Discovery and Protein Engineering of Biocatalysts for Organic Synthesis

Redesign of the Phosphate Binding Site of L-Rhamnulose- 1-Phosphate Aldolase towards a Dihydroxyacetone Dependent Aldolase

(2011) Xavier Garrabou et al.   ADVANCED SYNTHESIS & CATALYSIS

Enzymatic Asymmetric Synthesis of Enantiomerically Pure Aliphatic, Aromatic and Arylaliphatic Amines with (R)-Selective Amine Transaminases

(2011) Sebastian Schätzle et al.   ADVANCED SYNTHESIS & CATALYSIS

Sitagliptin Manufacture: A Compelling Tale of Green Chemistry, Process Intensification, and Industrial Asymmetric Catalysis

(2011) Aman A. Desai   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome

(2011) Eun Young Yu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering the nucleotide coenzyme specificity and sulfhydryl redox sensitivity of two stress-responsive aldehyde dehydrogenase isoenzymes ofArabidopsis thaliana

(2011) Naim Stiti et al.   BIOCHEMICAL JOURNAL

Substrate specificity of haloalkane dehalogenases

(2011) Tana Koudelakova et al.   BIOCHEMICAL JOURNAL

Structure-Based Redesign of Cofactor Binding in Putrescine Oxidase

(2011) Malgorzata M. Kopacz et al.   BIOCHEMISTRY

Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor

(2011) Li-Juan Wang et al.   BIORESOURCE TECHNOLOGY

A Route to Novel Functional Metalloproteins via Hybrids of Cytochrome P450 and Cytochrome c

(2011) Tianlei Ying et al.   CHEMBIOCHEM

Enhanced Expression and Purification of Fungal Galactose Oxidase in Escherichia coli and Use for Analysis of a Saturation Mutagenesis Library

(2011) Sarah E. Deacon  et al.   CHEMBIOCHEM

A Site-Saturated Mutagenesis Study of Pentaerythritol Tetranitrate Reductase Reveals that Residues 181 and 184 Influence Ligand Binding, Stereochemistry and Reactivity

(2011) Helen S. Toogood et al.   CHEMBIOCHEM

Rational Design of an Aryl-C-Glycoside Catalyst from a Natural Product O-Glycosyltransferase

(2011) Johannes Härle et al.   CHEMISTRY & BIOLOGY

A single-point mutation enables lactate dehydrogenase from Bacillus subtilis to utilize NAD+ and NADP+ as cofactor

(2011) Nina Richter et al.   ENGINEERING IN LIFE SCIENCES

Screening and identification of a novel esterase EstPE from a metagenomic DNA library

(2011) So-Youn Park et al.   JOURNAL OF MICROBIOLOGY

Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

(2011) Olga Khersonsky et al.   JOURNAL OF MOLECULAR BIOLOGY

Conversion of Pyruvate Decarboxylase into an Enantioselective Carboligase with Biosynthetic Potential

(2011) Danilo Meyer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Construction of a Metagenomic Library from Compost and Screening of Cellulase- and Xylanase-positive Clones

(2011) Eun Ju Kwon   Journal of the Korean Society for Applied Biological Chemistry

Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

(2011) Rinkoo D Gupta et al.   Nature Chemical Biology

Stereoselective C–C bond formation catalysed by engineered carboxymethylproline synthases

(2011) Refaat B. Hamed et al.   Nature Chemistry

Changing the substrate specificity of P450cam towards diphenylmethane by semi-rational enzyme engineering

(2011) G. Hoffmann et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Crystallization and preliminary X-ray crystallographic studies of omega-transaminase fromVibrio fluvialisJS17

(2010) Tae-ho Jang et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

A Mutant D-Fructose-6-Phosphate Aldolase (Ala129Ser) with Improved Affinity towards Dihydroxyacetone for the Synthesis of Polyhydroxylated Compounds

(2010) José A. Castillo et al.   ADVANCED SYNTHESIS & CATALYSIS

Highly Stereoselective Synthesis of Substituted Prolyl Peptides Using a Combination of Biocatalytic Desymmetrization and Multicomponent Reactions

(2010) Anass Znabet et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enantioselective Biocatalytic Oxidative Desymmetrization of Substituted Pyrrolidines

(2010) Valentin Köhler et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improving Biocatalyst Performance by Integrating Statistical Methods into Protein Engineering

(2010) M. Brouk et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production

(2010) H. Masukawa et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

(2010) W. Liu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Redesign the α/β Fold to Enhance the Stability of Mannanase Man23 from Bacillus subtilis

(2010) Hai-Yan Zhou et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Engineering of choline oxidase from Arthrobacter nicotianae for potential use as biological bleach in detergents

