Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 23, Issue 26, Pages 3335-3343Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201202699
Keywords
fluorescent silica nanoparticles; fluorescence microscopy; antibody functionalized silica nanoparticles; flow cytometry
Categories
Funding
- National Science Foundation (NIRT) [CHE 0511219478]
- Maryland Technology Development Corporation
- SD Nanosciences, Inc.
- Maryland NanoCenter
- NispLab.
- Graduate Assistance in Areas of National Need (GAANN) Fellowship
- National Institutes of Health [AI068888, AI09621]
- NSF
Ask authors/readers for more resources
Fluorescent silica nanoparticles (FSNs) are prepared by incorporating dye into a mesoporous silica nanoparticle (MSN) synthesis procedure. FSNs containing sulforhodamine B, hydrophobically modified sulforhodamine B, and Cascade Blue hydrazide are made. The MSN-based FSNs do not leach dye under simulated physiological conditions and have strong, stable fluorescence. FSNs prepared with sulforhodamine B are compared to FSNs prepared with hydrophobically modified sulforhodamine B. The data indicate that FSNs prepared with sulforhodamine B are equally as stable but twice as fluorescent as particles made with hydrophobically modified sulforhodamine B. The fluorescence of a FSN prepared with sulforhodamine B is 10 times more intense than the fluorescence of a 4.5 nm core-shell CdSe/ZnS quantum dot. For diagnostic applications, a method to selectively and covalently bind antibodies to the surface of the FSNs is devised. FSNs that are functionalized with antibodies specific for Neisseria gonorrhoeae specifically bind to Neisseria gonorrhoeae in flow cytometry experiments, thus demonstrating the functionality of the attached antibodies and the potential of MSN-based FSNs to be used in diagnostic applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available