Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 35, Issue 3, Pages 829-840Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11738-012-1127-3
Keywords
Cymbidium bicolor; Asymbiotic seed germination; Protocorm; Multiple shoots; Aerides crispum; Mycorrhiza; Fungal co-culture
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Funding
- University Grants Commission (UGC). New Delhi [37-97/2009 (SR)]
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An in vitro plant regeneration protocol was successfully established for Cymbidium bicolor an epiphytic orchid by culturing seeds from green pods. Immature seeds were germinated on four basal media viz., Murashige and Skoog (MS) medium, Knudson C (KC) orchid medium, Knudson C modified Morel (KCM) medium and Lindemann orchid (LO) medium. Seed germination and protocorm development was significantly higher in LO medium (96.6 %) followed by KCM (89 %), MS (77.5 %) and KC (62.7 %) media after 56 days. For multiple shoot induction the protocorms were transferred to B5 medium supplemented with cytokinin. Among the various cytokinins tested, BAP (4.42 mu M) induced maximum (27.59) number of multiple shoots per explant. IBA was effective in inducing healthy roots. Tissue-cultured protocorms and seedlings of C. bicolor were inoculated with AC-01 fungal strain (Moniliopsis sp.) isolated from the mycorrizal roots of an epiphytic orchid Aerides crispum. Mycorrhizal fungi significantly enhanced number of roots, root length and shoot number.
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