Journal
ACTA TROPICA
Volume 120, Issue 1-2, Pages 40-45Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.actatropica.2011.05.006
Keywords
Malaria; Diagnosis; Real-time PCR; Low parasitemia
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Funding
- Bioneer Corporation, Daejeon, South Korea
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The rapid, accurate diagnosis of Plasmodium spp. is essential for the effective control of malaria, especially in asymptomatic infections. In this study, we developed a sensitive, genus-specific, real-time quantitative PCR assay. It was compared with the microscopic examination of Giemsa-stained blood smears and two different molecular diagnostic techniques: nested PCR and multiplex PCR. For the effective quantitative detection of malaria parasites, all reagents were designed with a lyophilized format in one tube. Plasmodium was detected successfully in all 112 clinically suspected malaria patients, including 32 individuals with low parasitemia (1-100 parasites/mu l). The sensitivity threshold was 0.2 parasites/mu l and no PCR-positive reaction occurred when malaria parasites were not present. This may be a useful method for detecting malaria parasites in endemic areas. (C) 2011 Elsevier B.V. All rights reserved.
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