Journal
AGING-US
Volume 6, Issue 7, Pages 587-601Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.100682
Keywords
Phx1; stationary phase; long-term survival; metabolic flux; pyruvate decarboxylases; ethanol
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Funding
- NRF [NRF-2009- 0079278]
- MEST (Global Frontier Project for Intelligent Synthetic Biology Center [2011-0031960]
- Wellcome Trust
- second-stage BK21 program for Life Sciences at Seoul National University
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In the fission yeast Schizosaccharomyces pombe, the stationary phase-specific transcription factor Phx1 contributes to long-term survival, stress tolerance, and meiosis. We identified Phx1-dependent genes through transcriptome analysis, and further analyzed those related with carbohydrate and thiamine metabolism, whose expression decreased in Delta phx1. Consistent with mRNA changes, the level of thiamine pyrophosphate (TPP) and TPP-utilizing pyruvate decarboxylase activity that converts pyruvate to acetaldehyde were also reduced in the mutant. Therefore, Phx1 appears to shift metabolic flux by diverting pyruvate from the TCA cycle and respiration to ethanol fermentation. Among the four predicted genes for pyruvate decarboxylase, only the Phx1-dependent genes (pdc201(+) and pdc202(+)) contributed to long-term survival as judged by mutation and overexpression studies. These findings indicate that the Phx1-mediated long-term survival is achieved primarily through increasing the synthesis and activity of pyruvate decarboxylase. Consistent with this hypothesis, we observed that Phx1 curtailed respiration when cells entered stationary phase. Introduction of Delta phx1 mutation compromised the long-lived phenotypes of Delta pka1 and Delta sck2 mutants that are devoid of pro-aging kinases of nutrient-signalling pathways, and of the Delta pyp1 mutant with constitutively activated stress-responsive kinase Sty1. Therefore, achievement of long-term viability through both nutrient limitation and anti-stress response appears to be dependent on Phx1.
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