Journal
INSECT SCIENCE
Volume 23, Issue 5, Pages 704-711Publisher
WILEY-BLACKWELL
DOI: 10.1111/1744-7917.12220
Keywords
16S rDNA amplicon pyrosequencing; bacterial diversity; Dialeurolonga malleswaramensis; FISH; mtCO1
Categories
Funding
- National Funds for Basic Strategic and Frontier Application Research in Agriculture (NFBSFARA) of the Indian Council for Agricultural Research (ICAR), Goverment of India
- Council of Scientific and Industrial Research (CSIR), Government of India
Ask authors/readers for more resources
Dialeurolonga malleswaramensis Sundararaj (Hemiptera: Aleyrodidae) is a phytophagous sap sucking insect. It infests Polyalthia longifolia, an important avenue tree of India, effective in alleviating noise pollution and having immensemedicinal importance. Samples of this insect were collected from Polyalthia longifolia. The cytochrome c oxidase subunit I gene (mtCO1) helped in the molecular characterization of the insect. This study reports the bacterial diversity in D. malleswaramensis adults by high throughput 16S rDNA amplicon pyrosequencing. The major genera identified were Portiera and Arsenophonus. Other bacterial genera detected were uncultured alpha proteobacterium, Sphingopyxis and Methylobacterium. We also employed fluorescence in situ hybridization (FISH) in whole mount samples to confirm the presence of dominant endosymbionts Portiera and Arsenophonus to the bacteriocyte of D. malleswaramensis. This study concludes that combining techniques like 16S rDNA amplicon pyrosequencing and FISH reveal both dominant and rare bacteria. The data also predict the evolutionary position of this pest with respect to other whitefly species using a mitochondrial marker.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available