle J. Gerberi, Kristin C. Mara, W. Michael Hooten, Erica D. Wittwer
Summary: In clinical studies comparing heparin and bivalirudin in adult patients receiving ECMO, it was found that those who received heparin were more likely to experience circuit-related thrombosis and death, but there were no differences in major bleeding events between the two groups.
Letter
Engineering, Biomedical
Patrick M. Wieruszewski, James R. Neal, Christoph G. S. Nabzdyk, Troy G. Seelhammer
Editorial Material
Surgery
Michael S. Conte
SEMINARS IN VASCULAR SURGERY
(2023)
Editorial Material
Anesthesiology
Misty Radosevich, Etienne J. Couture, Christoph Nabzdyk
JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA
(2023)
Article
Multidisciplinary Sciences
Jose L. Lopez, Joel L. Ramirez, Tuan Anh Phu, Phat Duong, Laura Bouchareychas, Christina R. Kuhrau, Pei-Yu Lin, Walter L. Eckalbar, Andrea J. Barczak, Joshua D. Rudolph, Lenka Maliskova, Michael S. Conte, Shant M. Vartanian, Robert L. Raffai, Adam Z. Oskowitz
Summary: This study aimed to identify novel biomarkers for abdominal aortic aneurysms (AAAs) by characterizing the expression profile of microRNAs (miRNA) in circulating exosomes. The study found that miR-122-5p levels were significantly reduced in the exosomes of AAA patients. This suggests that miR-122-5p could potentially be used as a diagnostic biomarker and have implications in AAA pathogenesis.
Article
Peripheral Vascular Disease
Justin Chin-Bong Choi, Jorge Miranda, Erin Greenleaf, Michael S. Conte, Marie D. Gerhard-Herman, Joseph L. Mills, Neal R. Barshes
Summary: This study used the WIfI system to predict limb loss and peripheral artery disease in patients with foot ulcers or gangrene. It found that a toe-brachial index <0.7 and minimum ankle-brachial index <0.9 were effective in identifying patients with severe disease, with high sensitivity rates. The diagnostic accuracy of noninvasive measures varied in identifying different degrees of disease. It also found that the presence of medial artery calcification significantly reduced the sensitivity of other noninvasive parameters.
Article
Surgery
Clara M. Gomez-Sanchez, Michael S. Conte
Summary: Chronic limb-threatening ischemia is difficult to treat due to the complexity of patients, diversity of limb presentations, and complicated arterial pathology. Vascular surgeons require a wide range of skills to tailor interventions to the specific needs of each patient. Inframalleolar bypass is a tool for patients with extensive arterial occlusive disease below the knee, but it requires systematic surgical planning and a high level of technical competence. We describe our approach to inframalleolar bypass for limb preservation in suitable patients with advanced tibial artery disease.
JOURNAL OF VASCULAR SURGERY CASES INNOVATIONS AND TECHNIQUES
(2023)
Review
Endocrinology & Metabolism
Vivienne Chuter, Nicolaas Schaper, Joseph Mills, Robert Hinchliffe, David Russell, Nobuyoshi Azuma, Christian-Alexander Behrendt, Edward J. Boyko, Michael S. Conte, Misty Humphries, Lee Kirksey, Katharine C. McGinigle, Sigrid Nikol, Joakim Nordanstig, Vincent Rowe, Jos C. van den Berg, Maarit Venermo, Robert Fitridge
Summary: Early diagnosis and ongoing monitoring and treatment of peripheral artery disease (PAD) is crucial for reducing the risk of foot ulcer development, non-healing of wounds, infection, amputation, and cardiovascular complications in patients with diabetes. Various non-invasive bedside tests are available for diagnosing PAD, but the most accurate test and its reliability for ongoing monitoring are still uncertain. This systematic review aimed to determine the diagnostic accuracy of non-invasive bedside tests for identifying PAD and their reliability in adults with diabetes. The review included 40 studies on diagnostic accuracy and 7 studies on reliability. Ankle-brachial index (ABI) was the most investigated test. The review found that an ABI <0.9 increases the likelihood of disease, while an ABI within the normal range (≥0.90 and <1.3) does not exclude PAD. Toe-brachial index (TBI) and Doppler waveforms in the pedal arteries were also associated with PAD presence. Several bedside tests showed acceptable reliability, but the margin of error was wide. No single or combination of bedside tests demonstrated superior diagnostic accuracy for PAD in patients with diabetes. However, an ABI of 1.3, TBI <0.70, and absent or monophasic pedal Doppler waveforms can help identify the presence of disease.
DIABETES-METABOLISM RESEARCH AND REVIEWS
(2023)
Article
Surgery
Cindy Huynh, Iris Liu, Rym El Khoury, Bo Zhou, Hillary Braun, Michael S. Conte, Jade Hiramoto
Summary: By incorporating a common iliac artery calcification score into the VQI prediction model, this study improved the accuracy of the 2-year risk calculator. Preoperative assessment of iliac artery calcification may assist with risk stratification and clinical decision-making in patients with chronic limb-threatening ischemia (CLTI) undergoing infrainguinal revascularization.
JOURNAL OF VASCULAR SURGERY
(2023)
Editorial Material
Surgery
Michael S. Conte, Leigh Ann O'Banion
JOURNAL OF VASCULAR SURGERY
(2023)