Review
Environmental Sciences
Shang-Wu Shih, Jia-Jiun Yan, Ming-Yi Chou, Pung-Pung Hwang
Summary: The uptake of Na+ by teleosts from freshwater has been studied for the past 85 years. Different models have been proposed to explain the mechanisms behind Na+ uptake in teleost ionocytes, but there are still debates and unclear mechanisms. This mini-review summarizes the progress and regulatory mechanisms of Na+ uptake in teleosts, and discusses the challenges to the current models.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Environmental Sciences
Shu Mo, Yan Cui, Kehuan Sun, Haixia Wang, Xunqian Peng, Ling Ou, Xiaojun Lei, Mengtian Huang, Wenhui Mei, Ling Xin, Haibing He, Bojia Peng, Ya Tian, Panpan Wang, Xiaoyun Li, Ronghua Zhang, Xiaofeng Zhu
Summary: A high-salt diet can negatively impact renal function and bone metabolism by regulating ion channels and cytokines, resulting in decreased bone mineral density and bone loss.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Biochemistry & Molecular Biology
Oscar Brenes, Michael Pusch, Fernando Morales
Summary: Myotonia congenita is a hereditary muscle disease caused by mutations in the CLCN1 gene, leading to abnormal function of the chloride ion channel and resulting in muscle hyperexcitability and other symptoms. This review examines over 350 reported mutations and analyzes their effects on the structure and function of the chloride ion channel.
Article
Medicine, Research & Experimental
Jiezhu Feng, Zihan Peng, Lvfen Gao, Xiurou Yang, Zele Sun, Xiuying Hou, Enze Li, Linyan Zhu, Haifeng Yang
Summary: Over-expression of ClC-3 protein in PTX-resistant ovarian cancer cells promotes the combination of ClC-3 and beta-tubulin, reducing sensitivity to PTX.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Environmental Sciences
Chia-Hao Lin, Hung-Chi Chang, Sian-Tai Liu, Huei-Jyun Hu
Summary: Freshwater teleosts have defense mechanisms to maintain the homeostasis of body-fluid ion and acid-base balance. Vitamin D plays an important role in the regulation of Ca2+ uptake and acid secretion in these fish. The present study explored the effect of vitamin D on ionocyte proliferation and differentiation using zebrafish larvae as a model. The results showed that vitamin D treatment increased the number of ionocyte progenitors and mature ionocytes, suggesting a positive effect on ionocyte differentiation and stress adaptation in fish.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Environmental Sciences
Chia-Hao Lin, Sian-Tai Liu, Yu-Chun Wang, Yi-Ling Tsou, Huei-Jyun Hu
Summary: Maintenance of an acid-base balance is crucial for vertebrates, and fishes secrete more acid in response to acidity. This study using zebrafish as a model revealed the involvement of vitamin D in acid secretion in fishes.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Concetta Altamura, Elena Conte, Carmen Campanale, Paola Laghetti, Ilaria Saltarella, Giulia Maria Camerino, Paola Imbrici, Jean-Francois Desaphy
Summary: This study found that niflumic acid (NFA) can act as a pharmacological chaperone and has therapeutic effects on patients with specific ClC-1 channel mutations in myotonia congenita (MC). NFA can improve the expression of ClC-1 protein on the plasma membrane to restore normal chloride ion channel function.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Bing Zhang, Sensen Zhang, Maya M. Polovitskaya, Jingbo Yi, Binglu Ye, Ruochong Li, Xueying Huang, Jian Yin, Sebastian Neuens, Tom Balfroid, Julie Soblet, Daphne Vens, Alec Aeby, Xiaoling Li, Jinjin Cai, Yingcai Song, Yuanxi Li, Marco Tartaglia, Yang Li, Thomas J. Jentsch, Maojun Yang, Zhiqiang Liu
Summary: The study reveals that ClC-6 is an ion exchanger protein expressed in the nervous system and associated with severe neurological diseases. The researchers used cryo-EM structures and functional studies to uncover the structure, function, and regulation of ClC-6, as well as identifying hotspots for disease-causing mutations.
Article
Neurosciences
Maria Antonietta Coppola, Paola Gavazzo, Ilaria Zanardi, Abraham Tettey-Matey, Antonella Liantonio, Peying Fong, Michael Pusch
Summary: ClC-6 and ClC-7 are closely related intracellular Cl-/H+ antiporters that belong to the CLC family of channels and transporters. They play important roles in regulating ionic homeostasis in late endosomes and lysosomes. While the transport function of ClC-7 requires the association with the accessory protein Ostm1, ClC-6 does not. By studying their Cl- and pH-dependence, researchers have discovered unique characteristics and functional nuances of these proteins, as well as disease-associated mutants. The findings highlight the distinct roles of ClC-6 and ClC-7 in the context of their localization, and provide insights into the mechanisms underlying diseases associated with these proteins.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cell Biology
Oscar Brenes, Raffaella Barbieri, Melissa Vasquez, Rebeca Vindas-Smith, Jeffrey Roig, Adarli Romero, Gerardo del Valle, Luis Bermudez-Guzman, Sara Bertelli, Michael Pusch, Fernando Morales
Summary: Non-dystrophic myotonias are associated with loss-of-function mutations in the ClC-1 chloride channel or gain-of-function mutations in the Na(v)1.4 sodium channel. A family with members diagnosed with Thomsen's disease was found to have novel mutations in CLCN1 and SCN4A genes, leading to myotonia. The study highlights the importance of deep genetic analysis in accurate genetic counseling and patient management.
