Article
Public, Environmental & Occupational Health
Sukanya Panikar, Gunasekaran Shoba, Muthukrishnan Arun, Jesudass Joseph Sahayarayan, A. Usha Raja Nanthini, Arunachalam Chinnathambi, Sulaiman A. Alharbi, Omaima Nasif, Hak-Jae Kim
Summary: This study evaluated the effect of bioactive compounds from Eucalyptus and Corymbia species essential oil on Mpro for potential COVID-19 treatment. Among the compounds studied, eucalyptol showed promising results as an effective therapy option with low toxicity. Further analysis is needed to explore its potential application in medicine.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Review
Biotechnology & Applied Microbiology
Nandakumar Vidya, Krishnagowdu Saravanan, Jayachandran Halka, Kumaresan Kowsalya, Jaganathan Sakthi Yazhini Preetha, Packiaraj Gurusaravanan, Ramalingam Radhakrishnan, A. Usha Raja Nanthini, Muthukrishnan Arun
Summary: Isoflavones are potent secondary metabolites widely distributed among Fabaceae family, with beneficial functions such as lowering cardiovascular diseases and reducing menopausal symptoms. In vitro studies have successfully synthesized value-added metabolites with industrial applications, while plant organ cultures are recommended for synthesizing plant-based pharmaceutically active isoflavones. Biotechnological approaches can promote the yield and production of these metabolites.
PLANT BIOTECHNOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
S. Sandhya, Ramalingam Radhakrishnan, Ramaraj Sathasivam, Muthukrishnan Arun, Gurusaravanan Packiaraj, Sang Un Park
Summary: This study found that the endophytic bacterium FRR2 isolated from the roots of Ficus religiosa has beneficial effects on the growth of Amaranthus campestris, and has tolerance to salinity and heavy metals, possibly through nitrogen fixation, indole acetic acid production, amylase and protease activities. The results suggest that Cellulosimicrobium sp. strain FRR2 could be an effective endophyte to promote the growth of green leafy vegetables.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2021)
Article
Plant Sciences
Ramalingam Radhakrishnan, P. Ajithkumar, Muthukrishnan Arun, Ramaraj Sathasivam, S. Sandhya, Jaehyuk Choi, B. Pradeep, Sang Un Park
Summary: Green leafy vegetables are rich in antioxidants and minerals that prevent food-borne pathogen infections. The endophytic bacterium APL3 was found to significantly enhance the growth of Amaranthus polygonoides L. and increase its antimicrobial potential. Utilization of P. dendritiformis strain APL3 can improve the plants' antimicrobial properties against food-borne pathogens.
PLANT SCIENCE TODAY
(2021)
Article
Biotechnology & Applied Microbiology
Selvam Sathish, Venkatachalam Vasudevan, Sivabalan Karthik, Chandrasekaran Ajithan, Ramamoorthy Siva, Shanthi Pandurengan Parthasarathy, Markandan Manickavasagam
Summary: The use of green synthesized AgNPs enhanced the regeneration frequency of Hybanthus enneaspermus plants and maintained genetic uniformity among the regenerated plants. This micropropagation protocol provides a new approach for targeted gene editing and functional gene analysis in this medicinally significant plant.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Chandrasekaran Ajithan, Venkatachalam Vasudevan, Selvam Sathish, Gadamchetty Pavan, Elangovan Yamini, Markandan Manickavasagam
Summary: An effective protocol for callus-dependent indirect organogenesis in Indian pea cv. Ageta 6 was standardized using cotyledonary node explant. The study highlighted the positive effects of polyamines and plant growth regulators on pea regeneration, and demonstrated that polyamine-assisted regenerated pea plants exhibited improved physiological and antioxidant profiles.
PLANT PHYSIOLOGY REPORTS
(2022)
Review
Plant Sciences
Nandakumar Vidya, Muthukrishnan Arun
Summary: The CRISPR/Cas system is a genome editing technology derived from the adaptive immune system of bacteria, which uses short RNA sequences to guide the Cas enzyme to precise locations within the target genome for gene modification and control. It has been widely used in genome editing, but still has limitations such as the PAM sequence constraint, larger size of the Cas9 gene, and potential off-target effects.
JOURNAL OF PLANT BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jaganathan Sakthi Yazhini Preetha, Muthukrishnan Arun, Nandakumar Vidya, Kumaresan Kowsalya, Jayachandran Halka, Gabrijel Ondrasek
Summary: Arsenic is a highly toxic metalloid that is widely distributed in the Earth's crust, and its contamination caused by human activities is becoming an emerging environmental issue. Sustainable and effective remediation methods, such as microbial remediation and phytoremediation, are needed to prevent and protect against arsenic exposure. Recent advances in genetic modifications and biotechnology have allowed for the development of more efficient transgenic microbes and plants, as well as novel organo-mineral materials for arsenic remediation. This review discusses the latest insights and relevant physiological and molecular mechanisms in arsenic bio-/phytoremediation, providing valuable information for the development of arsenic-tolerant microbes and plants.
