Article
Agriculture, Multidisciplinary
Tatsuya Suzuki, Seiji Yamato
Summary: Oxazosulfyl is a novel insecticide that acts by blocking voltage-gated sodium channels in insects, resulting in paralysis. This discovery sheds light on the mode of action of oxazosulfyl as a potential pest control agent in rice fields.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Milica Gusic, Klaus Benndorf, Christian Sattler
Summary: Our study reveals that each occupied binding site contributes to channel activation in P2X7 receptors. While the occupation of a single binding site can activate the channels, three bound ligands maximally stabilize the open state, suggesting a stepwise activation process for P2X7 receptors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Instruments & Instrumentation
Ian Delgadillo Bonequi, Abraham Stroschein, Lucas J. Koerner
Summary: This article describes a custom and open-source FPGA-based data acquisition system that is used for electrophysiology and closed-loop feedback experiments. The system combines FPGA acquisition and processing with high-speed analog and digital converters to enable real-time feedback. The digital approach simplifies experimental setup and repeatability by allowing for system identification and in situ tuning of filter bandwidths.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
Article
Biochemistry & Molecular Biology
Motoki Okui, Tatsuro Murakami, Hongxin Sun, Chiaki Ikeshita, Narisato Kanamura, Akiyuki Taruno
Summary: CALHM1 and 3 subunits are N-glycosylated at single Asn residues in their second extracellular loops, controlling the biosynthesis and gating kinetics of the CALHM1/3 channel. CALHM3 is reversibly palmitoylated at three intracellular Cys residues, with palmitoylation playing a critical role in CALHM1/3 activity.
Article
Neurosciences
Kevin Jehasse, Laurent Massotte, Sebastian Hartmann, Romain Vitello, Sofian Ringlet, Marie Vitello, Han Chow Chua, Stephan A. Pless, Dominique Engel, Jean-Francois Liegeois, Bernard Lakaye, Jochen Roeper, Vincent Seutin
Summary: The research showed that the compound XG selectively inhibits the pacemaking of dopamine neurons without affecting their bursting behavior. It demonstrated specificity in its effects on different types of neurons and seemed to target a small subthreshold XG-sensitive current carried by Na+ and Cl- ions. The molecular target of XG and its precise mechanism of action remain to be elucidated, but these findings represent a significant step towards understanding pacemaking in dopamine neurons.
Article
Biotechnology & Applied Microbiology
Sanung Eom, Shinhui Lee, Jiwon Lee, Minsu Pyeon, Hye Duck Yeom, Jung Hee Song, Eun Ji Choi, Moeun Lee, Junho H. Lee, Ji Yoon Chang
Summary: The study investigates the interaction between L-pyroglutamic acid and sour taste receptors, revealing the involvement of R299 of hPKD2L1. This finding provides a way to objectively measure the intensity of L-pyroglutamic acid and potentially create a database of taste.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Jiaru Fang, Xinwei Wei, Hongbo Li, Ning Hu, Xingxing Liu, Dongxin Xu, Tao Zhang, Hao Wan, Ping Wang, Xi Xie
Summary: Cardiovascular diseases pose a significant threat to human health, but drug development is time-consuming and costly. Researchers have developed a drug assessment platform based on an electrical-mechanical synchronized biosensing system, which allows for high-throughput and time-dependent assessment. The system can record and analyze dynamic responses of cardiomyocytes to drugs, enabling rapid and effective drug screening.
MICROSYSTEMS & NANOENGINEERING
(2021)
Review
Pharmacology & Pharmacy
Damian C. Bell, Luigi Leanza, Saverio Gentile, Daniel R. Sauter
Summary: Ion channels play key roles in various physiological processes, and their dysfunction can lead to channelopathies, including cancer. The field of ion channel and cancer research has rapidly grown in the past two decades, allowing us to gain deeper insights into the mechanisms of ion channels in cancer and develop therapeutic interventions.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Neurosciences
Z. Chen, D. H. Terman, S. P. Travers, J. B. Travers
Summary: Responses in the gustatory nucleus of the solitary tract (rNST) are modulated by synaptic interactions and membrane properties. The potassium current I-A is a potential modulator. This study explored the role of I-A in modulating the response to afferent stimulation and GABA-mediated inhibition in the rNST using transgenic mice expressing channelrhodopsin in GABAergic neurons.
Article
Biology
Peter R. Strege, Luke M. Cowan, Constanza Alcaino, Amelia Mazzone, Christopher A. Ahern, Lorin S. Milescu, Gianrico Farrugia, Arthur Beyder
Summary: Shear stress and patch suction can reversibly alter the kinetic properties and maximum current of NaChBac, suggesting it is mechanosensitive. Structural analysis reveals a large displacement of the intracellular gate, supporting the proposed mechanosensitive mechanism.
