Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 35, Issue 8, Pages 2393-2406Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-013-1274-1
Keywords
Cucumber; Solexa sequencing; Propamocarb; qRT-PCR; Transcriptome profiling
Categories
Funding
- National Natural Science Foundation of China [31272158]
- 863 Program of National Science and Technology of China [2012AA100105]
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High-throughput tag-sequencing (Tag-seq) from Illumina analysis, which is based on the Solexa Genome Analyzer platform, was applied to analyze the gene expression profiling of propamocarb (PM) treatment and control in cucumber fruit. Approximately 3.6 million complete clean sequence tags at PM treatment or control library were obtained with approximately 0.1 million distinct clean tag sequences. Approximately 41.79-43.15 % of the distinct clean tags were mapped unambiguously to the unigene database, and 32.54-33.46 % of the distinct clean tags were mapped to the cucumber genome database. The profiling analysis of the differentially expressed genes revealed the up-regulation of 546 genes and the down-regulation of 185 genes with PM response. Furthermore, the differentially expressed genes mainly linked to pesticide detoxication, response to stress/stimulus, transporter/signaling, and some important transcription factors. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and reverse transcription polymerase chain reaction (RT-PCR) using 16 genes independently verified the tag-mapped results. The present study reveals the comprehensive mechanisms of PM response in cucumber fruit.
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