Article
Microbiology
Preeti Sharma, Sumanpreet Kaur, Bhupinder Singh Chadha, Raminderjit Kaur, Manpreet Kaur, Sukhraj Kaur
Summary: Enterocin 12a is a novel bacteriocin with anticancer properties against human cancer cell lines. It selectively inhibits the proliferation of cancer cells in a dose-dependent manner and induces apoptosis-like morphological changes, while having negligible activity towards non-malignant cells. Further evaluation in animal models is recommended to explore its potential as an anticancer agent.
Article
Biotechnology & Applied Microbiology
Samantha Joy D. Valledor, Clarizza May Dioso, Jorge Enrique Vazquez Bucheli, Yu Jin Park, Dong Ho Suh, Eun Sung Jung, Bobae Kim, Wilhelm Heinrich Holzapfel, Svetoslav Dimitrov Todorov
Summary: Enterococcus faecium ST20Kc and ST41Kc isolated from kimchi produce bacteriocins with strong activity against Listeria monocytogenes and various Enterococcus spp. The antimicrobials are proteinaceous and stable, with a bactericidal effect on targeted bacterial strains. Both strains are susceptible to antibiotics and potentially produce additional antimicrobial compounds with metabolomics analysis.
Article
Food Science & Technology
Renpeng Du, Wenxiang Ping, Jingping Ge
Summary: The novel bacteriocin enterocin HDX-2 exhibited high stability and strong bactericidal activity against a variety of bacteria, with mechanisms such as cell lysis, alterations in cell morphology and intracellular activities, and induction of K+ and inorganic phosphate leakage in bacteria.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yunshu Feng, Nan Yin, Zhijiang Zhou, Ye Han
Summary: A composite film named BC-E was prepared using bacterial cellulose (BC) of Gluconacetobacter xylinus and cellfree supernatant (CFS) of Enterococcus faecium TJUQ1, showing significantly improved mechanical properties compared to BC. The BC-E film exhibited higher tensile strength, elongation at break, and water resistance than BC, and effectively reduced the number of Listeria monocytogenes and total mesophilic bacteria in ground meat during storage.
Article
Microbiology
Ge Huang, Yizheng Zhou, Hai Cheng, Tao Lv, Lisi Zheng, Chengbin Li, Yunbo Chen
Summary: Through genomic and transcriptomic analysis, we found differences in carbon metabolism and amino acids between endogenously infected and only-colonized Enterococcus faecium, suggesting metabolic diversity as a strategy for endogenous infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Renata Kwit, Magdalena Zajac, Aleksandra Smialowska-Weglinska, Magdalena Skarzynska, Arkadiusz Bomba, Anna Lalak, Ewelina Skrzypiec, Dominika Wojdat, Weronika Koza, Emilia Mikos-Wojewoda, Paulina Pasim, Milena Skora, Marcin Polak, Jaroslaw Wiacek, Dariusz Wasyl
Summary: This study aimed to assess the prevalence of Enterococcus spp. in wild birds in Poland, determine their antimicrobial susceptibility, and conduct Whole Genome Sequencing (WGS) analysis. The results showed that out of 138 samples from various species of free-living birds, 66.7% tested positive for Enterococcus. Fourteen species were detected, with Enterococcus faecalis being the most common. Resistance to antimicrobial agents was observed in some E. faecalis and E. faecium strains, along with the presence of multidrug resistance phenotype in one E. faecium strain. WGS analysis also revealed variations in virulence gene diversity between E. faecalis and E. faecium strains, as well as the presence of plasmid replicons in both strains.
Article
Genetics & Heredity
Tawsif Ahmed Kazi, Suranjita Mitra, Bidhan Chandra Mukhopadhyay, Sukhendu Mandal, Swadesh Ranjan Biswas
Summary: Enterococcal plasmids, like the novel pSM409 identified in this study, play a critical role in pathogenesis and antibiotic resistance dissemination. The unique sequence structure and distinct DNA-binding domain of pSM409's RepB suggest its potential as a vector for enterococci genetic manipulation. The comparative analysis of pSM409-iteron provides insights into a new classification concept for theta plasmids based on clade-specific relationships.
Article
Food Science & Technology
Yunhe Zhang, Bo Shan, Jiashun Gong, Yongjin Hu
Summary: This study focuses on the regulation of biogenic amine synthesis in Sanchuan ham by quorum sensing. It found that high temperature and low salt conditions promote the production of tyramine by Enterococcus faecium, a strain isolated from Sanchuan ham. It was also discovered that alanyl-leucine acts as a quorum sensing signal molecule, activating the expression of tyrosine decarboxylase and regulating the synthesis of tyramine. These findings provide a theoretical basis for reducing biogenic amine accumulation in ham.
