Article
Chemistry, Analytical
Uzeyir Dogan, Ferah Sucularli, Ender Yildirim, Demet Cetin, Zekiye Suludere, Ismail Hakki Boyaci, Ugur Tamer
Summary: Pathogen detection is still a challenging issue in public health, especially in food products. Researchers have developed a method using a capillary-driven microfluidic chip and surface-enhanced Raman scattering measurements for rapid detection of Escherichia coli. This method can detect very low concentrations of E. coli within 60 minutes.
Article
Chemistry, Analytical
Gaozhe Cai, Wenshuai Wu, Shilun Feng, Yuanjie Liu
Summary: An enzyme assay based method in a microfluidic slipchip was proposed for the rapid and label-free detection of E. coli. The method allows for E. coli detection within 5 hours with a low concentration of 8 CFU per chamber, showing great potential in on-site E. coli detection. The steps involve culture, lysis, and enzymatic reaction conducted in a microfluidic slipchip, tailored for fluorescence detection using a commercial plate reader.
Article
Chemistry, Analytical
Wei Wang, Xiaoyu Cai, Qingling Li, Lili Zheng, Xiufeng Yu, Huimin Zhang, Jian Wang
Summary: This study developed an immunoassay method based on a paper chip, which can detect AFP quickly and cost-effectively by immobilizing antibodies on the chip surface. The method showed reliable performance in diagnosing liver diseases by detecting AFP levels in serum samples of normal individuals and patients with liver conditions.
Article
Nanoscience & Nanotechnology
Christian Griesche, Kilian Hoecherl, Antje J. Baeumner
Summary: Laser-induced graphene (LIG) is a desirable electrode material for bio- and chemosensors due to its graphene-like characteristics. The study found that the direct pressure-driven transfer of LIG electrodes onto standard polymer substrates produces highly functional transfer-LIG (tLIG) electrodes which outperformed LIG electrodes in some aspects, especially in more advanced microfluidic channel systems. LIG and tLIG have the potential to change electroanalytical sensing in diagnostic systems with their scalable and easy-to-integrate fabrication process.
ACS APPLIED NANO MATERIALS
(2021)
Article
Multidisciplinary Sciences
Nicola Pellicciotta, Matteo Paoluzzi, Dario Buonomo, Giacomo Frangipane, Luca Angelani, Roberto Di Leonardo
Summary: By modulating bacterial motility with light, researchers have found a way to control the transport direction and speed of microscopic particles by adjusting the active pressure gradients. This discovery provides new insights into the potential use of active pressure for controlled transport of microscopic objects.
NATURE COMMUNICATIONS
(2023)
Article
Thermodynamics
Gautier Guerin, Abdel El Abed
Summary: This paper evaluates a new high throughput temperature measurement method at the micro-scale. The method is non-invasive and utilizes laser-induced fluorescence of highly monodisperse dye-doped microdroplets in microfluidic channels. Real-time temperature sensing is achieved using two thermo-responsive dyes and a single excitation with a 532 nm laser. The ratio of fluorescence intensities from the two dyes shows a-1.4% variation per degree, indicating the method's versatility for different microchannel sizes. The method is also used to evaluate the thermal evolution of microdroplets' inner temperature during cooling, enabling the study of heat exchanges at the microscale.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2023)
Article
Chemistry, Analytical
Sonal Fande, Khairunnisa Amreen, D. Sriram, Sanket Goel
Summary: A microfluidic-based electrochemical device was developed for fast and sensitive detection of Escherichia coli. The device enables incubator-free bacterial culturing in ambient atmosphere and simultaneous monitoring and detection of bacterial growth using electrochemical methods. The device showed high sensitivity and quantification limit, and was successfully used to monitor bacterial growth in real samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Bailey C. Buchanan, Babak Safavinia, Lillian Wu, Jeong-Yeol Yoon
Summary: This study developed a method to detect bacteria on laboratory surfaces using low-cost components and a smartphone, without the need for culturing or staining. The presence of Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus was successfully detected, and the method could also distinguish from tap water, protein, and NaCl solutions.
Review
Biotechnology & Applied Microbiology
Kaimin Wu, Xuliang He, Jinglei Wang, Ting Pan, Ran He, Feizhi Kong, Zhenmin Cao, Feiye Ju, Zhao Huang, Libo Nie
Summary: Microfluidic chip technology is a technology platform that integrates basic operation units such as processing, separation, reaction, and detection into a microchannel chip to achieve low consumption, fast and efficient sample analysis. It has the characteristics of small sample and reagent volume, fast analysis, low cost, automation, portability, high throughput, and good compatibility with other techniques. The future development of microfluidic chips lies in point-of-care testing and high throughput equipment, but there are still challenges in the design and integration of microfluidic chips, as well as the analysis of actual samples.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Optics
Ashkan Ojaghi, Evelyn Kendall Williams, Nischita Kaza, Viswanath Gorti, Hyoann Choi, Jasmine Torey, Tamara Wiley, Brian Turner, Shaquisha Jackson, Sunita Park, Wilbur A. Lam, Francisco E. Robles
Summary: The study presents a simple technique for fast and label-free detection and grading of neutropenia using deep-UV microscopy of blood cells in microfluidic devices. The technique is cost-effective and can be used in low-resource, at-home, or point-of-care settings.
Article
Mechanics
Leonardo Zanini, Cinzia Sada
Summary: This article aims to establish a model to describe all the optical phenomena that occur when light interacts with a moving dispersed phase in a constrained environment, and to quantify the contributions of diffraction, transmission, absorbance, and reflection to the detected intensity. The model has been successfully tested and demonstrates that the intensity changes resulting from the interaction between light and water droplets can be used to obtain information about the droplet characteristics.
Article
Chemistry, Applied
Mantong Zhao, Xiaolong Li, Yule Zhang, Yuwen Wang, Bo Wang, Lulu Zheng, Dawei Zhang, Songlin Zhuang
Summary: An automated microfluidics system was developed for the detection of chloramphenicol (CAP) residues in milk, which had high reliability, low cost, a good detection limit, and recovery rate. This system showed significant potential for point-of-care testing (POCT) of CAP in milk.
Article
Immunology
Po-Cheng Tang, Olle Eriksson, Josefin Sjogren, Nikos Fatsis-Kavalopoulos, Johan Kreuger, Dan I. Andersson
Summary: Biofilms are a crucial mode of growth for bacteria, but how antibiotic resistance emerges and is selected in biofilms is poorly understood. This study developed and validated a microfluidic chip (Brimor) for studying the dynamics of antibiotic-resistant bacteria in biofilms. The chip was found to be easy to use and a relevant model for studying the selection of antibiotic resistance in bacterial biofilms, providing valuable insights into this area of research.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Riccardo Zamboni, Carlos Sebastian-Vicente, Cornelia Denz, Joerg Imbrock
Summary: This study presents an electro-coalescence method for microfluidic droplet surfactant-stabilized emulsion based on light-induced virtual electrodes. By adjusting the exposure time, droplet pairs can be selectively coalesced or the entire emulsion can be dispersed.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Susana P. Costa, Catarina R. F. Caneira, Virginia Chu, Paulo P. Freitas, Joao P. Conde, Carla M. Carvalho
Summary: A novel microfluidic assay combining a bead-based chip and bacteriophage receptor binding proteins (RBPs) was developed for the multiplex detection of Escherichia coli and Pseudomonas aeruginosa in blood. The assay allowed fast and highly specific detection of both pathogens, providing a quantitative assessment of bacterial loads without the need for bacterial pre-enrichment. The device is miniaturized, inexpensive, and has simple fabrication and operation, making it ideal for point-of-care settings.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
