Journal
ADVANCED SYNTHESIS & CATALYSIS
Volume 353, Issue 2-3, Pages 268-274Publisher
WILEY-BLACKWELL
DOI: 10.1002/adsc.201000862
Keywords
dehydrogenation; desaturation; enzymatic oxidation; old yellow enzyme; oxidoreductases; thermophiles
Categories
Funding
- Codexis
- FFG
- Provinces of Styria
- Vienna
- Tyrol
- SFG
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Enzymes from extremophiles have always been of great interest for biotechnology because of their ruggedness against various stress factors. We have isolated, cloned, heterologously expressed and characterized a thermostable old yellow enzyme (OYE) from Geobacillus kaustophilus. In addition to the expected 'enone' reduction, GkOYE also catalyzes the reverse reaction, i.e., the desaturation of C-C bonds adjacent to a carbonyl to give the corresponding alpha,beta-unsaturated ketone. The reaction proceeds at the expense of molecular oxygen without the need for a nicotinamide cofactor and represents an environmentally benign alternative to known chemical dehydrogenation methods.
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