FOOD SCIENCE & NUTRITION
(2022)
Article
Multidisciplinary Sciences
Nashwa Tarek, Ahmed F. Azmy, Ahmed S. Khairalla, Medhat Abdel-Fattah, Ohoud A. Jefri, Mohamed Shaban, Abdelaziz A. A. El-Sayed, Ahmed O. El-Gendy
Summary: This study aimed to isolate and investigate a bacterium from an Egyptian adult's healthy oral cavity, focusing on its probiotic properties, especially its antagonistic activity against oral pathogens. The bacterium NT04 was identified as Enterococcus faecium through 16S rRNA gene sequencing. Genomic analysis confirmed the presence of genes encoding diverse metabolic and probiotic properties in strain NT04. No pathogenicity islands or plasmid insertions were found. The genomic characteristics of strain NT04 support its potentiality as an anti-oral pathogen probiotic candidate.
Article
Microbiology
Yanpeng Xiong, Junwen Chen, Xiang Sun, Guangjian Xu, Peiyu Li, Qiwen Deng, Zhijian Yu, Zhong Chen, Jinxin Zheng
Summary: Telithromycin demonstrated potent in vitro antibacterial activity against clinical enterococci isolates in China, especially strains carrying erythromycin-resistant methylase genes. Subinhibitory concentrations of telithromycin inhibited biofilm formation of Enterococcus strains, and the combination of telithromycin and ampicillin showed enhanced antibiofilm activity against established biofilms. These findings suggest telithromycin's potential as a candidate for enterococcal infection treatment.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Beatriz Rizzo Paschoalini, Karen Vanessa Munive Nunez, Juliana Takahashi Maffei, Helio Langoni, Felipe Freitas Guimaraes, Clarice Gebara, Natylane Eufransino Freitas, Marcos Veiga dos Santos, Carlos Eduardo Fidelis, Roberto Kappes, Monica Correia Goncalves, Nathalia Cristina Cirone Silva
Summary: This study evaluates the antimicrobial resistance and virulence characteristics of Enterococcus spp. isolated from bovine milk, showing high resistance in E. faecium and E. faecalis. The findings highlight the potential risks and the need for control strategies in dairy farms.
Review
Microbiology
Melisa Elsie Kasimin, Suriyani Shamsuddin, Arnold Marshall Molujin, Mohd Khalizan Sabullah, Jualang Azlan Gansau, Roslina Jawan
Summary: Food preservation is a method used to slow down food spoilage and reduce the risk of foodborne illness. Researchers are increasingly focusing on biopreservation techniques, which utilize lactic acid bacteria and their antimicrobial substances to extend shelf life and maintain nutritional value. Enterococcus, a type of lactic acid bacteria, produces a bacteriocin called enterocin that inhibits the growth of pathogenic bacteria. This report highlights the application of Enterococcus in biopreservation of meat and meat-based products, as well as the effects of enzymes, temperature, and pH on the stability of bacteriocin. Industry procedures for meat preservation are also discussed, including their benefits and drawbacks.
Article
Biotechnology & Applied Microbiology
L. D. R. dos Santos, J. P. R. Furlan, I. F. L. Gallo, M. S. Ramos, E. A. Savazzi, E. G. Stehling
Summary: Enterococcus species, including Enterococcus faecium, are part of the microbiota of humans and animals, with strains showing increasing multidrug resistance and carrying virulence genes, posing a threat to nosocomial infections and public health. This study characterized MDR E. faecium isolates from soil and water samples, revealing their resistance to multiple antibiotics and presence of virulence genes. The presence of vanC1-producing E. faecium isolated from soil was reported for the first time in the world, contributing to a better understanding of the environmental sources of antimicrobial resistance in E. faecium isolates carrying virulence genes.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Da Hye Kim, Seul-Ah Kim, Na Gyeong Jo, Jae-Han Bae, Minh Tri Nguyen, Yu Mi Jo, Nam Soo Han
Summary: This study provides evidence that the Enterococcus faecium strain EFEL8600, isolated from Korean soy-meju, exhibits probiotic characteristics including inhibitory activity against Listeria monocytogenes, resilience in gastrointestinal conditions, antioxidant and anti-inflammatory activities, and protection of the intestinal barrier.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Ming Wei, Peng Wang, Tianmeng Li, Qiangyi Wang, Mingze Su, Li Gu, Shuai Wang
Summary: This study evaluated the antimicrobial and antibiofilm activity of essential fatty acids (EFAs) against vancomycin-resistant Enterococcus faecium (VRE-fm) and explored the molecular mechanism of the antibiofilm activity of EFAs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
