Article
Pathology
Natalie M. Rizzo, Lynette M. Sholl, Chia-Sui Kao, Kristine M. Cornejo, Ankur R. Sangoi, Michelle S. Hirsch, Katrina Collins, Jennifer B. Gordetsky, Fabiola A. Reyes Curcio, Christopher D. M. Fletcher, Thomas M. Ulbright, Andres M. Acosta
Summary: This study analyzed a series of non-metastasizing and metastasizing SCTs using next-generation DNA sequencing and found that non-metastasizing SCTs were mainly driven by CTNNB1 and APC gene variants, while metastasizing SCTs were mainly caused by variants in TP53, MDM2, CDKN2A/CDKN2B, and TERT genes.
Article
Pediatrics
Giorgio Persano, Alessandro Crocoli, Maria Debora De Pasquale, Raffaele Cozza, Rita Alaggio, Francesca Diomedi Camassei, Federico Beati, Pierluigi Di Paolo, Cristina Martucci, Alessandro Inserra
Summary: Burned-out testicular tumors are a distinct clinical entity characterized by nodal or visceral metastases as clinical manifestations without a clinically or radiologically evident primary testicular lesion. These tumors are associated with systemic symptoms, higher levels of tumor markers, increased treatment-related toxicity, and poorer outcomes compared to primary testicular tumors. Further research is needed to better understand burned-out testicular tumors and develop more effective therapeutic strategies.
FRONTIERS IN PEDIATRICS
(2021)
Article
Medicine, General & Internal
Richard Colling, Andrew Protheroe, Mark Sullivan, Ruth Macpherson, Mark Tuthill, Jacqueline Redgwell, Zoe Traill, Angus Molyneux, Elizabeth Johnson, Niveen Abdullah, Andrea Taibi, Nikki Mercer, Harry R. Haynes, Anthony Sackville, Judith Craft, Joao Reis, Gabrielle Rees, Maria Soares, Ian S. D. Roberts, Darrin Siiankoski, Helen Hemsworth, Derek Roskell, Sharon Roberts-Gant, Kieron White, Jens Rittscher, Jim Davies, Lisa Browning, Clare Verrill
Summary: This article shares the experience of creating a digital pathology service for germ cell tumors, highlighting both the benefits and challenges of digitization. The survey results indicate that team engagement is crucial for successful clinical transformation.
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Duygu Has Simsek, Caner Civan, Meltem Ekenel, Serkan Kuyumcu, Yasemin Sanli
Summary: Overexpression of prostate-specific membrane antigen (PSMA) is demonstrated in various tumors, and radionuclide therapies directed at PSMA might be effective in treating PSMA-avid malignancies. A case study of a 46-year-old male with progressive metastatic testicular mixed germ cell tumor showed mixed response in Ga-68-PSMA PET/CT after Lu-177-PSMA therapy, suggesting the need for further optimization of treatment strategies.
CLINICAL NUCLEAR MEDICINE
(2021)
Article
Pathology
Jeffrey A. Stump, Andres M. Acosta, Rumeal D. Whaley, Liang Cheng, Andrew M. Fang, Soroush Rais-Bahrami, Jennifer B. Gordetsky
Summary: Treatment for testicular germ cell tumors (GCTs) typically involves orchiectomy and chemotherapy, with rare cases requiring neoadjuvant chemotherapy prior to surgery. Research shows that the pathology of orchiectomy often differs from metastatic tissue in patients undergoing neoadjuvant chemotherapy, and those with no remaining testicular disease have a better prognosis.
Review
Urology & Nephrology
Muhannad Alsyouf, Siamak Daneshmand
Summary: Testicular germ cell tumors (GCTs) are the most common malignancy among young males. The majority of patients present with early stages of the disease that are highly curable. Primary retroperitoneal lymph node dissection (RPLND) has been shown to be effective for low-volume metastatic GCT, providing an alternative to systemic chemotherapy.
EUROPEAN UROLOGY FOCUS
(2023)
Review
Oncology
Jane Belfield, Charlotte Findlay-Line
Summary: This article provides a detailed description of the use of ultrasound in germ cell tumours and offers guidance on performing an ultrasound examination. It also includes the classification of germ cell tumours and describes the key features of different types of tumours. The challenges and pitfalls of ultrasound are discussed, as well as other applications of ultrasound in this patient group.
