Article
Biochemistry & Molecular Biology
Lissuly Guadalupe Alvarez-Hilario, Eric Genaro Salmeron-Barcenas, Pedro Antonio Avila-Lopez, Georgina Hernandez-Montes, Elena Arechaga-Ocampo, Roberto Herrera-Goepfert, Jorge Albores-Saavedra, Maria del Carmen Manzano-Robleda, Hector Ivan Saldivar-Ceron, Sandra Paola Martinez-Frias, Maria Del Rocio Thompson-Bonilla, Miguel Vargas, Rosaura Hernandez-Rivas
Summary: Pancreatic ductal adenocarcinoma (PDAC) has a high fatality rate due to late detection. miRNAs can be used as noninvasive biomarkers for early diagnosis of PDAC, and their overexpression is associated with decreased survival rates in patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Antonio Di Maio, Anna Cioce, Silvia Achilli, Michel Thepaut, Corinne Vives, Franck Fieschi, Javier Rojo, Niels-C. Reichardt
Summary: In this study, glycodendron microarrays with defined valency were constructed on hydrophobic indium tin oxide coated glass slides using spotwise strain-promoted azide-alkyne cycloaddition (SPAAC). The immobilization of dendrons on the ITO surface allowed for the analysis of dendron surface density and SPAAC conversion rate. By manipulating dendron surface density, the study showed that carbohydrate-lectin interactions were exclusively dependent on the valency of the immobilized glycodendron.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Review
Allergy
Giuliana Ferrante, Amelia Licari, Salvatore Fasola, Gian Luigi Marseglia, Stefania La Grutta
Summary: Artificial intelligence shows great potential in healthcare, particularly in pediatric allergy research. AI methods can help differentiate the heterogeneity of allergic diseases among patients, improve data quality, and provide new perspectives for diagnostic frameworks.
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jian Shu, Xiameng Ren, Hongwei Cheng, Shiyi Wang, Lixin Yue, Xia Li, Mengqi Yin, Xiangqin Chen, Tiantian Zhang, Ziye Hui, Xiaojuan Bao, Wanghua Song, Hanjie Yu, Liuyi Dang, Chen Zhang, Jun Wang, Qi Zhao, Zheng Li
Summary: This study reveals the impact of benign lesions on salivary glycopatterns and cancer screening, and demonstrates that adding benign diseases to the control group improves the performance of the classifier.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Tiago Azenha Rama, Diana Martins, Nuno Gomes, Jorge Pinheiro, Ana Nogueira, Luis Delgado, Jose Luis Placido, Alice Coimbra
Summary: Mastocytosis is a rare and heterogeneous group of disorders characterized by expansion and accumulation of clonal mast cells, presenting mainly with cutaneous lesions and anaphylaxis. Due to its unusual features, the diagnosis of mastocytosis may be delayed for several years.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Allergy
Domingo Barber, Araceli Diaz-Perales, Maria M. Escribese, Jorg Kleine-Tebbe, Paolo M. Matricardi, Markus Ollert, Alexandra F. Santos, Joaquin Sastre
Summary: Progressive knowledge of allergenic structures and the application of molecular diagnosis have improved the accuracy of allergen immunotherapy decisions. Parallel to this, the establishment of regulations has impacted the development of allergen diagnostics and therapies.
Article
Allergy
Isabel J. Skypala, Hannah Hunter, Mamidipudi Thirumala Krishna, Helena Rey-Garcia, Stephen J. Till, George du Toit, Elizabeth Angier, Sarah Baker, Kostadin V. Stoenchev, David K. Luyt
Summary: Pollen food syndrome (PFS) is a common food allergy caused by the homology between pollen allergens and proteins in certain foods. Diagnosing PFS can usually be done based on clinical history, but additional tests may be needed in some cases. Management involves avoiding trigger foods, which can be challenging for individuals with other food allergies or those on a vegetarian/vegan diet. Currently, there is no effective treatment for PFS, but oral or sublingual immunotherapy may hold promise.
CLINICAL AND EXPERIMENTAL ALLERGY
(2022)
Review
Microbiology
Sandra Pfeiffer, Ines Swoboda
Summary: Fungal allergy is a global public health burden, but reliable diagnosis remains a challenge. The lack of high-quality standardized fungal extracts contributes to the underdiagnosis. The manufacturing processes for fungal extracts show the highest variability, and there is still much unknown about its impact on allergenicity. Further research, standardization, and guidelines are needed in the field of fungal allergology.
