Prediction of naturally-occurring, industrially-induced and total trans fatty acids in butter, dairy spreads and Cheddar cheese using vibrational spectroscopy and multivariate data analysis

This study investigated the use of vibrational spectroscopy [near infrared (NIR), Fourier-transform mid-infrared (FT-MIR), Raman] and multivariate data analysis for (1) quantifying total trans fatty acids (TT), and (2) separately predicting naturally-occurring (NT: i.e., C16:1 t9; C18:1 trans-n, n = 6 ... 9, 10, 11; C18:2 trans) and industrially-induced trans fatty acids (IT = TT - NT) in Irish dairy products, i.e., butter (n = 60), Cheddar cheese (n = 44), and dairy spreads (n = 54). Partial least squares regression models for predicting NT, IT and TT in each type of dairy product were developed using FT-MIR, NIR and Raman spectral data. Models based on NIR, FT-MIR and Raman spectra were used for the prediction of NT and TT content in butter; best prediction performance achieved a coefficient of determination in validation ((RV)-V-2) similar to 0.91-0.95, root mean square error of prediction (RMSEP) similar to 0.07-0.30 for NT; (RV)-V-2 similar to 0.92-0.95, RMSEP similar to 0.23-0.29 for TT. (C) 2015 Elsevier Ltd. All rights reserved.