Article
Genetics & Heredity
Doctor B. Sibandze, Alexander Kay, Viola Dreyer, Welile Sikhondze, Qiniso Dlamini, Andrew DiNardo, Godwin Mtetwa, Bhekumusa Lukhele, Debrah Vambe, Christoph Lange, Muyalo Glenn Dlamini, Tara Ness, Rojelio Mejia, Barbara Kalsdorf, Jan Heyckendorf, Martin Kuhns, Florian P. Maurer, Sindisiwe Dlamini, Gugu Maphalala, Stefan Niemann, Anna Mandalakas
Summary: Stool is an important diagnostic specimen for tuberculosis, especially in populations unable to provide sputum. However, conventional culture methods perform poorly in isolating M. tuberculosis complex strains from stool samples, limiting drug resistance testing. This study demonstrates that targeted next-generation sequencing (tNGS) can effectively detect drug resistance mutations from stool specimens, providing critical diagnostic information for tuberculosis patients who struggle to produce sputum samples.
Article
Multidisciplinary Sciences
Menen Tsegaw, Bezawit Mulat, Kegnie Shitu
Summary: This study aimed to assess safe child stool disposal practice and associated factors among mothers with children aged under-two years in Gambia. The prevalence of safe stool disposal was low in Gambia. Age of the mother, age of the child, region, wealth index, media exposure, and occupational status of the mother were significantly associated with safe child stool disposal.
Article
Multidisciplinary Sciences
Biniyam Sahiledengle, Zinash Teferu, Yohannes Tekalegn, Tadesse Awoke, Demisu Zenbaba, Kebebe Bekele, Abdi Tesemma, Fikadu Seyoum, Demelash Woldeyohannes
Summary: The study revealed that unsafe child stool disposal is spatially clustered in Ethiopia, with factors such as community poverty level, poor sanitation facilities, and the age of infants being significant contributors. Efforts to address unsafe child stool disposal should consider targeting households with higher poverty levels and improving sanitation facilities, particularly for households with young children.
Article
Biochemistry & Molecular Biology
Alessandra Frau, Lauren Lett, Rachael Slater, Gregory R. Young, Christopher J. Stewart, Janet Berrington, David M. Hughes, Nicholas Embleton, Chris Probert
Summary: The fecal metabolome in early life of pre-term babies was studied through analysis of stool samples using VOCs analysis. The metabolome shifted significantly 5 days after birth, coinciding with the establishment of enteral feeding and transition from meconium to feces. Prior to this shift, aldehydes and acetic acid dominated the metabolome, with a rise in branched-chain fatty acids observed. Additionally, caesarean delivery showed a modest association with molecules of fungal origin.
Article
Microbiology
Seung Yong Shin, Sounkou Kim, Ji Won Choi, Sang-Bum Kang, Tae Oh Kim, Geom Seog Seo, Jae Myung Cha, Jaeyoung Chun, Yunho Jung, Jong Pil Im, Ki Bae Bang, Chang Hwan Choi, Soo-Kyung Park, Dong Il Park
Summary: This study revealed common and unique microbiome patterns in saliva, intestinal tissue biopsy, and stool samples from patients with Crohn's disease. The microbial community in saliva was significantly different from that in tissue and stool. Prevotella and Streptococcus were common genera in saliva, tissue, and stool microbiomes.
Article
Infectious Diseases
Felix Bosch, Marta S. Palmeirim, Said M. Ali, Shaali M. Ame, Jan Hattendorf, Jennifer Keiser
Summary: This study aimed to investigate the impact of storage time, temperature, and stirring on fecal egg counts detectable by the Kato-Katz technique. Results showed that hookworm eggs rapidly decreased at room temperature, but could be preserved for about 110 minutes in a refrigerator. There was no significant trend observed for Ascaris lumbricoides and Trichuris trichiura fecal egg counts over time and storage temperature.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Leon Deutsch, Blaz Stres
Summary: This study introduced a minimal pressure approach (MP) to measure stool material crosslinking, finding that MP values increased exponentially with higher consistency, enabling stratification of samples exhibiting mixed BSS classes. The results provide insights into the relationship between stool hardness and human health, suggesting that the MP approach could be useful for future microbiome and metabolome studies.
Article
Microbiology
Vincenzo Di Pilato, Fabio Morecchiato, Cosmeri Rizzato, Gianluca Quaranta, Roberta Fais, Claudia Gandolfo, Alberto Antonelli, Maria Grazia Cusi, Mauro Pistello, Gian Maria Rossolini, Maurizio Sanguinetti, Antonella Lupetti, Luca Masucci
Summary: This study evaluated the performance of two commercially available assays for the detection of SARS-CoV-2 RNA in stool. The results showed that both assays were able to reliably detect SARS-CoV-2 with high reproducibility and accuracy. This suggests the potential use of these methods for the screening of FMT donors.
