Article
Chemistry, Analytical
So Tanabe, Satohiro Itagaki, Kyohei Matsui, Shigeki Nishii, Yojiro Yamamoto, Yasuhiro Sadanaga, Hiroshi Shiigi
Summary: This paper presents a simple strategy for bacterial identification using the optical properties of polymer-coated metal nanoparticles. The study demonstrates that nanohybrid structures (NHs) can be used as optical markers based on their strong scattered light. The color of the scattered light can identify different bacterial species, making it a useful development for safety risk assessments and environmental surveys.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Biomaterials
Xiaoqian Jiang, Xinxin Xu, Hua Kuang, Liqiang Liu, Liguang Xu, Aihua Qu, Chuanlai Xu
Summary: In this study, a highly-sensitive and specific monoclonal antibody (mAb) 4C5 was obtained through coupling a carboxyl group introduced at the hydroxyl or carbon positions of tacrolimus with carrier protein. A colloidal gold-based immunochromatographic strip (CG-ICS) was established based on mAb 4C5 for rapid and accurate monitoring of tacrolimus in human whole blood. The CG-ICS showed a visual limit of detection (vLOD) and cut-off value of 1.0 and 20.0 ng mL(-1) for qualitative detection, and a LOD of 0.16 ng mL(-1) and linear range of 0.48-7.57 ng mL(-1) for quantitative detection. The results of CG-ICS were consistent with LC-MS/MS analysis for real positive samples, indicating its suitability for clinical monitoring of tacrolimus.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Tao Dong, Ran Yin, Qingcai Yu, Wanwei Qiu, Kun Li, Lisheng Qian, He Li, Bing Shen, Guodong Liu
Summary: The study presents a highly sensitive approach for detecting microRNA-21 (miR-21) in cancer cells and human serum using Au@Si nanocomposite labeled lateral flow assay. After systematic optimization, the method can detect a minimum concentration of 1.0 pM miR-21, which is 60 times lower than that of the GNP-based lateral flow assay.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Multidisciplinary
Marcelino Varona, Jared L. Anderson
Summary: Detection of mutations and single-nucleotide polymorphisms is crucial for diagnostic applications, and Loop-mediated isothermal amplification (LAMP) is a powerful tool for this purpose. However, traditional LAMP lacks the ability to distinguish single-nucleotide differences within target sequences. Various approaches have been developed to address this limitation, including primer design modifications and probe-based detection methods.
Review
Pharmacology & Pharmacy
Mariana R. Botton, Michelle Whirl-Carrillo, Andria L. Del Tredici, Katrin Sangkuhl, Larisa H. Cavallari, Jose A. G. Agundez, Jorge Duconge, Ming Ta Michael Lee, Erica L. Woodahl, Karla Claudio-Campos, Ann K. Daly, Teri E. Klein, Victoria M. Pratt, Stuart A. Scott, Andrea Gaedigk
Summary: This article introduces the star (*) allele nomenclature for the polymorphic human CYP2C19 gene catalogued by PharmVar, and highlights the impact of CYP2C19 genetic variation on drug metabolism, as well as how haplotype information compiled by PharmVar is utilized by CPIC.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Chemistry, Analytical
Fan He, Jinyi Yang, Tingting Zou, Zhenlin Xu, Yuanxin Tian, Wenjia Sun, Hong Wang, Yuanming Sun, Hongtao Lei, Zijian Chen, Juewen Liu, Xuecai Tan, Yudong Shen
Summary: A gold nanoparticle-based immunochromatographic assay was developed for simultaneous detection of sildenafil and its analogues, with qualitative and quantitative analysis capabilities in a rapid manner.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Shraddha Rahi, Vikram Lanjekar, Vandana Ghormade
Summary: In this study, a rapid and user-friendly dot-blot assay for the detection of ochratoxin A was developed using peptide conjugated gold nanoparticles as a detection agent. The assay exhibited high binding affinity towards ochratoxin A and had a low limit of detection. The dot-blot assay showed comparable results with the conventional high performance liquid chromatography in the detection of ochratoxin A in wheat samples. This portable and easy-to-use assay has the potential for application at different stages of food production and distribution to ensure safe and healthy food.
Review
Pharmacology & Pharmacy
Mariana R. Botton, Michelle Whirl-Carrillo, Andria L. Del Tredici, Katrin Sangkuhl, Larisa H. Cavallari, Jose A. G. Agundez, Jorge Duconge, Ming Ta Michael Lee, Erica L. Woodahl, Karla Claudio-Campos, Ann K. Daly, Teri E. Klein, Victoria M. Pratt, Stuart A. Scott, Andrea Gaedigk
Summary: Genetic variation in the CYP2C19 gene impacts the metabolism of many drugs and is associated with efficacy and safety issues for several commonly prescribed medications. The PharmVar catalogued haplotype information is utilized by the Pharmacogenomics Knowledgebase and CPIC.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Engineering, Environmental
Lu Zeng, Xinxin Xu, Lingling Guo, Zhongxing Wang, Hongliu Ding, Shanshan Song, Liguang Xu, Hua Kuang, Liqiang Liu, Chuanlai Xu
Summary: A monoclonal antibody against histamine was prepared with high sensitivity for rapid detection in fish samples, which was confirmed to complete the testing process within 15 minutes and produce comparable results with liquid chromatography-mass spectrometry and commercial test strips.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Pharmacology & Pharmacy
Zhen Yang, Yunqian Xie, Daya Zhang, Yan Zou, Ximei Li, Runxiang Chen, Xiaodong Zhang, Shiju Chen, Feihu Bai
Summary: Regional differences in CYP2C19*17 distribution were observed in Ningxia, with a higher frequency in the Hui population compared to the Han population. However, no significant relationship was found between CYP2C19 gene polymorphism and susceptibility to H. pylori infection.
PHARMACOLOGICAL REPORTS
(2023)
Article
Spectroscopy
Taehyeong Kim, Hyeongjin Jeon, Jung-Rok Lee, Dokyoon Kim
Summary: Researchers have reported a strategy for sensitive detection of dipicolinic acid (DPA) assisted by magnetic separation. By using poly(acrylic acid)-coated iron oxide nanoparticles as a magnetic carrier, only a necessary amount of Tb3+ ions are delivered to DPA in a sample solution, allowing for accurate photoluminescence measurement of the Tb3+-DPA complex with minimal background noise.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2024)
Article
Biotechnology & Applied Microbiology
Hyung-Mo Kim, Jaehi Kim, Jaehyun An, Sungje Bock, Xuan-Hung Pham, Kim-Hung Huynh, Yoonsik Choi, Eunil Hahm, Hobeom Song, Jung-Won Kim, Won-Yeop Rho, Dae Hong Jeong, Ho-Young Lee, Sangchul Lee, Bong-Hyun Jun
Summary: A semiquantitative LFIA platform using Au-Ag nanoparticles assembled on silica NPs as signal reporters was developed for PSA detection in blood. The color intensity of the test line on the test strip in LFIA varied depending on the PSA concentration, demonstrating high reproducibility and stability. This signal reporter showed reliability for PSA detection, with potential applications in clinical sample analysis.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Xhensila Shkembi, Mary Luz Botero, Vasso Skouridou, Miriam Jauset-Rubio, Marketa Svobodova, Pablo Ballester, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Abdulrahman O. Alyoubi, Ciara K. O. Sullivan
Summary: The illicit use of AAS as performance-enhancing drugs poses a threat to both competitive sports and public health. Current detection methods are limited to laboratory settings and cannot be performed on-site. However, the development of aptamer technology offers the potential for simple and cost-effective on-site analysis. In this study, a label-free colorimetric assay was developed using aptamers to detect the abuse of AAS.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Chemistry, Analytical
Kieu The Loan Trinh, Hoang Dang Khoa Do, Nae Yoon Lee
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has triggered a demand for accurate and cost-effective diagnostic tests for COVID-19 and other viral infections. Traditional virus screening methods have limitations in terms of equipment, personnel, and analysis time. Microfluidic-based biosensors, integrating microfluidic and biosensor technologies, offer a platform for sample preparation and simultaneous detection with high sensitivity, accuracy, portability, and real-time results. This review presents recent advances in microfluidic-based biosensors for virus detection, categorizing them into immunoassays and molecular assays, and analyzes available commercial sensing tests.
Article
Chemistry, Applied
Yiyi Chen, Haixi Lu, Honghui Shi, Junli Zhu, Haifeng Wang
Summary: A novel improved immunochromatographic assay using up-conversion nanoparticles was developed for the detection of zearalenone. The synthesized Lu3+-doped UCNPs showed optimal optical properties. The developed IUCNPs-ICA had a detection time of 10 min and a limit of detection of 0.1 μg/L.
Article
Pathology
Blake W. Buchan, Katherine M. Riebe, Markus Timke, Markus Kostrzewa, Nathan A. Ledeboer
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2014)
Review
Microbiology
Blake W. Buchan, Nathan A. Ledeboer
CLINICAL MICROBIOLOGY REVIEWS
(2014)
Article
Immunology
Tyler K. Ulland, Ann M. Janowski, Blake W. Buchan, Matthew Faron, Suzanne L. Cassel, Bradley D. Jones, Fayyaz S. Sutterwala
INFECTION AND IMMUNITY
(2013)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Katherine M. Riebe, Lauren N. Parsons, Stacy Gnacinski, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2012)
Article
Microbiology
Blake W. Buchan, Katherine M. Riebe, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2012)
Article
Microbiology
Blake W. Buchan, Wendy J. Olson, Michael Pezewski, Mario J. Marcon, Thomas Novicki, Timothy S. Uphoff, Lakshmi Chandramohan, Paula Revell, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Carol L. Young, Duane W. Newton, Connie Brenke, Linda Lapsley, Paul A. Granato, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Donna Mayne, Joel E. Mortensen, Tami-Lea A. Mackey, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Article
Microbiology
Karen C. Carroll, Blake W. Buchan, Sokha Tan, Paul D. Stamper, Katherine M. Riebe, Preeti Pancholi, Cheryl Kelly, Arundhati Rao, Robert Fader, Robert Cavagnolo, Wendy Watson, Richard V. Goering, Ernest A. Trevino, Alice S. Weissfeld, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Review
Microbiology
Blake W. Buchan, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2013)
Review
Microbiology
Neil W. Anderson, Blake W. Buchan, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Microbiology
Blake W. Buchan, Wendy J. Olson, Tami-Lea A. Mackey, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Microbiology
Eric T. Beck, Blake W. Buchan, Katherine M. Riebe, Brenda R. Alkins, Preeti Pancholi, Paul A. Granato, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Microbiology
Neil W. Anderson, Blake W. Buchan, Nathan A. Ledeboer
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Medicine, General & Internal
Blake W. Buchan, Christine C. Ginocchio, Ryhana Manii, Robert Cavagnolo, Preeti Pancholi, Lettie Swyers, Richard B. Thomson, Christopher Anderson, Karen Kaul, Nathan A. Ledeboer