Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 26, Issue 1, Pages 174-180Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2015.01.025
Keywords
Callicarpa japonica Thunb.; Acute lung injury; Inducible nitric oxide synthase; Interleukin-6
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Funding
- Ministry of Science, ICT and Future Planning [FGC1011433]
- KRIBB Research Initiative Program of the Republic of Korea [KGM1221521]
- National Research Council of Science & Technology (NST), Republic of Korea [KGM1221521] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Callicarpa japonica Thunb. (CJT) is traditionally used as an herbal remedy for the treatment of inflammatory diseases. This study aimed to investigate the anti-inflammatory response of CJT in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and LPS-induced acute lung injury (ALL) in mice. The C57BL/6 mice were administered 30 mg/kg of CJT by oral gavage for 3 days. LPS is applied to animals by intranasal administration 1 h after final CJT treatment. LPS is applied to animals by intranasal administration 1 h after final CJT treatment LPS was delivered intranasally 1 h after the final CJT treatment In the LPS-stimulated RAW264.7 cells, CJT significantly decreased nitric oxide (NO) and interleukin (IL)-6 in a concentration-dependent manner by reducing inducible NO synthase (iNOS) and IL-6 mRNA levels. In the ALI model, CJT decreased the inflammatory cell count in the bronchoalveolar lavage fluid (BALF) while IL-6 levels were reduced in CJT-treated mice compared with the ALI control mice. CJT also inhibited airway inflammation by reducing iNOS expression in lung tissue. In conclusion, our results indicate that CJT inhibits inflammatory responses in LPS-stimulated RAW264.7 cells and in the LPS-induced ALI model. Therefore, we suggest that CJT has the potential to treat inflammatory diseases such as pneumonia. (c) 2015 Elsevier B.V. All rights reserved.
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