Article
Pediatrics
Rajendra Prasad Anne, Venkateshwarlu Vardhelli, Srinivas Murki, Sai Kiran Deshabhotla, Tejo Pratap Oleti
Summary: Growth charts are useful in identifying at-risk neonates. The study found that INTERGROWTH-21(st) charts were more sensitive in identifying neonates developing complications, while population-based charts were more specific, accurate, and precise in differentiating neonates developing complications from those who did not.
INDIAN JOURNAL OF PEDIATRICS
(2022)
Article
Biochemistry & Molecular Biology
Christian Giommi, Marta Lombo, Nina Montik, Michela Paolucci, Valentina Notarstefano, Giovanni Delli Carpini, Andrea Ciavattini, Antonio Ragusa, Francesca Maradonna, Elisabetta Giorgini, Oliana Carnevali
Summary: Gestational diabetes mellitus (GDM) and small-for-gestational-age (SGA) are metabolic-related diseases that can occur during pregnancy. This study investigated the changes in the placental chorionic villi (CVs) of GDM and SGA patients. The results showed higher levels of SOD-1 and CAT in GDM placentae, which may be related to the hyperglycemic environment. Lipid composition and structure of the placenta were altered in both pathologies, with SGA inducing lipid peroxidation and reducing collagen deposition in the CVs. The cannabinoid system does not seem to be involved in these two pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xiaotong Yang, Paula A. Benny, Elorri Cervera-Marzal, Biyu Wu, Cameron B. Lassiter, Joshua Astern, Lana X. Garmire
Summary: Variations in telomere length have been linked to aging, stress, and diseases. This study investigated the association between placental telomere length and preeclampsia. The results suggest that placental telomere length is not significantly different between severe preeclampsia cases and healthy controls, but it is negatively correlated with gestational age and influenced by race.
Article
Medicine, General & Internal
Robert Eves, Dieter Wolke, Juliane Spiegler, Sakari Lemola
Summary: There is a lack of research on the nonlinear associations between birth weight percentiles and cognitive performance in infancy. This study aims to investigate the culturally invariant, nonlinear associations of birth weight percentiles and gestational age with later cognitive performance.
Article
Multidisciplinary Sciences
Meng Wang, Tingting Ren, Prince Marowa, Haina Du, Zongchang Xu
Summary: This study identified stable reference genes PP2A and TUA5 in S. glauca under different salt concentrations and tissues, providing a reliable method for normalizing gene expression. These reference genes were validated in germinating seeds of S. glauca, highlighting their utility in accurate gene expression profiling.
SCIENTIFIC REPORTS
(2021)
Article
Medical Laboratory Technology
Ankica Sekovanic, Adrijana Dorotic, Jasna Jurasovic, Daria Pasalic, Jelena Kovacic, Sandra Stasenko, Tatjana Mioc, Martina Piasek, Tatjana Orct
Summary: The study evaluated the efficacy of pre-amplification in increasing sensitivity of qPCR analysis for five candidate circulating miRNAs in mother-newborn plasma samples. Pre-amplification improved the detection of miRNAs, particularly miR-190b and miR-1537 showed varying results.
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medical Laboratory Technology
Luca Bernasconi, Peter Neyer, Michael Oberle, Bettina Schmid, Eileen Martin, Hans Fankhauser, Sebastian Haubitz, Angelika Hammerer-Lercher
Summary: This study assessed the diagnostic accuracy of a new fully automated SARS-CoV-2 antigen assay and explored the efficiency of a diagnostic algorithm combining antigen and molecular assays. The results showed that the assay had a sensitivity of 79.1% and specificity of 97.8%. By combining the antigen assay with RT-PCR, the number of tests could be reduced by 36.1% during the pandemic phase, leading to cost and time savings.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Veterinary Sciences
Siti Tasnim Makhtar, Sheau Wei Tan, Nur Amalina Nasruddin, Nor Azlina Abdul Aziz, Abdul Rahman Omar, Farina Mustaffa-Kamal
Summary: A specific TaqMan-based real-time RT-PCR assay targeting the N gene of FeMV was developed in this study, showing higher detection rate and sensitivity in early detection of FeMV infection.
BMC VETERINARY RESEARCH
(2021)
Article
Oncology
Aileen I. Fernandez, Niki Gavrielatou, Leena McCann, Saba Shafi, Myrto K. Moutafi, Sandra Martinez-Morilla, Ioannis A. Vathiotis, Thazin Nwe Aung, Vesal Yaghoobi, Yalai Bai, Yvonne G. Chan, Jodi Weidler, Roy Herbst, Michael Bates, David L. Rimm
Summary: This study investigates the use of PD-L1 mRNA level as a predictive biomarker for nonresponse to immune checkpoint inhibitors (ICIs) in lung cancer patients. The results show that high levels of PD-L1 and PD-L2 mRNA are associated with improved long-term benefit and overall survival, while low PD-L1 mRNA level has a high negative predictive value for absence of long-term benefit.
JOURNAL OF THORACIC ONCOLOGY
(2022)
Article
Chemistry, Analytical
Kazuyuki Nobusawa, Huan-Wen Han, Fumie Takei, Ting-Chieh Chu, Noriyasu Hashida, Ichiro Yamashita
Summary: We developed a new electrochemical impedimetric method for real-time detection of PCR. By adding a specific DNA intercalator and redox mediator, the progress of PCR reaction can be accurately monitored using impedance measurement. This simple method enables cost-effective and portable PCR devices.
ANALYTICAL CHEMISTRY
(2022)
Article
Infectious Diseases
Georgi Tomov, Nikola Stamenov, Deyan Neychev, Kiril Atliev
Summary: This study aimed to examine the possible association between tongue piercing and Candida spp. infection in healthy individuals. The results suggest that the main risk factors for tongue colonization with Candida spp. are gender, smoking, and improper hygiene, while tongue piercing is not a risk factor for colonization of Candida spp.
Article
Immunology
Danna Zhou, Shuangshuang Wang, Keli Yang, Xue Liu, Wei Liu, Rui Guo, Wan Liang, Fangyan Yuan, Zewen Liu, Ting Gao, Yong-xiang Tian
Summary: A new real-time nucleic acid sequence-based amplification (RT-NASBA) method was developed for rapid detection of Japanese encephalitis virus (JEV), showing high specificity and sensitivity.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
G. Papadakis, A. K. Pantazis, N. Fikas, S. Chatziioannidou, V Tsiakalou, K. Michaelidou, V Pogka, M. Megariti, M. Vardaki, K. Giarentis, J. Heaney, E. Nastouli, T. Karamitros, A. Mentis, A. Zafiropoulos, G. Sourvinos, S. Agelaki, E. Gizeli
Summary: A device for real-time colorimetric LAMP was developed, combining the accuracy of lab-based quantitative analysis with the simplicity of point-of-care testing, featuring rapid analysis, high quantification, crude sample-compatibility, low detection limit, smartphone operation, fast prototyping, and dye selection capabilities.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Ramtin Hadighi, Aliehsan Heidari, Parviz Fallah, Hossein Keshavarz, Ziba Tavakoli, Sholeh Mansouri, Mehrdokht Sadrkhanloo
Summary: This study evaluated changes in plasma miRNA markers of Plasmodium vivax in patients with malaria in Chabahar, Iran. The results showed that the expression of miR-223, miR-145, and miR-155 was significantly increased in patients with P. vivax compared to healthy individuals, suggesting that these miRNAs may serve as biomarkers for P. vivax malaria assessment.
