Article
Agriculture, Multidisciplinary
Yike Jiang, Ting Zheng, Weiping Jin, Yuxin Shi, Qingrong Huang
Summary: This study utilized chitosan and caseinophosphopeptides to improve the intestinal permeability of theaflavin-3,3'-digallate. The complexation between chitosan and caseinophosphopeptides was mainly driven by electrostatic interaction. The optimized nanocomplex successfully encapsulated theaflavin-3,3'-digallate and enhanced its intestinal permeability.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Ayuni Yussof, Brian Cammalleri, Oluwanifemi Fayemiwo, Sabrina Lopez, Tinchun Chu
Summary: In this study, it was found that Theaflavin-3,3'-digallate (TFDG) has antibacterial and anti-spore activities, and can bind to bacterial proteins to reduce the expression of related genes. This suggests the potential application of TFDG as a broad-spectrum antibacterial and anti-spore agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Jinghui Zhou, Changwei Liu, Shimin Zhao, Ya Liu, Sheng Zhang, Qiang Zhao, Fen Wang, Gang Xu, Jianan Huang, Zhonghua Liu
Summary: Theaflavin-3,3'-digallate (TFDG) from black tea has various health benefits, but its application is limited due to low yields. In this study, tyrosinases from Bacillus megaterium were used to synthesize TFDG through directed evolution and high-throughput screening. Mutant Bmtyrc-3 showed significantly improved catalytic efficiency and higher production of TFDG. The broader substrate binding area of Bmtyrc-3 resulted in increased activity of substrates EGCG and ECG.
Article
Biochemistry & Molecular Biology
Ying Huang, Changzheng Gao, Wei Song, Wanqing Wei, Xiulai Chen, Cong Gao, Jia Liu, Jing Wu, Liming Liu
Summary: In this study, a microbial polyphenol oxidase from Bacillus megaterium was used to synthesize theaflavin-3,3 '-digallate (TFDG). By optimizing the conformation of the O-O bond dissociation, the catalytic efficiency of the enzyme was improved. The optimized conditions resulted in a significantly higher production of TFDG, indicating the great potential of this method in the synthesis of TFDG and other TFs.
Article
Immunology
Lei Zhang, Wenming Li, Zhenyang Hou, Zhidong Wang, Wei Zhang, Xiaolong Liang, Zerui Wu, Tianhao Wang, Xin Liu, Xiaole Peng, Xing Yang, Huilin Yang, Dechun Geng
Summary: By modulating autophagy, Theaflavin-3,3'-digallate (TFDG) can alleviate inflammation and promote anti-inflammatory M2 macrophage polarization, thus protecting joints against collagen-induced arthritis.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Chemistry, Applied
Xia Yu, Xinghong Cai, Shuang Li, Liyong Luo, Jie Wang, Min Wang, Liang Zeng
Summary: By utilizing various experimental techniques such as fluorescence spectroscopy and UV-vis absorption spectroscopy, the interaction mechanism between theaflavin-3,3'-digallate (TFDG) and bovine serum albumin (BSA) was studied, demonstrating that hydrophobic interactions and hydrogen bonds play a dominant role in their interaction. This research provides the possibility for further development of nanoparticles with superior biochemical properties and potential exploration of BSA as a transport carrier for TFDG.
Article
Nutrition & Dietetics
Hui Zhou, Yuanyuan Wu, Eunhye Kim, Haibo Pan, Puming He, Bo Li, Yi Charlie Chen, Youying Tu
Summary: TF3, the most crucial theaflavin monomer in black tea, was found to effectively combat diabetes by regulating phosphoenolpyruvate carboxykinase and glucokinase, while also promoting beta cell regeneration in diabetic zebrafish.
Article
Nutrition & Dietetics
Hui Zhou, Chen Xia, Yaqing Yang, Hasitha Kalhari Warusawitharana, Xiaohui Liu, Youying Tu
Summary: Theaflavin-3,3'-digallate (TF3), a compound found in black tea, has been found to have anti-coronary heart disease effect. In this study, TF3 was shown to reduce cell size and fetal gene mRNA level in a heart failure cell model, potentially preventing pathological cardiac hypertrophy. TF3 may be a promising natural compound for the treatment of heart failure.
