Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 79, Issue -, Pages 988-995Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.05.040
Keywords
Silk fibroin; Electrospraying; Microparticle; Cell carrier
Funding
- Marine Biomaterials Research Center grant from the Marine Biotechnology Program - Ministry of Oceans and Fisheries, Korea
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2010-0025378, NRF-2013R1A1A2059239]
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Silk fibroin (SF) is known to be a biocompatible material, and different forms of SF are used for various applications. However, the application of SF in particle form is rarely reported, compared to other forms. In this study, SF microparticles with a diameter of approximately 250 mu m were prepared by the electrospray method, using 1 M LiCl/DMSO as a solvent. The dissolution time of SF in the CaCl2/CH3CH2OH/H2O solution and the concentration of the SF dope solution affected the final morphology of the microparticles. A long dissolution time and a low SF concentration led to the formation of irregular microparticles, but a short dissolution time and a high concentration produced sphere-like microparticles. The shear viscosity of the SF dope solution was the main parameter that affected the morphology of the SF microparticles. Regardless of the dissolution time in the CaCl2/CH3CH2OH/H2O solution and the concentration of the SF dope solution, the shear viscosity of the dope solution must be higher than 0.33 Pa s to produce sphere-like microparticles. Finally, cell adhesion experiments demonstrated that these SF microparticles show potential for use as cell carriers. (C) 2015 Elsevier B.V. All rights reserved.
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