Article
Medicine, Research & Experimental
Meng-Hsuan Lin, Jen-Chi Chen, Xuejiao Tian, Chia-Ming Lee, I-Shing Yu, Yi-Fen Lo, Shinichi Uchida, Chou-Long Huang, Bi-Chang Chen, Chih-Jen Cheng
Summary: The study revealed that both ClC-Ka and ClC-Kb are broadly expressed and colocalized in perinatal mouse kidneys, with both participating in NaCl reabsorption in neonatal kidneys. Deletion of ClC-K2 causes tubular injury and salt wasting, while ClC-K1 and ClC-K2 together play a role in salt reabsorption in TAL and DCT in neonates.
Article
Marine & Freshwater Biology
Giun-Yi Hung, Po-Yen Chen, Jiun-Lin Horng, Li-Yih Lin
Summary: Environmental contamination by anticancer pharmaceuticals has been widely reported, especially their detrimental effects on zebrafish embryonic development and skin functions. Anticancer drug vincristine (VCR) exposure led to significant teratogenic effects and skin ionocytes reduction in zebrafish embryos. These findings highlight the potential developmental defects and impairment risks associated with acute exposure to VCR.
AQUATIC TOXICOLOGY
(2021)
Article
Endocrinology & Metabolism
Shuang Li, Helena Li, Zhengyi Wang, Cunming Duan
Summary: Stc1a plays a role in regulating calcium homeostasis and organismal survival by suppressing Trpv6 expression and inhibiting IGF signaling. Loss of Stc1a leads to increased Trpv6 expression in NaR cells and altered IGF signaling. Genetic or pharmacological inhibition of Trpv6 can alleviate phenotypes associated with Stc1a loss.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Environmental Sciences
Jenna Wiegand, Vanessa Cheng, Aalekhya Reddam, Sarah Avila-Barnard, David C. Volz
Summary: This study found that TPHP causes pericardial edema and a significant increase in ionocyte abundance in zebrafish embryos. Mannitol can block these effects, while knockdown of ATPase1a1.4 can only mitigate the increase in ionocyte abundance and ouabain can only enhance the occurrence of pericardial edema.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Dun Niu, Lanfang Li, Zhizhong Xie
Summary: ClC-3, a chloride antiporter, is a key mediator in various diseases, especially in the process of atherosclerosis. Studies have shown that ClC-3 may participate in atherosclerotic plaque formation through regulating inflammation-related signaling pathways, lipoprotein levels, and cell functions.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Yung-Che Tseng, Jia-Jiun Yan, Fumiya Furukawa, Pung-Pung Hwang
Article
Biochemistry & Molecular Biology
Min-Chen Wang, Yu-Chun Wang, Hui-Wen Peng, Jinn-Rong Hseu, Guan-Chung Wu, Ching-Fong Chang, Yung-Che Tseng
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
Pei-Chen Huang, Tzu-Yen Liu, Marian Y. Hu, Isabel Casties, Yung-Che Tseng
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Garett J. P. Allen, Pou-Long Kuan, Yung-Che Tseng, Pung-Pung Hwang, Alex R. Quijada-Rodriguez, Dirk Weihrauch
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Oki Hayasaka, Kazuhiko Anraku, Yuya Akamatsu, Yung-Che Tseng, Miguel Vazquez Archdale, Tomonari Kotani
Summary: A new analytical method was proposed for determining the response threshold in electroretinogram, showing quick and reliable objective assessment through template wave matching. ERG recordings in response to different stimuli showed similarities in waveform but differences in amplitude and time lag under varying intensity conditions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Physiology
Garett Joseph Patrick Allen, Min-Chen Wang, Yung-Che Tseng, Dirk Weihrauch
Summary: Emersion affects water-breathing animals’ ability to regulate respiratory gas exchange, acid-base balance, and nitrogenous waste excretion. Helice formosensis mitigates these effects by altering urine and branchial water, but prolonged emersion may result in overcompensatory metabolic alkalosis. During emersion, ammonia can be temporarily converted to alanine, indicating a unique adaptation mechanism in these crustaceans. Additionally, Helice formosensis can actively suppress desiccation by producing hyperosmotic urine.
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY
(2021)
Article
Biology
Joan Tang Xiao Joe, Yung-Che Tseng, Jen-Leih Wu, Ming-Wei Lu
Summary: The intestinal microbiota communities in grouper infected with pathogens show decreased microbial diversity and increased microbial richness. Pathogen-infected grouper have higher levels of harmful genera in their intestines, while beneficial genera predominate in the intestines of the control group. In addition, immune-related genes such as immunoglobulin T and proinflammatory cytokines show increased expression levels in the intestine after pathogen infection.