Article
Biotechnology & Applied Microbiology
Changwei Liu, Jinghui Zhou, Jianan Huang, Wei Xu, Zhonghua Liu
Summary: Polyphenol oxidase and its isoenzymes in the tea tree were studied for their expression and activity towards tea polyphenol substrates. Different methods, including protein sequence treatment and immobilization, were used to enhance the activity of the recombinant enzyme. Results showed that CsPPO1, CsPPO2, CsPPO3, and CsPPO4 were expressed as inclusion bodies and exhibited improved activity. Immobilization treatment significantly increased the efficiency of the CsPPO2 enzyme.
FERMENTATION-BASEL
(2023)
Article
Psychology, Biological
Yichou Cao, Yunyi Zhang, Zehan Jia, Huining Jia, Yuanchen Sun, Hongxia Yuan, Yongle Bian, BingJie Xu, Jing Fu, Fenju Qin
Summary: Age-related neurodegenerative diseases with learning and memory deficits are increasing due to population aging. Cellular oxidative stress is a common mechanism in these diseases, and plant extracts with antioxidant activity have shown therapeutic potential. Theaflavin-3,3'-digallate (TFDG) has been identified as a potent antioxidant with the ability to improve learning and memory impairments in the D-galactose model of age-related neurodegeneration.
PHYSIOLOGY & BEHAVIOR
(2023)
Article
Cell Biology
Giulia Pozzi, Valentina Presta, Elena Masselli, Giancarlo Condello, Samuele Cortellazzi, Maria Luisa Arcari, Cristina Micheloni, Marco Vitale, Giuliana Gobbi, Prisco Mirandola, Cecilia Carubbi
Summary: Reactive oxygen species (ROS) are key players in physiological processes and can affect myogenic differentiation. Protein kinase C epsilon (PKCe) is involved in muscle stem cell differentiation and skeletal muscle regeneration. PKCs have a role in redox biology and may regulate ROS production. Our study suggests that PKCe is a molecular link between redox homeostasis and myogenic differentiation. We found that PKCe interacts with Nrf2 to activate antioxidant signaling, preventing ROS accumulation and promoting myogenic differentiation.
Article
Biotechnology & Applied Microbiology
Chaoping Yue, Zhiwei Su, Baoyan Tai, Hui Tang, Wangbao Da, Hongli Xu, Huawei Zeng, Bingyue Xin, Xin Zeng
Summary: This study found that a proper dose of intracellular ROS (about 3.3 μmol H2O2/g DCW) could induce physiological modifications to promote the production of epsilon-PL. The proper dose of ROS activated the metabolism of the pentose phosphate pathway and promoted the biosynthesis of epsilon-PL and lysine. Additionally, energy provision was ensured by the activated metabolism of the TCA cycle. These findings have theoretical and practical significance for industrial production of epsilon-PL.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Loka Reddy Velatooru, Rei J. Abe, Masaki Imanishi, Young Jin Gi, Kyung Ae Ko, Kyung-Sun Heo, Keigi Fujiwara, Nhat-Tu Le, Sivareddy Kotla
Summary: Activation of focal adhesion kinase (FAK) in endothelial cells plays a crucial role in vascular diseases such as atherosclerosis. The study shows that disturbed flow (D-flow) induces endothelial activation and senescence through the upregulation of FAK SUMOylation, and mutating this site significantly inhibits these processes.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Environmental Sciences
Yajie Niu, Junru Li, Ye Zhao, Di Xin, Xue Gao, Shuyong Zhang, Jing Guo
Summary: This study found that the PeMPK17 gene is activated under pHBA stress and protects poplar cell membranes by regulating ROS levels. Overexpression of PeMPK17 also promotes the accumulation of osmoprotectants, aiding in the regulation of ROS balance under pHBA treatment.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Agronomy
Rupal Singh Tomar, Sunita Kataria, Anjana Jajoo
Summary: This article explores the recent research on signaling and mechanisms for salt tolerance in plants, focusing on the role of the salt overly sensitive system (SOS) and related antiporters proteins and mitogen-activated protein kinase cascades under salt stress. It also highlights the differential expression of various antioxidative enzymes in C3, C4 and CAM plants.
