Journal
AMERICAN JOURNAL OF CHINESE MEDICINE
Volume 39, Issue 3, Pages 579-586Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S0192415X11009044
Keywords
Corydalis yanhusuo WT Wang Extract; Proliferation; Reactive Oxygen Species; Mitochondrial Membrane Potential; Cell Cycle Arrest
Funding
- Macao Science and Technology Development Fund [029/2007/A2]
- University of Macau [UL016/09-Y2/CMS/WYT01/ICMS]
- Fujian University of Traditional Chinese Medicine
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Corydalis yanhusuo W. T. Wang (YHS) is a traditional Chinese herb widely prescribed for promoting blood circulation, reinforcing vital energy and alleviating pain. Our previous studies showed that an ethanol extract of YHS inhibits metastasis of breast cancer cells in vitro. In the present study, the anti-proliferative effect of the extract was determined by MTT assay and the LDH release was measured with a commercial kit. Intracellular reactive oxygen species (ROS) production and mitochondrial membrane potential (Delta Psi m) were monitored by CM-H(2)DCF-DA and JC-1 staining, respectively. Cell cycle was analyzed with propidium iodide (PI) staining by flow cytometry and protein expressions were measured by Western blotting. The YHS extract significantly inhibited MCF-7 cell proliferation in a dose-dependent manner. Significant increase of ROS formation and decrease of Delta Psi m were observed. Furthermore, it induced MCF-7 cell cycle arrest at the G2/M phases. In addition, the p-cdc-2/cdc-2 protein expression ratio was increased while Rb and p21 protein expressions were decreased. The YHS extract inhibited MCF-7 proliferation by inducing G2/M cell cycle arrest, which might be mediated by inducing ROS formation, decreasing Delta Psi m and regulating cell cycle related protein expressions.
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