Article
Biochemistry & Molecular Biology
Santosh G. Lavhale, Rakesh S. Joshi, Yashwant Kumar, Ashok P. Giri
Summary: Plant 4-coumarate-CoA ligase (4CL) catalyzes the ligation of CoA to cinnamic acid and its derivatives. Okimum kilimandscharicum 4CLs were characterized, showing diversity and potential classification into different classes based on phylogenetic analysis. Two selected Ok4CLs, Ok4CL7 and Ok4CL15, were further characterized in terms of gene expression, enzyme activities, substrate preferences, and potential roles. Further exploration is needed to fully understand the diverse functions of Ok4CLs in O. kilimandscharicum.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Benedikt Wynands, Franziska Kofler, Anka Sieberichs, Nadine da Silva, Nick Wierckx
Summary: Aromatics are valuable chemicals with various important applications, but the majority are currently produced from petroleum with negative environmental impacts. The bio-based synthesis of aromatics using microbial whole-cell catalysis is a promising strategy for a sustainable economy. In this study, we engineered a strain of Pseudomonas taiwanensis for efficient production of 4-coumarate and derived aromatics by optimizing the metabolic pathway. Shake flask batch cultivations achieved high yields of 4-coumarate from glucose and glycerol, and the product spectrum was diversified to include other aromatic compounds.
METABOLIC ENGINEERING
(2023)
Article
Plant Sciences
AiLian Liu, Yue Zhu, YuHao Wang, TianYu Wang, ShuPing Zhao, Kai Feng, LiangJun Li, Peng Wu
Summary: In this study, PAL genes in E. ferox were identified and functionally analyzed. EfPAL1 and EfPAL2 were found to be key genes in flavonoid biosynthesis, and a regulatory network for EfPAL2 was established. These findings provide novel information for understanding the mechanism of flavonoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jihye Seok, Hogyun Seo, Jiyeon Hong, Kyung-Jin Kim
Summary: In this study, the kinetic parameters and biochemical properties of FCHL enzyme from Sphingomonas paucimobilis SYK-6 were investigated, and crystal structures of the enzyme in different forms were determined. The structural changes observed upon substrate binding indicate the substrate promiscuity of SpFCHL.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Shuang Guo, Yue Ma, Yubin Wang, Wenting Zhao, Yanyan Zheng, Pan Wang, Dan Wang, Xiaoyan Zhao
Summary: This study found that storage at 25 degrees C increased the content of bisdemethoxycurcumin in fresh-cut yam, along with enhancing the activities of key enzymes and gene expressions.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Horticulture
Fariborz Habibi, Fabian Guillen, Maria Serrano, Daniel Valero
Summary: This study investigated the effect of postharvest treatment with glycine betaine (GB) on reducing chilling injury (CI) in blood orange cv. Moro during storage at 3 degrees C. Treatment with 30 mM GB significantly reduced CI, as indicated by lower electrolyte leakage, malondialdehyde (MDA), and hydrogen peroxide (H2O2) content compared to the control. Additionally, GB treatment enhanced the activities of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL), and increased proline content, while suppressing polyphenol oxidase (PPO) and peroxidase (POD) activities. Scanning electron microscopy (SEM) images confirmed that 30 mM GB treatment preserved the outer layer of the epidermal cuticle and epidermal wax. These findings suggest that GB treatment may be a promising approach for reducing CI in blood orange fruit at low temperatures.