JOURNAL OF AGRONOMY AND CROP SCIENCE
(2021)
Article
Food Science & Technology
Min Li, Daxiang Li, Yuling Tai, Chenchen Gu, Yasai Song, Weiting Jiao, Jingming Ning, Chaoling Wei, Xungang Gu, Chi-Tang Ho, Jamal U. Ddin Hajano, Xiaochun Wan
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2018)
Article
Agriculture, Multidisciplinary
Yijun Wang, Zhipeng Kan, Henry J. Thompson, Tiejun Ling, Chi-Tang Ho, Daxiang Li, Xiaochun Wan
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Editorial Material
Agriculture, Multidisciplinary
Daxiang Li, Yu Wang, Zhengzhu Zhang, Xiaochun Wan, Chi-Tang Ho
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Agriculture, Multidisciplinary
Ruru Wang, Menchao Xiao, Yujing Zhang, Chi-Tang Ho, Xiaochun Wan, Daxiang Li, Zhongwen Xie
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Agriculture, Multidisciplinary
Yafang Wang, Yanqiu Zuo, Siyu Deng, Feng Zhu, Qin Liu, Ruru Wang, Tao Li, Huimei Cai, Xiaochun Wan, Zijian Xie, Zhongwen Xie, Daxiang Li
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Review
Chemistry, Applied
Hao Jiang, Feng Yu, Li Qin, Na Zhang, Qiong Cao, Wilfried Schwab, Daxiang Li, Chuankui Song
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2019)
Review
Nutrition & Dietetics
Daxiang Li, Ruru Wang, Jinbao Huang, Qingshuang Cai, Chung S. Yang, Xiaochun Wan, Zhongwen Xie
Article
Food Science & Technology
Qianying Dai, Yurong Jiang, Sitong Liu, Jing Gao, Huozhu Jin, Huiqiang Wang, Mingji Xiao, Zhengzhu Zhang, Daxiang Li
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2019)
Article
Food Science & Technology
Junjun Xi, Liang Zhang, Chuanyi Peng, Jie Zhou, Yun Peng, Lingyun Xu, Bo Chen, Qilu Meng, Ruyan Hou, Daxiang Li, Yanling Zhang, Xiaochun Wan, Huimei Cai
LWT-FOOD SCIENCE AND TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Enhua Xia, Fangdong Li, Wei Tong, Hua Yang, Songbo Wang, Jian Zhao, Chun Liu, Liping Gao, Yuling Tai, Guangbiao She, Jun Sun, Haisheng Cao, Qiang Gao, Yeyun Li, Weiwei Deng, Xiaolan Jiang, Wenzhao Wang, Qi Chen, Shihua Zhang, Haijing Li, Junlan Wu, Ping Wang, Penghui Li, Chengying Shi, Fengya Zheng, Jianbo Jian, Bei Huang, Dai Shan, Mingming Shi, Congbing Fang, Yi Yue, Qiong Wu, Ruoheng Ge, Huijuan Zhao, Daxiang Li, Shu Wei, Bin Han, Changjun Jiang, Ye Yin, Tao Xia, Zhengzhu Zhang, Shancen Zhao, Jeffrey L. Bennetzen, Chaoling Wei, Xiaochun Wan
Article
Oncology
Erkang Jiang, Lianping Wei, Fang Tao, Mei Yu, Shu Wang, Xiuhong Zhou, Daxiang Li, Zhongwen Xie
CANCER CELL INTERNATIONAL
(2019)
Article
Pharmacology & Pharmacy
Yiyao Qi, Changjun Yang, Zhen Jiang, Yin Wang, Feng Zhu, Tao Li, Xiaochun Wan, Yunhui Xu, Zijian Xie, Daxiang Li, Sandrine Pierre
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2019)
Article
Food Science & Technology
Yun Peng, Junjun Xi, Yue Sun, Guijie Chen, Daxiang Li, Chuanyi Peng, Xiaochun Wan, Huimei Cai
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Nutrition & Dietetics
Yanzhong Zhang, Mingxing Gu, Ruru Wang, Menwan Li, Daxiang Li, Zhongwen Xie
NUTRITION & METABOLISM
(2020)