Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 174, Issue 1, Pages 136-143Publisher
ELSEVIER SCIENCE INC
DOI: 10.2353/ajpath.2009.080588
Keywords
-
Categories
Funding
- National Cancer Institute, SPORE in Cervical Cancer [P50 CA098252, CA118790]
- NATIONAL CANCER INSTITUTE [R01CA118790, P50CA098252] Funding Source: NIH RePORTER
Ask authors/readers for more resources
The expression pattern of human papillomavirus (HPV) capsid antigen L2 is poorly described, and the significance of its localization with both promyelocytic leukemia protein (PML) and Daxx in a sub-nuclear domain, nuclear domain 10 (ND-10), when ectopically expressed in tissue culture cells is controversial. To address whether ND-10 localization of L2 occurs in natural cervical lesions, we used a HPV16 and HPV18 U-specific monoclonal antibody (RG-1), in addition to rabbit antiserum to HPV6 L2, to localize L2. Immunohistochemical staining with RG-1 produced diffuse staining in the nuclei of some cells located within the superficial epithelial layers in eight of nine cases of HPV16/18(+) cervical intraepithelial neoplasia grade 1 (CIN1); however, no staining was observed in HPV16/18(+) high-grade CIN (0 of 8 cases), normal cervical epithelium (0 of 20 cases), cervical squamous cell carcinoma (0 of 102 cases), adenocarcinoma (0 of 51 cases), or adenosquamous carcinoma (0 of 6 cases). HPV16/18(+) cervical lesions that express L2 exhibit higher HPV16/18 genome copies per cell compared with those that do not positively stain with RG-1 (P = 0.04). RG-1 staining of HeLa cells transfected with L2 expression constructs was frequently concentrated in the ND-10, particularly in cells expressing high levels of L2, and co-localized with the cellular markers of ND-10, PML, and Daxx. In contrast, L2 was primarily diffuse within the nucleus and distinct from ND-10 as defined by PML immunoflurescent staining in CIN lesions, condylomata, and HPV16-transduced organotypic cultures. (Am J Pathol 2009, 174:136-143; DOI. 10.2353/ajpath.2009.080588)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available