SCIENTIA HORTICULTURAE
(2022)
Article
Nutrition & Dietetics
Fariborz Habibi, Daniel Valero, Maria Serrano, Fabian Guillen
Summary: The exogenous application of glycine betaine (GB) was found to be effective in maintaining bioactive compounds, antioxidant activity, and quality in Moro blood orange fruit during long-term storage. GB treatments reduced weight and firmness losses, maintained higher concentrations of organic acids and sugars, and enhanced total anthocyanin concentration, total phenolic content, and total antioxidant activity. The most effective treatment was 30 mM GB.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Yuying Wang, Linhui Guo, Yujie Zhao, Xueqing Zhao, Zhaohe Yuan
Summary: 4-Coumarate:CoA ligase (4CL) plays a crucial role in the biosynthesis of phenylpropanoid derivatives and secondary metabolites in pomegranate. A bioinformatics analysis and experimental study were conducted to understand the molecular characteristics and potential biological functions of the 4CL gene family in pomegranate. Twelve Pg4CLs were identified and their expression patterns and possible functions were analyzed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Nicolas Oliveira de Araujo, Mirelle Nayana de Sousa Santos, Fernanda Ferreira de Araujo, Mario Leno Martins Veras, Jean Paulo de Jesus Tello, Rafaela da Silva Arruda, Karen Klotz Fugate, Fernando Luiz Finger
Summary: The sensitivity of different sweet potato cultivars to chilling injury is associated with the activity of their antioxidant systems, with cold-sensitive cultivars showing membrane integrity loss and lipid peroxidation, while cold-tolerant cultivars are able to detoxify hydrogen peroxide through a balanced antioxidant system to alleviate chilling injury symptoms.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Microbiology
Shixin Wang, Zengcai Liu, Xutong Wang, Ruipeng Liu, Li Zou
Summary: This study explores the synthesis of flavonoids in the medicinal mushroom Sanghuangporus baumii through flavonoid-targeted metabolomics and transcriptome analysis. The mushroom synthesized multiple classes of flavonoids on a chemically defined medium, but most of the genes involved in flavonoid biosynthesis were missing. The results suggest that the flavonoid synthesis pathway in S. baumii is different from that in known plants and may involve replacement of missing genes by distantly related ones.
Article
Plant Sciences
Amany H. A. Abeed, Mohammed Ali, Mamdouh A. A. Eissa, Suzan A. A. Tammam
Summary: The application of sewage water for irrigation improves the growth, yield, and quality of Datura innoxia plants compared to well water irrigation. It increases the content of chlorophyll, carotenoids, and non-enzymatic antioxidants in the plants, as well as improves soil quality.
Article
Biochemistry & Molecular Biology
Yannik Brack, Chenghai Sun, Dong Yi, Uwe T. Bornscheuer
Summary: p-Coumaric acid (p-CA) is a key precursor for flavonoid biosynthesis, and tyrosine ammonia lyases (TALs) catalyze the synthesis of p-CA from l-tyrosine. In this study, 875 putative TALs and 46 putative phenylalanine/tyrosine ammonia lyases (PTALs) were analyzed, and 5 TALs and 3 PTALs were characterized and found to exhibit the proposed enzymatic activity. The TAL from Chryseobacterium luteum sp. nov (TAL(clu)) showed the highest affinity and conversion efficiency towards l-tyrosine. Optimization of reaction conditions resulted in p-CA yields of 2.03 g/L after 8 hours by TAL(clu) and 2.35 g/L after 24 hours by TAL from Rivularia sp. PCC 7116 (TAL(rpc)). These TALs are potential candidates for the production of flavonoid precursor p-CA using whole-cell systems.
Article
Agricultural Engineering
Yan Hui Yang, Mu Rong Yang, Jia Yi Chen, Zheng Yang Liu, Yu Xin Zhang, Zhong Yi Zhang, Rui Fang Li
Summary: This study identified and characterized two 4CL genes (Rg4CL1 and Rg4CL2) in Rehmannia glutinosa and revealed their involvement in the biosynthetic pathways of acteoside and flavonoids. Overexpression of Rg4CL1 and Rg4CL2 significantly increased the production of acteoside and flavonoids, respectively.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agriculture, Multidisciplinary
Tamer Ali Sweellum, Deyala M. Naguib
Summary: This study evaluates the role of tomato potato onion intercropping in inducing tomato resistance against Fusarium oxysporum. The intercropping was found to enhance tomato resistance by improving soil enzymes and antifungal enzyme activity, releasing compounds that initiate defense response in tomato plants. This defense response is characterized by elevated antioxidant capacity, antagonistic enzymes against oxidative stress, and improved secondary metabolism.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Biochemistry & Molecular Biology
Xiaoge Gao, Yaping Hu, Zhibiao Xu, Daqing Peng, Qirong Guo
Summary: The PAL gene family in Ginkgo biloba plays a crucial role and exhibits multifunctional characteristics, with specific expression in different tissues and ploidy.