(2010) Doris Ribitsch et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Identification and characterization of a novel xylanase derived from a rice straw degrading enrichment culture

(2010) Xin-chun Mo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A novel metagenome-derived β-galactosidase: gene cloning, overexpression, purification and characterization

(2010) Kui Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of glycerol dehydrogenase for improved activity towards 1, 3-butanediol

(2010) Hongfang Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of a fungal β-galactosidase to remove product inhibition by galactose

(2010) Xuejun Hu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome mining approach for the discovery of novel cytochrome P450 biocatalysts

(2010) Toshiki Furuya et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cloning and functional characterization of a novel endo-β-1,4-glucanase gene from a soil-derived metagenomic library

(2010) Juan Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A novel cold-active xylanase gene from the environmental DNA of goat rumen contents: Direct cloning, expression and enzyme characterization

(2010) Guozeng Wang et al.   BIORESOURCE TECHNOLOGY

Broadening the cofactor specificity of a thermostable alcohol dehydrogenase using rational protein design introduces novel kinetic transient behavior

(2010) Elliot Campbell et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome mining for the discovery of new nitrilases in filamentous fungi

(2010) Ondřej Kaplan et al.   BIOTECHNOLOGY LETTERS

Rational design of styrene monooxygenase mutants with altered substrate preference

(2010) Abeer Ahmed Qaed et al.   BIOTECHNOLOGY LETTERS

Redesigning the Active Site of Transaldolase TalB from Escherichia coli: New Variants with Improved Affinity towards Nonphosphorylated Substrates

(2010) Sarah Schneider et al.   CHEMBIOCHEM

Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site-Saturated Libraries

(2010) Martyn E. Hulley et al.   CHEMBIOCHEM

Towards Practical Baeyer-Villiger-Monooxygenases: Design of Cyclohexanone Monooxygenase Mutants with Enhanced Oxidative Stability

(2010) Diederik J. Opperman  et al.   CHEMBIOCHEM

LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochrome P450s

(2010) Federico Sabbadin et al.   CHEMBIOCHEM

Recent Developments in the Application of Baeyer-Villiger Monooxygenases as Biocatalysts

(2010) Gonzalo de Gonzalo et al.   CHEMBIOCHEM

The α/β-Hydrolase Fold 3DM Database (ABHDB) as a Tool for Protein Engineering

(2010) Robert Kourist et al.   CHEMBIOCHEM

Natural Diversity to Guide Focused Directed Evolution

(2010) Helge Jochens et al.   CHEMBIOCHEM

Reversed Enantiopreference of an ω-Transaminase by a Single-Point Mutation

(2010) Maria Svedendahl et al.   ChemCatChem

Biocatalytic Reductions and Chemical Versatility of the Old Yellow Enzyme Family of Flavoprotein Oxidoreductases

(2010) Helen S. Toogood et al.   ChemCatChem

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

(2010) Weng Lin Tang et al.   CHEMICAL COMMUNICATIONS

Rational creation of mutant enzyme showing remarkable enhancement of catalytic activity and enantioselectivity toward poor substrates

(2010) Tadashi Ema et al.   CHEMICAL COMMUNICATIONS

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

(2010) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

One pot ‘click’ reactions: tandem enantioselective biocatalytic epoxide ring opening and [3+2] azide alkyne cycloaddition

(2010) Lachlan S. Campbell-Verduyn et al.   CHEMICAL COMMUNICATIONS

Engineering Enzyme Specificity Using Computational Design of a Defined-Sequence Library

(2010) Shaun M. Lippow et al.   CHEMISTRY & BIOLOGY

Metagenomic era for biocatalyst identification

(2010) Lucía Fernández-Arrojo et al.   CURRENT OPINION IN BIOTECHNOLOGY

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering of Enzymes for Selective Catalysis

(2010) Nikhil U. Nair et al.   CURRENT ORGANIC CHEMISTRY

Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation

(2010) Jian Tian et al.   FEBS Journal

Genome mining and biosynthesis of fumitremorgin-type alkaloids in ascomycetes

(2010) Shu-Ming Li   JOURNAL OF ANTIBIOTICS

Identification and characterization of a novel l-amino acid ligase from Photorhabdus luminescens subsp. laumondii TT01

(2010) Kuniki Kino et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Thermostabilization of Bacillus circulans xylanase via computational design of a flexible surface cavity

(2010) Jeong Chan Joo et al.   JOURNAL OF BIOTECHNOLOGY

Engineering of Candida antarctica lipase B for hydrolysis of bulky carboxylic acid esters

