Journal
ADVANCED SYNTHESIS & CATALYSIS
Volume 354, Issue 18, Pages 3409-3413Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adsc.201200900
Keywords
asymmetric catalysis; biotransformation; organic solvents; reductive amination; -transaminases
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Funding
- European Union [245144 (AmBio-Cas)]
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?-Transaminases, employed as a lyophilised crude cell-free extract, were successfully employed in organic solvent for the asymmetric amination of ketones without the need for immobilisation. Best activity was found for methyl tert-butyl ether (MTBE) at a water activity of 0.6. The ?-transaminases (9 different enzymes) accepted efficiently 2-propylamine as amine donor when used in the solvent, which is not the case when they are used in aqueous solution. The bio-amination in organic solvent showed several advantages such as higher reaction rates (up to 17-fold), general acceptance of 2-propylamine as amine donor, simple work-up procedure (i.e., no basification and extraction required), easy recycling of the catalyst and lack of substrate inhibition. The biocatalysts maintained their excellent stereoselectivity in MTBE allowing the preparation of optically pure amines (ee >99%) with up to >99% conversion.
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