Review
Pharmacology & Pharmacy
Selvaraj Perumal, Ramalingam Radhakrishnan, Ramaraj Sathasivam, Muthukrishnan Arun, Jisook Song, Sang Un Park
Summary: Soil and endosymbiotic microorganisms have symbiotic and non-symbiotic relationships with plants, and certain bacteria and fungi have been identified as bio-fertilizers to improve crop productivity. However, there have been limited studies on the beneficial effects of endophytic microbes on drug production in plants. This review focuses on medicinal plants and their associated endophytic bacteria and fungi, exploring their potential in developing drugs against human pathogens and illnesses.
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
(2023)
Article
Genetics & Heredity
Peter Swathik Clarancia, Murugan Naveenarani, Jayanarayanan Ashwin Narayan, Sakthivel Surya Krishna, Prathima Perumal Thirugnanasambandam, Ramanathan Valarmathi, Giriyapur Shivalingamurthy Suresha, Raju Gomathi, Raja Arun Kumar, Markandan Manickavasagam, Ramalingam Jegadeesan, Muthukrishnan Arun, Govindakurup Hemaprabha, Chinnaswamy Appunu
Summary: In this study, 51 NF-Y genes were identified in sugarcane, which are involved in different developmental and stress processes. The expression profiling under drought treatment showed that some NF-Y genes play a role in drought tolerance in a Saccharum hybrid and its drought-tolerant wild relative, Erianthus arundinaceus.
Article
Plant Sciences
Krishnagowdu Saravanan, Nandakumar Vidya, Jayachandran Halka, Ravichandran Priyanka Preethi, Chinnaswamy Appunu, Ramalingam Radhakrishnan, Muthukrishnan Arun
Summary: This study investigated the effects of externally applied stevioside on soybean root growth. Treatment with 24.5μM stevioside significantly increased root length, root numbers, root biomass, shoot length, and shoot biomass. It also enhanced photosynthetic pigments, leaf relative water content, and antioxidant enzymes. However, a higher concentration of stevioside (40.5μM) increased various biochemical parameters such as total polyphenolic content and proline content. Gene expression analysis revealed that stevioside treatment influenced the expression of root growth development-related genes in soybean. Overall, stevioside has the potential to improve morpho-physiological traits, biochemical status, and root development gene expression in soybean.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Krishnagowdu Saravanan, Nandakumar Vidya, Jayachandran Halka, Kumaresan Kowsalya, Chinnaswamy Appunu, Packiaraj Gurusaravanan, Muthukrishnan Arun
Summary: Plant growth and development under salt stress can be improved by exogenous application of onion extract. Onion extract enhances morpho-physiological traits, enzymatic antioxidant activities, and salt tolerance in soybean. It also shows potential as an eco-friendly and cost-effective solution to mitigate abiotic stress in crops.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Krishnagowdu Saravanan, Nandakumar Vidya, Chinnaswamy Appunu, Packiaraj Gurusaravanan, Muthukrishnan Arun
Summary: The study aimed to establish a fast and highly reproducible transformation method for soybean targeting the apical meristem. Modified half-seed explants were treated with different durations of sonication and vacuum infiltration in the presence of Agrobacterium tumefaciens. The optimized protocol achieved a high transformation efficiency of up to 38.0% and could be applied to other Indian soybean cultivars.
Article
Plant Sciences
Murugan Naveenarani, Huskur Kumaraswamy Mahadeva Swamy, Sakthivel Surya Krishna, Channappa Mahadevaiah, Ramanathan Valarmathi, Markandan Manickavasagam, Muthukrishnan Arun, Govindakurup Hemaprabha, Chinnaswamy Appunu
Summary: Phosphorus deficiency has a significant impact on plant growth and development. The use of promoters from phosphate transporter genes can enhance phosphate uptake abilities in genetically modified plants, leading to improved crop yields in phosphate-deficient soils. In this study, the EaPHT1;2 gene from Erianthus arundinaceus was isolated and characterized. The promoter region of EaPHT1;2 showed higher expression than the commonly used CaMV35S promoter. The short promoter sequence of EaPHT1;2 showed significant expression under low-Pi-stress conditions and holds promise for developing stress-tolerant transgenic crops.
Article
Plant Sciences
Sivabalan Karthik, Selvam Sathish, Jesudass Joseph Sahayarayan, Markandan Manickavasagam
Summary: In this study, a novel plant hormone called Meta-topolin (mT) was found to enhance regeneration and genetic transformation efficiency in radish. By adding mT in optimal concentrations to the culture medium, shoot induction, elongation, and rooting abilities of radish were improved. Using Agrobacterium-mediated transformation, mT also increased the efficiency of genetic transformation in radish.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)