(2010) P.B. Juhl et al.   JOURNAL OF BIOTECHNOLOGY

Indication for a New Lipolytic Enzyme Family: Isolation and Characterization of Two Esterases from a Metagenomic Library

(2010) Sally Bayer et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

Manipulating the Stereoselectivity of Limonene Epoxide Hydrolase by Directed Evolution Based on Iterative Saturation Mutagenesis

(2010) Huabao Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed Evolution of an Enantioselective Lipase with Broad Substrate Scope for Hydrolysis of α-Substituted Esters

(2010) Karin Engström et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microbial production of fatty-acid-derived fuels and chemicals from plant biomass

(2010) Eric J. Steen et al.   NATURE

Rational assignment of key motifs for function guides in silico enzyme identification

(2010) Matthias Höhne et al.   Nature Chemical Biology

A new bioinformatics analysis tools framework at EMBL-EBI

(2010) M. Goujon et al.   NUCLEIC ACIDS RESEARCH

Modification of pancreatic lipase properties by directed molecular evolution

(2010) D. Y. Colin et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Improving thermostability of papain through structure-based protein engineering

(2010) Debi Choudhury et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Engineering allosteric regulation into the hinge region of a circularly permuted TEM-1 β-lactamase

(2010) Valéry Mathieu et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Combined use of experimental and computational screens to characterize protein stability

(2010) Nora H. Barakat et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Mutational analysis of phenylalanine ammonia lyase to improve reactions rates for various substrates

(2010) Sebastian Bartsch et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Thermostabilization of an esterase by alignment-guided focussed directed evolution

(2010) Helge Jochens et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Improvement of Bacillus circulans β-amylase activity attained using the ancestral mutation method

(2010) Kan Yamashiro et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18

(2010) Ruipeng Chen et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

A Novel Semi-biosynthetic Route for Artemisinin Production Using Engineered Substrate-Promiscuous P450BM3

(2009) Jeffrey A. Dietrich et al.   ACS Chemical Biology

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Rational Design of Catechol-2, 3-dioxygenase for Improving the Enzyme Characteristics

(2009) Jiashi Wei et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Biocatalytic production of (S)-4-bromo-3-hydroxybutyrate and structurally related chemicals and their applications

(2009) Hiroyuki Asako et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

In vitro evolution of styrene monooxygenase from Pseudomonas putida CA-3 for improved epoxide synthesis

(2009) Lucas J. Gursky et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancing thermostability of a Rhizomucor miehei lipase by engineering a disulfide bond and displaying on the yeast cell surface

(2009) Zhen-lin Han et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The Cytochrome P450 Engineering Database: integration of biochemical properties

(2009) Demet Sirim et al.   BMC BIOCHEMISTRY

Rational Design of a Minimal and Highly Enriched CYP102A1 Mutant Library with Improved Regio-, Stereo- and Chemoselectivity

(2009) Alexander Seifert et al.   CHEMBIOCHEM

Alteration of the Diastereoselectivity of 3-Methylaspartate Ammonia Lyase by Using Structure-Based Mutagenesis

(2009) Hans Raj et al.   CHEMBIOCHEM

Insights into Sequence-Activity Relationships amongst Baeyer-Villiger Monooxygenases as Revealed by the Intragenomic Complement of Enzymes fromRhodococcus jostiiRHA1

(2009) Claudia Szolkowy et al.   CHEMBIOCHEM

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

A Single Point Mutation Reverses the Enantiopreference ofThermoanaerobacter ethanolicusSecondary Alcohol Dehydrogenase

(2009) Musa M. Musa et al.   ChemCatChem

Functional metagenomics for enzyme discovery: challenges to efficient screening

(2009) Taku Uchiyama et al.   CURRENT OPINION IN BIOTECHNOLOGY

Deracemisation of α-Chiral Primary Amines by a One-Pot, Two-Step Cascade Reaction Catalysed by ω-Transaminases

(2009) Dominik Koszelewski et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design

(2009) Oliver Spadiut et al.   FEBS Journal

Engineering thermal stability of l-asparaginase by in vitro directed evolution

(2009) Georgia A. Kotzia et al.   FEBS Journal

Identification of a Novel Self-Sufficient Styrene Monooxygenase from Rhodococcus opacus 1CP

(2009) D. Tischler et al.   JOURNAL OF BACTERIOLOGY

Glyphosate Resistance by Engineering the Flavoenzyme Glycine Oxidase

(2009) Mattia Pedotti et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Reversed Enantioselectivity of Diisopropyl Fluorophosphatase against Organophosphorus Nerve Agents by Rational Design

