Journal
ADVANCES IN SPACE RESEARCH
Volume 46, Issue 6, Pages 687-700Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.asr.2010.04.012
Keywords
High magnetic gradient environment; Diamagnetic levitation; Simulated weightlessness; Cytoskeleton; Osteoblast
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Funding
- National Natural Science Foundation of China [30970706]
- National High-Tech R&D Program (863) of China [2008AA12A218, 2008AA12A220]
- NPU Foundation for Fundamental Research
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The effects of a high magnetic gradient environment (HMGE) on the cytoskeletal architecture and genes associated with the cytoskeleton in osteoblasts (MC3T3-EI and MG-63 cells) were investigated using confocal microscopy, real-time polymerase chain reaction (PCR) and atomic force microscopy (AFM). The findings showed that, under diamagnetic levitation conditions, the architecture and average height of the cytoskeleton and surface roughness in osteoblasts were dramatically altered. HMGE affects cytoskeleton arrangement and cytoskeleton-associated gene expression. (C) 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.
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