Review
Biochemistry & Molecular Biology
Massimo De Felici, Francesca Gioia Klinger, Federica Campolo, Carmela Rita Balistreri, Marco Barchi, Susanna Dolci
Summary: In the human embryo, the genetic program responsible for germ cell specification involves a balance between epigenetic and transcriptional mechanisms, resulting in a unique cell population that can exhibit both germness and pluripotency features. Germ cell tumors, originating from fetal or neonatal germ cells, maintain this balance and can display a wide range of phenotypes with either pluripotent or germness characteristics. This review highlights the similarities in transcriptional and epigenetic programs between human primordial germ cells and germ cell tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Liza O'Donnell, Laura F. Dagley, Michael Curley, Annalucia Darbey, Peter J. O'Shaughnessy, Thorsten Diemer, Adrian Pilatz, Daniela Fietz, Peter G. Stanton, Lee B. Smith, Diane Rebourcet
Summary: In this study, Sertoli cell-enriched proteins in testicular interstitial fluid (TIF) were identified and found to play potential roles in regulating somatic cell-cell communication and testis function.
Article
Developmental Biology
Peter K. Nicholls, David C. Page
Summary: Germ cells commit to gametogenesis after colonizing the gonads, and germ cell tumors may originate from cells that failed to undergo germ cell determination. The diversity of cell types in these tumors reflects the developmental potential of migratory PGCs.
Article
Oncology
Nianyu Xue, Guoyao Wang, Shengmin Zhang, Yijun Lu
Summary: Unlike younger patients, seminoma is not common in patients with primary testicular tumors over the age of 50. The diagnostic value of conventional ultrasonography and contrast-enhanced ultrasonography (CEUS) in these patients was compared. The CEUS method showed higher accuracy in distinguishing between germ cell tumors and non-germ cell tumors.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xingdong Wang, Jie Pei, Lin Xiong, Shaoke Guo, Mengli Cao, Yandong Kang, Ziqiang Ding, Yongfu La, Chunnian Liang, Ping Yan, Xian Guo
Summary: This study conducted a comprehensive and unbiased single-cell transcriptomic analysis of spermatogenesis in sexually mature 4-year-old yaks using scRNA-seq. The analysis revealed the presence of six somatic cell types and various germ cells in yak testis. Functional enrichment analysis showed that highly expressed genes in yak testicular somatic cells were significantly enriched in cAMP signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, and ECM receptor interactions. Immunohistochemical techniques identified candidate marker genes for spermatogonial stem cells and Sertoli cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pathology
Charles C. Guo, Bogdan Czerniak
Summary: The development of somatic-type malignancy in testicular germ cell tumor is a major challenge in the diagnosis and treatment of testicular cancer. Most somatic-type malignancies are derived from teratoma and occur more frequently in metastases. Different histologic types can be observed, and their prognosis varies. Timely surgical resection is an effective treatment for most patients.
Article
Oncology
Sounak Gupta, Lynette M. Sholl, Yiying Yang, Adeboye O. Osunkoya, Jennifer B. Gordetsky, Kristine M. Cornejo, Kvetoslava Michalova, Fiona Maclean, Eugenia Dvindenko, Matija Snuderl, Michelle S. Hirsch, William J. Anderson, Ross A. Rowsey, Rafael E. Jimenez, John C. Cheville, Peter M. Sadow, Maurizio Colecchia, Costantino Ricci, Thomas M. Ulbright, Daniel M. Berney, Andres Martin Acosta
Summary: This study analyzed the genomic and epigenomic features of spermatocytic tumors (STs). The results showed that STs can be divided into two genomic subgroups, one with global ploidy shifts and the other with diploid genomes and RAS/RAF hotspot mutations. Aggressive behavior was associated with relative gains of chromosome 12p and TP53 mutations.
JOURNAL OF PATHOLOGY
(2023)
Editorial Material
Radiology, Nuclear Medicine & Medical Imaging
Yu-Chin Wu, Chia-Hung Kao, Shung-Shung Sun, Te-Chun Hsieh
Summary: We report a case of a 20-year-old male diagnosed with metastatic embryonal carcinoma, which was subsequently confirmed as burned-out testicular mixed germ cell tumor.
CLINICAL NUCLEAR MEDICINE
(2022)