(2009) Marco Melzer et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Crystal Structures of Cyclohexanone Monooxygenase Reveal Complex Domain Movements and a Sliding Cofactor

(2009) I. Ahmad Mirza et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Laboratory Evolution of Robust and Enantioselective Baeyer−Villiger Monooxygenases for Asymmetric Catalysis

(2009) Manfred T. Reetz et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-Saturation Mutagenesis of Tryptophan 116 of Saccharomyces pastorianus Old Yellow Enzyme Uncovers Stereocomplementary Variants

(2009) Santosh Kumar Padhi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Isolation and Characterization of a GDSL Esterase from the Metagenome of a Marine Sponge-associated Bacteria

(2009) Yoshiko Okamura et al.   MARINE BIOTECHNOLOGY

Finding better protein engineering strategies

(2009) Romas J Kazlauskas et al.   Nature Chemical Biology

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

(2009) Martina Pavlova et al.   Nature Chemical Biology

Protein structure prediction on the Web: a case study using the Phyre server

(2009) Lawrence A Kelley et al.   Nature Protocols

A directed evolution design of a GCG-specific DNA hemimethylase

(2009) Rūta Gerasimaitė et al.   NUCLEIC ACIDS RESEARCH

Computational structure-based redesign of enzyme activity

(2009) C.-Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A family of thermostable fungal cellulases created by structure-guided recombination

(2009) P. Heinzelman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational design-based molecular engineering of the glycosyl hydrolase family 11 B. subtilis XynA endoxylanase improves its acid stability

(2009) T. Belien et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Improving physical realism, stereochemistry, and side-chain accuracy in homology modeling: Four approaches that performed well in CASP8

(2009) Elmar Krieger et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Complete Inversion of Enantioselectivity towards Acetylated Tertiary Alcohols by a Double Mutant of aBacillus Subtilis Esterase

(2008) Sebastian Bartsch et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Self-Sufficient Baeyer–Villiger Monooxygenases: Effective Coenzyme Regeneration for Biooxygenation by Fusion Engineering

(2008) Daniel E. Torres Pazmiño et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli

(2008) S. Atsumi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Protein Engineering of Toluene Monooxygenases for Synthesis of Chiral Sulfoxides

(2008) R. Feingersch et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Directed evolution of a Baeyer–Villiger monooxygenase to enhance enantioselectivity

(2008) Anett Kirschner et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution of aniline dioxygenase for enhanced bioremediation of aromatic amines

(2008) Ee Lui Ang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhancing thermostability of Escherichia coli phytase AppA2 by error-prone PCR

(2008) Moon-Soo Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Knowledge-guided laboratory evolution of protein thermolability

(2008) Manfred T. Reetz et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enzymatic Synthesis of Optically Active Tertiary Alcohols: Expanding the Biocatalysis Toolbox

(2008) Robert Kourist et al.   CHEMBIOCHEM

Directed Evolution of Galactose Oxidase: Generation of Enantioselective Secondary Alcohol Oxidases

(2008) Franck Escalettes et al.   CHEMBIOCHEM

Evolution in Reverse: Engineering a D-Xylose-Specific Xylose Reductase

(2008) Nikhil U. Nair et al.   CHEMBIOCHEM

Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Greatly reduced amino acid alphabets in directed evolution: making the right choice for saturation mutagenesis at homologous enzyme positions

(2008) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Directed Evolution of a Lysosomal Enzyme with Enhanced Activity at Neutral pH by Mammalian Cell-Surface Display

(2008) Kai-Chuan Chen et al.   CHEMISTRY & BIOLOGY

Application of Molecular Modeling for Prediction of Substrate Specificity in Cytochrome P450 1A2 Mutants

(2008) Y. Tu et al.   DRUG METABOLISM AND DISPOSITION

Design and Signaling Mechanism of Light-Regulated Histidine Kinases

(2008) Andreas Möglich et al.   JOURNAL OF MOLECULAR BIOLOGY

Atomistic Insights into the Inhibition of Cysteine Proteases:  First QM/MM Calculations Clarifying the Regiospecificity and the Inhibition Potency of Epoxide- and Aziridine-Based Inhibitors

(2008) Milena Mladenovic et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Most Efficient Cocaine Hydrolase Designed by Virtual Screening of Transition States

(2008) Fang Zheng et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

Directed enzyme evolution via small and effective neutral drift libraries

(2008) Rinkoo D Gupta et al.   NATURE METHODS

The SWISS-MODEL Repository and associated resources

(2008) F. Kiefer et al.   NUCLEIC ACIDS RESEARCH

Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media

(2008) E. Vazquez-Figueroa et al.   PROTEIN ENGINEERING DESIGN & SELECTION

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE