Journal
AMERICAN JOURNAL OF CHINESE MEDICINE
Volume 41, Issue 1, Pages 221-229Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S0192415X1350016X
Keywords
RDN Injection; Bioactive Components; Macrophage Extraction; UPLC-MS
Funding
- China National Key Basic Research Program [2010CB735604]
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In this paper, a useful method for screening and analyzing the potential bioactive components from Reduning Injection was developed using macrophage cell extraction and ultra-high performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on macrophage cell damage induced by LPS in vitro were also investigated. The results showed that chlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid significantly inhibited the prostaglandin E-2 (PGE(2)) release and reversed the interleukin-6 (IL-6) secretion of macrophages (p < 0: 05). These data indicated that the method of macrophage cell extraction coupled with UPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCM injection.
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