Review
Endocrinology & Metabolism
Ying-Jey Guh, Yung-Che Tseng, Yi-Ta Shao
Summary: Chronic underwater noise has a significant impact on fish physiology, including hormonal regulation, oxidative stress, and neurotransmitter regulation. Studies indicate that noise pollution could threaten fish populations by affecting their physiological and behavioral responses.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Shang-Wu Shih, Jia-Jiun Yan, Yi-Hsing Wang, Yi-Ling Tsou, Ling Chiu, Yung-Che Tseng, Ming-Yi Chou, Pung-Pung Hwang
Summary: In the study using medaka, Err gamma 2 was found to be a key player in ion regulation, controlling Na+/Cl- uptake by regulating ncc and/or nhe3 mRNA expression and ionocyte number, with regulatory actions subtly adjusted depending on internal and external ion concentrations. These findings provide new insights into the mechanism of actions of ERRs and enhance understanding of their roles in body fluid ionic homeostasis for adaptation to changing environments during vertebrate evolution.
JOURNAL OF ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Min-Chen Wang, Mao-Ting Hsu, Ching-Chun Lin, Shao-Chun Hsu, Ruo-Dong Chen, Jay-Ron Lee, Yi-Lin Chou, Hua-Pin Tseng, Fumiya Furukawa, Sheng-Ping L. Hwang, Pung-Pung Hwang, Yung-Che Tseng
Summary: This study found that tropical tilapia demonstrate adaptive trade-off mechanisms in response to thermal perturbations through transgenerational metabolic adjustments. However, global warming may lead to reduced thermal variation and lower energy deposits in tropical tilapia. Lack of cold exposure across multiple generations of fish may also decrease the native cold-tolerance traits of subtropical/tropical organisms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Evolutionary Biology
Wen-Chien Huang, Hong-Ming Chen, Yung-Che Tseng, Te-Yu Liao
Summary: This study examines phenovariant in three species pairs of sympatric morays from Taiwanese waters, revealing the natural hybridization of marine eel taxa for the first time and providing insights into the diverse evolutionary process among the morays.
Article
Environmental Sciences
Min-Chen Wang, Fumiya Furukawa, Ching-Wei Wang, Hui-Wen Peng, Ching-Chun Lin, Tzu-Hao Lin, Yung-Che Tseng
Summary: This study explores the transgenerational plasticity in tilapia under global warming scenario and finds that parental cold-experience may affect energy provision during reproduction and early life stages of the offspring. Metabolomics-based profiling reveals potential metabolic traits that help tilapia cope with temperature perturbations. The study also applies feature rescaling and UMAP to visualize metabolic dynamics.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Yi-Chien Lee, Huei-Mien Ke, Yu-Ching Liu, Hsin-Han Lee, Min-Chen Wang, Yung-Che Tseng, Taisei Kikuchi, Isheng Jason Tsai
Summary: Obtaining sufficient genetic material from limited biological sources is a major challenge in biodiversity and genome evolution studies. In this study, we successfully amplified nanograms of DNA and mRNA from individual Caenorhabditis elegans using multiple displacement amplification (MDA) and Smartseq2 techniques. We generated long-read sequences with Oxford Nanopore Technologies (ONT) and achieved high genome coverage. Through genome and transcriptome sequencing of multiple nematode species, we discovered genome diversity and phylogenetic relationships within the phylum Nematoda. This study demonstrates the robustness of MDA and ONT for studying genome diversity.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Ecology
Alex R. Quijada-Rodriguez, Pou-Long Kuan, Po-Hsuan Sung, Mao-Ting Hsu, Garett J. P. Allen, Pung Pung Hwang, Yung-Che Tseng, Dirk Weihrauch
Summary: Anthropogenic CO2 dissolution is leading to chronic acidification in aquatic ecosystems, impacting marine life at both the organism and ecosystem levels. Freshwater acidification, although less studied, can have negative consequences as well. Research on the Chinese mitten crab as a model organism indicates that future freshwater acidification can result in energetic trade-offs, affecting metabolism, calcification, locomotion, and survival rates, ultimately impacting the fitness and mortality of the species.
Article
Cell Biology
Po-Jui Hsu, Horng-Dar Wang, Yung-Che Tseng, Shao-Wei Pan, Bonifasius Putera Sampurna, Yuh-Jyh Jong, Chiou-Hwa Yuh
Summary: This study identified a novel de novo mutation in Tropomyosin 3 (TPM3) associated with congenital myopathy (CM). The mutant gene TPM3(E151G) showed distinct pathogenicity compared to TPM3(E151A) in transgenic fish, leading to impaired muscle function. Treatment with l-carnitine improved muscle endurance by restoring mitochondrial function and gene expression profiles, shedding light on potential therapeutic strategies for TPM3-related CM.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)