Journal
AMERICAN JOURNAL OF PATHOLOGY
Volume 175, Issue 1, Pages 171-189Publisher
ELSEVIER SCIENCE INC
DOI: 10.2353/ajpath.2009.080769
Keywords
-
Categories
Funding
- Swedish Cancer Foundation
- Swedish Medical research Council
- Gustav V 80 Year Foundation
- Georg Wally Foundation
- Clas Groschinsky Foundation
- Swedish Society of Physicians
- UAS Cancer Foundation
- Mary, Ake och Hans Lindells Foundation
- Ake Wiberg Foundation
- Lions Cancer Foundation
- Agnes och Mac Rudbergs Foundation
- Pediatric Oncology Foundation
- Hans Jeanssons Foundatio
- NIH [RO1 CA101046]
Ask authors/readers for more resources
How activation of a specific growth factor receptor selectively results in either cell proliferation or cytoskeletal reorganization is of central importance to the field of pathophysiology. In this study, we report on a novel mechanism that explains how this process is accomplished. Our current investigation demonstrates that soluble platelet derived growth factor(PDGF)-beta activates a cohort of PDGF-beta receptors primarily confined to the lipid raft component of the cell membrane, specifically caveolae. in contrast, cell-bound PDGF-BB delivered via cell-cell contact results in activation and the subsequent up-regulation of a cohort of PDGFB-receptors primarily confined to the non-lipid raft component of the cell membrane. individual activation of these two receptor cohorts results in distinct biological endpoints, cytoskeletal reorganization or cell proliferation. Mechanistically, our evidence suggests that PDGF-BB-bearing cells preferentially stimulate the non-lipid raft receptor cohort through interleukin 1 beta-mediated inhibition of the lipid raft cohort of receptors, leaving the non-raft receptor cohort operational and preferentially stimulated. In human skin injected with PDGF-BB and in tissue reparative processes PDGF beta-receptors colocalize with the caveolae/lipid raft marker caveolin-1. In contrast, in human skin injected with PDGF-BB-bearing tumor cells and in colorectal adenocarcinoma, activated PDGF P-receptors do not colocalize with caveolin-1. Thus, growth factor receptors are segregated into specific cell membrane compartments that are preferentially activated through different mechanisms of ligand delivery, resulting in distinct biological endpoints. (Am J Pathol 2009, 175:171-189; DOI: 10.2353/ajpath.2009.080769)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available