Article
Biology
Travis A. Hage, Alice Bosma-Moody, Christopher A. Baker, Megan B. Kratz, Luke Campagnola, Tim Jarsky, Hongkui Zeng, Gabe J. Murphy
Summary: Understanding cortical microcircuits requires thorough measurement of physiological properties of synaptic connections formed within and between diverse subclasses of neurons. In this study, the researchers combined optogenetic stimulation and multicellular recording to deeply characterize intralaminar and translaminar monosynaptic connections in the mouse visual cortex. They measured the reliability and specificity of the optogenetic stimulation and verified the connectivity measurements using paired recordings and targeted patching. The study revealed the abundance, spatial profiles, strength, and short-term dynamics of excitatory and inhibitory synaptic connections between genetically defined presynaptic populations. They found that layer 4 excitatory neurons and interneurons within L2/3 were the most common sources of input to L2/3 pyramidal cells, but also observed excitatory connections from layer 5 intratelencephalic neurons and translaminar inhibition from multiple interneuron subclasses.
Review
Biochemistry & Molecular Biology
Manan Bhatt, Angela Di Iacovo, Tiziana Romanazzi, Cristina Roseti, Raffaella Cinquetti, Elena Bossi
Summary: The heterologous expression of proteins in Xenopus laevis oocytes remains essential in many research fields. New approaches and revised protocols are applied to study membrane transporters, and both new and classic methods for investigating the activity and expression of Solute Carriers (SLC) are reviewed.
Article
Biology
Jan L. Muench, Fabian Paul, Ralf Schmauder, Klaus Benndorf
Summary: Inferring appropriate kinetic models for ion channel gating from ensemble currents is a challenging task due to limited data. We propose a Bayesian filter approach that specifies hidden Markov models for current and fluorescence data, which shows flexibility and superior performance compared to traditional methods. The approach can handle different noise distributions and incorporate orthogonal fluorescence data, providing more accurate parameter estimates.
Article
Biochemistry & Molecular Biology
Liying Dong, Qiqi Zhou, Qianqian Liang, Zhen Qiao, Yani Liu, Liming Shao, Kewei Wang
Summary: In this study, a novel TRPV1 agonist CPIPC was identified and shown to have analgesic activity in mice.
Article
Biochemistry & Molecular Biology
Daniel Barth, Andreas Lueckhoff, Frank J. P. Kuehn
Summary: Recent studies have found that PIP2 plays a crucial role in activating TRPM2 channels, with human TRPM2 being the least sensitive to PIP2 among different species variants. This suggests that changes in intramembranous PIP2 content can regulate the activity of TRPM2 channels in a species-specific manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Xu Cao, Bikash R. Pattnaik, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2018)
Article
Cell Biology
Bikash R. Pattnaik, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2009)
Article
Cell Biology
Xiaoming Zhang, Dongli Yang, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2011)
Article
Cell Biology
Bikash R. Pattnaik, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2012)
Article
Cell Biology
Wei Zhang, Xiaoming Zhang, Hui Wang, Anil K. Sharma, Albert O. Edwards, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2013)
Article
Ophthalmology
Dongli Yang, Xiaoming Zhang, Bret A. Hughes
EXPERIMENTAL EYE RESEARCH
(2008)
Article
Ophthalmology
Dongh Yang, Anuradha Swarninathan, Xiaoming Zhang, Bret A. Hughes
EXPERIMENTAL EYE RESEARCH
(2008)
Article
Ophthalmology
Xiaoming Zhang, Bret A. Hughes
EXPERIMENTAL EYE RESEARCH
(2013)
Article
Ophthalmology
Qitao Zhang, Feriel Presswalla, Kecia Feathers, Xu Cao, Bret A. Hughes, David N. Zacks, Debra A. Thompson, Jason M. L. Miller
EXPERIMENTAL EYE RESEARCH
(2019)
Article
Cell Biology
Xu Cao, Connor Baharozian, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2019)
Article
Cell Biology
Xu Cao, Manoocher Soleimani, Bret A. Hughes
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Ophthalmology
Dongli Yang, Susan G. Elner, Andrea J. Clark, Bret A. Hughes, Howard R. Petty, Victor M. Elner
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2011)
Article
Ophthalmology
Md Nawajes A. Mandal, Vidyullatha Vasireddy, G. Bhanuprakash Reddy, XiaoFei Wang, Sayoko E. Moroi, Bikash R. Pattnaik, Bret A. Hughes, John R. Heckenlively, Peter F. Hitchcock, Monica M. Jablonski, Radha Ayyagari
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2006)
Article
Ophthalmology
Md Nawajes A. Mandal, Vidyullatha Vasireddy, Monica M. Jablonski, XiaoFei Wang, John R. Heckenlively, Bret A. Hughes, G. Bhanuprakash Reddy, Radha Ayyagari
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2006)