Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Biochemistry & Molecular Biology
Cadenas-Martin Marta, Moratilla Adrian, Fernandez-Delgado Jorge, Arnalich-Montiel Francisco, Maria P. De Miguel
Summary: Corneal disease affects millions worldwide and standard treatment involves transplantation, but there is a shortage. Lamellar endothelial keratoplasty and directed differentiation of adult stem cells show promise in addressing the issue. Efforts are ongoing to find new sources of corneal endothelial cells for transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Szu-Hsien Wu, Yu-Ting Liao, Chi-Han Huang, Yi-Chou Chen, En-Rung Chiang, Jung-Pan Wang
Summary: ADSCs tend to spontaneously differentiate into neuron-like cells during high confluence culture, with enhanced expression of neuronal markers and neurotrophins, while BMSCs do not show this tendency. Additionally, ADSCs do not exhibit spontaneous adipogenic and osteogenic differentiation during this process.
Article
Biochemistry & Molecular Biology
Zahra Eydian, Alaleh Mohammad Ghasemi, Samira Ansari, Ali Naghi Kamali, Maryam Khosravi, Sima Momtaz, Sanaz Riki, Laleh Rafighdoost, Reza Entezari Heravi
Summary: This study demonstrates the differentiation of mesenchymal stem cells (MSCs) from human adipose tissue and bone marrow into functional beta-islet cells. It suggests that adipose stem cells may be a better choice for the treatment of diabetes mellitus due to their higher potency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Cell & Tissue Engineering
Yanrong Yu, Haina Huang, Junsong Ye, Yumei Li, Renjian Xie, Liping Zeng, Yushan Huang, Tai Zeng, Dan Luo, Jianing Zhong, Weijie Peng
Summary: Hepatocyte-like cells (HLCs) derived from mesenchymal stem cells (MSCs) are valuable for drug screening and cell-based regeneration therapy. Differentiating HLCs into 3D spheroids enhances their phenotypes and functions. This study investigates the molecular mechanisms behind MSCs hepatogenic differentiation and identifies the role of p300-mediated H3K56 acetylation in promoting HLCs maturation. The findings suggest that this mechanism could have significant applications in liver regeneration and disease modeling.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Marta Cadenas-Martin, Francisco Arnalich-Montiel, Maria P. De Miguel
Summary: This study developed a protocol for deriving human limbal stem cells (LSCs) from adipose-derived mesenchymal stem cells (ADSCs) that is compatible with the standard carrier human amniotic membrane. The best protocol achieved optimal differentiation efficiency and will benefit patients with LSC deficiency, aiding in the restoration of vision.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ewa Kuca-Warnawin, Magdalena Plebanczyk, Marzena Ciechomska, Marzena Olesinska, Piotr Szczesny, Ewa Kontny
Summary: This study revealed that ASCs derived from SLE and SSc patients exhibit abnormalities in Th cell differentiation at the transcription level, but the functional consequences of this abnormality were not found, as all tested ASCs similarly promoted a shift from Th1 to Th2 direction, increased Th17 formation and IL-17 secretion, and up-regulated classical Tregs generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Saideh Hemati, Ashrafalsadat Hatamian-Zarmi, Raheleh Halabian, Mohsen Ghiasi, Ali Salimi
Summary: The research indicates that SPG can effectively promote osteogenic differentiation of hADMSCs, enhance osteogenic markers and mineralization, and upregulate the expression of bone-specific genes.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Pharmacology & Pharmacy
Annalisa Itro, Maria Consiglia Trotta, Roberta Miranda, Marco Paoletta, Annalisa De Cicco, Caterina Claudia Lepre, Umberto Tarantino, Michele D'Amico, Giuseppe Toro, Alfredo Schiavone Panni
Summary: The aim of this systematic review is to provide a clear overview of the current clinical research progress in the use of adipose-derived mesenchymal stem cells (ASCs) as a therapeutic option for tendinopathies. Seven clinical studies met the inclusion criteria and showed the efficacy and safety of ASCs treatment for tendinopathies. However, there is a lack of clear description of the biomolecular mechanisms underlying the beneficial properties of ASCs.
Article
Biotechnology & Applied Microbiology
Hyun-Ho Yun, Seong-Gon Kim, Se-Il Park, Woori Jo, Kyung-Ku Kang, Eun-Joo Lee, Dong-Kyu Kim, Hoe-Su Jung, Ji-Yoon Son, Jae-Min Park, Hyun-Sook Park, Sunray Lee, Hong-In Shin, Il-Hwa Hong, Kyu-Shik Jeong
Summary: Bone regeneration is a complex process influenced by factors like inflammation, tissue interactions, and progenitor cells. Bone grafting is currently the most effective treatment, but it has drawbacks. Adipose-derived stem cells show promise in accelerating bone regeneration.
BIOENGINEERING-BASEL
(2023)
Review
Multidisciplinary Sciences
Narmadaa Raman, Siti A. M. Imran, Khairul Bariah Ahmad Amin Noordin, Wan Safwani Wan Kamarul Zaman, Fazlina Nordin
Summary: Cardiac muscle cells have the ability to perceive and respond to mechanical strain through mechanotransduction, which can lead to structural and functional changes in the heart. Prolonged or abnormal loading may cause maladaptive remodeling and heart failure. Mesenchymal stem cells offer potential for treating cardiovascular diseases through their immunomodulatory properties and ability to differentiate into cardiomyocytes.
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Article
Cell Biology
Hong Cheng, Yan Huang, Wei Chen, Jifei Che, Taidong Liu, Jing Na, Ruojin Wang, Yubo Fan
Summary: The combinational effect of cyclic strain and electrical stimulation was found to significantly promote the neural differentiation of rat bone marrow-derived mesenchymal stem cells, enhancing the expression of neural markers and upregulating neurotrophic factors and cAMP. In addition, co-stimulation further enhanced calcium influx and phosphorylation of ERK1/2 and AKT in neural differentiated BMSCs, suggesting a synergistic effect of the combination on stem cell neuronal differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