Article
Biochemical Research Methods
Magdalena Krausova, Kolawole I. Ayeni, Lukas Wisgrill, Chibundu N. Ezekiel, Dominik Braun, Benedikt Warth
Summary: Infants are sensitive to negative effects caused by food contaminants such as mycotoxins, but methods assessing mycotoxin exposure in infant stool are lacking. This study presents a novel LC-MS/MS assay for detecting multiple mycotoxins, validated in Austrian and Nigerian infant stool samples. Nigerian samples showed widespread mycotoxin contamination, while no mycotoxins were detected in Austrian samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Takeshi Hano, Mana Ito, Katsutoshi Ito, Motoharu Uchida
Summary: This study aimed to evaluate the impact of phenanthrene on fish health by analyzing alterations in fecal characteristics. Results showed that Phe exposure led to physiological stress markers increase in serum and liver, abnormal fecal appearance, and changes in fecal characteristics, including cholesterol and putrescine metabolism. The study also found that microbial community was altered by Phe exposure, with an increase in Phe-degrading bacteria such as Pseudomonas.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Physical
Il Son Khan, Luis Garzon-Tovar, Toni Grell, Genrikh Shterk, Jose Cerrillo, Tuiana Shoinkhorova, Juan C. Navarro, Faisal Alahmadi, Angel Sousa, Anastasiya Bavykina, Daria Poloneeva, Mustafa Caglayan, Stephanie Terruzzi, Javier Ruiz-Martinez, Nikolay Kosinov, Valentina Colombo, Jorge Gascon
Summary: The use of metal-organic frameworks (MOFs) as precursors for heterogeneous catalysts has attracted much attention. Steaming during the controlled decomposition of MOFs leads to the formation of smaller, well-dispersed metal nanoparticles, resulting in more efficient catalysts. This study demonstrates the benefits of steam addition, including improved phase composition control and catalyst activity, for CO2 hydrogenation.
Article
Chemistry, Organic
Guodong Sun, Zhuofei Deng, Zhonghua Luo, Zhongqing Wang, Jiancun Zhang
Summary: A highly efficient organocatalytic asymmetric synthesis of axially chiral biaryl phosphonates via CPA-catalyzed asymmetric arylations has been reported, providing excellent yields and enantioselectivities. The reaction can be conducted at a gram scale with low catalyst loading, offering a green and ready access to chiral biaryl monophosphorus ligands. Furthermore, compound 4ca was successfully converted to novel chiral biaryl monophosphorus ligands 7a, 7b, and 8 with high enantioselectivities in three steps.
Article
Endocrinology & Metabolism
Maria Sanchez-Campillo, Maria Teresa Pastor-Fajardo, Maria Sabater-Molina, Maria Jose Lopez-Andreo, Elvira Larque
Summary: This study investigates the critical steps of non-invasive methods for analyzing human gut cell gene expression in stool samples by examining prokaryote contamination in each step of different procedures and the influence of fecal sampling collection process. The results show that the least bacterial contamination is found when using RNA amplified with oligo dT from the GeneChip 3' IVT Pico Reagent Kit or using RNA purified by both Oligotex (R) + oligo dT.
ANNALS OF NUTRITION AND METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Mobin Makhmalbaf, Seyed Masoud Hosseini, Hamid Asadzadeh Aghdaei, Mahsa Saeedi Niasar, Shahrzad Shoraka, Abbas Yadegar, Shaghayegh Baradaran Ghavami, Shabnam Shahrokh, Mohammadreza Moshari, Habib Malekpour, Mohammad Reza Zali, Seyed Reza Mohebbi
Summary: This study investigated the presence and degree of SARS-CoV-2 genome in stool and plasma samples from 191 Iranian COVID-19 patients, and examined the relationship between these results and disease severity. The study found no correlation between the presence of SARS-CoV-2 genome in fecal and plasma samples of Covid-19 patients and the severity of illness.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, General & Internal
Ren-Jie Gong, Can-Xia Xu, Huan Li, Xiao-Ming Liu
Summary: The meta-analysis evaluated the feasibility of PCR-based tests for detecting H. pylori clarithromycin resistance in stool samples, showing high diagnostic accuracy. PCR analysis of stool samples had a high sensitivity of 0.91 and specificity of 0.97 for detecting clarithromycin resistance, with a diagnostic odds ratio of 347.68. Sub-analyses revealed significant differences in various factors, but no publication bias was found.
WORLD JOURNAL OF CLINICAL CASES
(2021)