PLANT SIGNALING & BEHAVIOR
(2023)
Article
Plant Sciences
Hanna Bandurska, Justyna Niedziela, Maigorzata Pietrowska-Borek, Katarzyna Nuc, Tamara Chadzinikolau, Dominika Radzikowska
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2017)
Review
Biochemistry & Molecular Biology
Slawomir Borek, Szymon Stefaniak, Jan Sliwinski, Malgorzata Garnczarska, Malgorzata Pietrowska-Borek
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Plant Sciences
Malgorzata Pietrowska-Borek, Anna Wojdyla-Mamon, Jedrzej Dobrogojski, Agnieszka Mlynarska-Cieslak, Marek R. Baranowski, Jakub M. Dabrowski, Joanna Kowalska, Jacek Jemielity, Slawomir Borek, Maria Angeles Pedreno, Andrzej Guranowski
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2020)
Review
Cell Biology
Malgorzata Pietrowska-Borek, Jedrzej Dobrogojski, Ewa Sobieszczuk-Nowicka, Slawomir Borek
Review
Biochemistry & Molecular Biology
Szymon Stefaniak, Lukasz Wojtyla, Malgorzata Pietrowska-Borek, Slawomir Borek
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Plant Sciences
Maria Ulczycka-Walorska, Agnieszka Krzyminska, Hanna Bandurska, Jan Bocianowski
NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
(2020)
Article
Energy & Fuels
Karol Bandurski, Hanna Bandurska, Ewa Kazimierczak-Grygiel, Halina Koczyk
Article
Agronomy
Monika Kozlowska, Hanna Bandurska, Wlodzimierz Bres
Summary: This study aimed to investigate the effects of mine salts on lawn grasses due to road de-icing processes. Results showed that potassium fertilization could alleviate the salinity effects on grasses, improving photosynthesis and lawn growth. It is recommended to use carnallitite instead of halite for de-icing near green areas.
Article
Biochemistry & Molecular Biology
Malgorzata Pietrowska-Borek, Jedrzej Dobrogojski, Anna Maria Wojdyla-Mamon, Joanna Romanowska, Justyna Golebiewska, Slawomir Borek, Koichi Murata, Atsushi Ishihara, Maria Angeles Pedreno, Andrzej Guranowski
Summary: The research demonstrates that specific nucleoside 5'-phosphoramidates can enhance the expression of genes related to the phenylpropanoid pathway in plant cells, leading to the accumulation of related products and providing stress resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Hanna Bandurska, Wlodzimierz Bres, Agnieszka Tomczyk, Malgorzata Zielezinska, Klaudia Borowiak
Summary: This study evaluated the physiological and biochemical responses of Chrysanthemum x grandiflorum 'Palisade White' to long-term salt stress. The results showed that the chrysanthemum had moderate resistance to salt stress and was able to cope with it by maintaining potassium homeostasis and accumulating proline.
Review
Plant Sciences
Hanna Bandurska
Summary: Plants' resistance to stress factors is a complex trait that involves changes at the molecular, metabolic, and physiological levels. Understanding plant coping strategies and their impact on resistance is crucial for agriculture and growers.
Article
Agronomy
Dominika Radzikowska, Hanna Sulewska, Hanna Bandurska, Karolina Ratajczak, Grazyna Szymanska, Przemyslaw Lukasz Kowalczewski, Romana Glowicka-Woloszyn
Summary: Climate change and decreasing rainfall pose challenges to cereal cultivation. However, the interest in ancient wheat varieties, such as spelt, is increasing. This study compares the response to drought stress between spelt and common wheat, and finds that spelt cultivars, particularly 'Franckenkorn' and 'Badengold', exhibit higher resistance to water deficit compared to other cultivars and common wheat.
Article
Green & Sustainable Science & Technology
Hanna Bandurska, Klaudia Borowiak, Malgorzata Zielezinska
Summary: The study observed the response of plants to ozone in different ozone concentration environments, finding that plants exposed to ozone stress increased antioxidant enzyme activity, lipid peroxidation levels, and membrane injury. Although higher ozone concentrations were observed at the forest site, plants in the city site showed a stronger response to ozone.
Article
Environmental Sciences
Hanna Bandurska, Klaudia Borowiak, Malgorzata Zielezinska
ARCHIVES OF ENVIRONMENTAL PROTECTION
(2018)
Article
Biochemistry & Molecular Biology
Jedrzej Dobrogojski, Van Hai Nguyen, Joanna Kowalska, Slawomir Borek, Malgorzata Pietrowska-Borek, Abir U. Igamberdiev
Summary: Dinucleoside polyphosphates are considered novel signalling molecules involved in plant defence mechanisms. This study identified the receptor and signal transduction pathways for these nucleotides, showing that diadenosine tetraphosphate and dicytidine tetraphosphate induce stomatal closure in Arabidopsis thaliana. The plasma membrane purinoreceptor P2K1/DORN1 is essential for the stomata movements induced by diadenosine tetraphosphate, while reactive oxygen species are involved in the signal transduction caused by both nucleotides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)