Article
Allergy
Lucy Duan, Alper Celik, Jennifer A. Hoang, Klara Schmidthaler, Delvin So, Xiaojun Yin, Christina M. Ditlof, Marta Ponce, Julia E. M. Upton, Jean-Soo Lee, Lisa Hung, Heimo Breiteneder, Chiara Palladino, Adelle R. Atkinson, Vy H. D. Kim, Alireza Berenjy, Maria Asper, David Hummel, Samantha Wong, Mara Alexanian-Farr, Ahuva Magder, Sharon R. Chinthrajah, Kaori Mukai, Mindy Tsai, Kari Nadeau, Stephen J. Galli, Arun K. Ramani, Zsolt Szepfalusi, Thomas Eiwegger
Summary: The basophil activation test (BAT) can effectively diagnose peanut and tree nut allergies, reducing the need for high-risk OFCs in multi-nut-sensitized children.
Review
Plant Sciences
Priyanga Suriyamoorthy, Alluru Madhuri, Srikanth Tangirala, Karunai Raj Michael, Vignesh Sivanandham, Ashish Rawson, Arunkumar Anandharaj
Summary: The pulp of banana fruit contains various beneficial bioactive compounds that enhance the immune system and prevent deadly diseases. However, bananas are also recognized as a common source of allergy, with 0.6% of the general population and even higher percentages among asthma or atopic dermatitis patients being allergic to bananas. Banana allergy occurs due to abnormal immune response to banana proteins, leading to symptoms ranging from oral allergy syndrome to life-threatening anaphylaxis. This review focuses on the pathogenesis, clinical features, diagnosis, and food processing methods to mitigate the allergenicity of banana fruit.
PLANT FOODS FOR HUMAN NUTRITION
(2022)
Review
Allergy
Jose-Maria Maesa, Agnieszka Dobrzynska, Elena Banos-Alvarez, Rebeca Isabel-Gomez, Juan-Antonio Blasco-Amaro
Summary: This systematic review aimed to assess the diagnostic accuracy of ImmunoCAP ISAC compared to oral food challenge. The study found that the specificity of ISAC components was generally higher than sensitivity, highlighting the need for higher quality information to determine the clinical utility of ISAC in diagnosing food allergies.
CLINICAL AND EXPERIMENTAL ALLERGY
(2021)
Article
Food Science & Technology
Roni Nugraha, Thimo Ruethers, Aya C. Taki, Elecia B. Johnston, Shaymaviswanathan Karnaneedi, Sandip D. Kamath, Andreas L. Lopata
Summary: This study investigates the IgE sensitisation profiles of patients with oyster allergy to raw and heated Pacific oyster extract. Tropomyosin is identified as the major allergen responsible for oyster allergy, and the study also reveals partial cross-reactivity and/or co-sensitisation between oyster, prawn, and dust mite allergens. This research emphasizes the importance of using recombinant tropomyosin in improved diagnostics and immunotherapy.
Article
Medical Laboratory Technology
Zhen Tang, Fan Zhang, Yuan Wang, Chen Zhang, Xia Li, Mengqi Yin, Jian Shu, Hanjie Yu, Xiawei Liu, Yonghong Guo, Zheng Li
Summary: This study demonstrated alterations in salivary glycopatterns in patients with HC and HCC, with significant differences in glycopatterns recognized by 22 lectins in the saliva of HC and HCC patients and healthy volunteers. The Random Forest algorithm showed the best predictive performance in constructing models for HC and HCC, with AUCs of 0.857 and 0.886 in the validation cohort, respectively.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Biochemical Research Methods
Huma Shehwana, Shwetha Kumar, James M. Melott, Mary A. Rohrdanz, Chris Wakefield, Zhenlin Ju, Doris R. Siwak, Yiling Lu, Bradley M. Broom, John N. Weinstein, Gordon B. Mills, Rehan Akbani
Summary: Reverse-Phase Protein Array (RPPA) is a platform for quantitatively measuring proteins in biological specimens. Converting raw data into normalized, analysis-ready data remains challenging. RPPA SPACE is an improved tool that enhances data quality and has simpler input requirements and higher flexibility.
Article
Biochemical Research Methods
Hsiu-Chung Tu, Yun Tang, Yi-Hua Lee, Fang-Ming Chen, Po-Chiao Lin
Summary: Breast cancer is a common malignancy with abnormal glycans. A synthetic boronic acid-disulfide (BASS) probe has been developed for glycoprotein recognition and labeling. The BASS-functionalized slide is a powerful platform for monitoring glycan pattern changes in human sera, particularly in breast cancer patients. This BASS-directed glycoprotein strategy shows promise for a rapid screening of clinical breast cancer samples and can be applied to other cancer prediagnoses.
BIOCONJUGATE CHEMISTRY
(2023)