4.5 Article

Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species

Journal

ACTA PHYSIOLOGIAE PLANTARUM
Volume 31, Issue 3, Pages 649-653

Publisher

SPRINGER HEIDELBERG
DOI: 10.1007/s11738-008-0250-7

Keywords

Encapsulation; Germplasm exchange; Synthetic seeds; Short-term storage; Toothache plant

Categories

Funding

  1. Council of Scientific & Industrial Research (CSIR), New Delhi

Ask authors/readers for more resources

This article demonstrates the plantlet regeneration from alginate-encapsulated shoot tips of Spilanthes acmella. Shoot tip explants excised from in vitro proliferated shoots were encapsulated in calcium alginate beads. The best gel complexation for encapsulation of shoot tips was achieved using 3% sodium alginate and 100 mM calcium chloride. Maximum percent response for the conversion of encapsulated shoot tips into plantlets was obtained on growth regulator-free full-strength liquid MS (Murashige and Skoog, Physiol Plant 15:473-497, 1962) medium. The addition of MS nutrients in alginate matrix was found to have pronounced effect on shoot and root emergence from alginate beads. Encapsulated shoot tips could be stored at low temperature (4A degrees C) up to 60 days. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully. The present synthetic seed technology could be useful in large-scale propagation as well as short-term conservation and germplasm distribution and exchange of Spilanthes acmella.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Biochemistry & Molecular Biology

Somatic Embryogenesis and Plant Regeneration in Sapindus mukorossi Gaertn. from Leaf-Derived Callus Induced with 6-Benzylaminopurine

Reetika Singh, Manoj Kumar Rai, Nishi Kumari

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2015)

Article Agricultural Engineering

Somatic embryogenesis from sepal explants in Sapindus trifoliatus, a plant valuable in herbal soap industry

Pooja Asthana, Manoj K. Rai, Uma Jaiswal

INDUSTRIAL CROPS AND PRODUCTS (2017)

Article Agricultural Engineering

Start codon targeted (SCoT) polymorphism for evaluation of genetic diversity of wild population of Maytenus emarginata

Jatan K. Shekhawat, Manoj K. Rai, N. S. Shekhawat, Vinod Kataria

INDUSTRIAL CROPS AND PRODUCTS (2018)

Article Biochemistry & Molecular Biology

In Vitro Propagation, Encapsulation, and Genetic Fidelity Analysis of Terminalia arjuna: a Cardioprotective Medicinal Tree

Amit K. Gupta, Harish, Manoj K. Rai, Mahendra Phulwaria, Tanvi Agarwal, N. S. Shekhawat

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2014)

Review Biotechnology & Applied Microbiology

Genomic resources in fruit plants: an assessment of current status

Manoj K. Rai, N. S. Shekhawat

CRITICAL REVIEWS IN BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Exploring genetic variability in Prosopis cineraria using two gene targeted CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) polymorphism markers

Jatan K. Shekhawat, Manoj K. Rai, N. S. Shekhawat, Vinod Kataria

MOLECULAR BIOLOGY REPORTS (2018)

Article Plant Sciences

Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT

Udit Sharma, Manoj K. Rai, N. S. Shekhawat, Vinod Kataria

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2019)

Article Biotechnology & Applied Microbiology

In vitro propagation of Farsetia macrantha Blatt. & Hallb.: an endemic and threatened plant of Indian Thar Desert

Dheeraj Choudhary, Manoj K. Rai, N. S. Shekhawat, Vinod Kataria

PLANT CELL TISSUE AND ORGAN CULTURE (2020)

Review Forestry

Genomic and biotechnological interventions in Prosopis cineraria: current status, challenges and opportunities

Manoj K. Rai, Jatan K. Shekhawat, Vinod Kataria, Mahendra Phulwaria, N. S. Shekhawat

Summary: The review discusses the progress in genomics, population genetics, and biotechnology in Prosopis cineraria. The species, important for its tolerance to abiotic stresses, presents potential for gene discovery and crop improvement. However, further research is needed to fully tap into its genetic resources and enhance its adaptability in adverse environmental conditions.

TREES-STRUCTURE AND FUNCTION (2021)

Review Biotechnology & Applied Microbiology

Tissue culture mediated biotechnological interventions in medicinal trees: recent progress

Kavita Arora, Manoj K. Rai, A. K. Sharma

Summary: This review paper presents the recent progress on plant tissue culture-mediated biotechnological advances in medicinal trees, emphasizing different aspects of in vitro propagation, conservation, and production of bioactive compounds of pharmaceutical importance.

PLANT CELL TISSUE AND ORGAN CULTURE (2022)

Review Plant Sciences

Start codon targeted (SCoT) polymorphism marker in plant genome analysis: current status and prospects

Manoj K. K. Rai

Summary: This review provides a comprehensive overview of the start codon targeted (SCoT) polymorphism marker and its applications in various genetic and genomic studies. The SCoT marker is a targeted fingerprinting technique that has gained importance in plant genetics, genomics, and molecular breeding. It targets the highly conserved region flanking the start codon and can distinguish genetic variations in specific genes related to traits. The marker is simple, cost-effective, highly polymorphic, and reproducible, making it valuable for many applications in plant species.

PLANTA (2023)

Article Biotechnology & Applied Microbiology

In vitro selection, regeneration and characterization of NaCl-tolerant plants of Sapindus trifoliatus: an important multipurpose tree

Pooja Asthana, Manoj K. K. Rai, Uma Jaiswal

Summary: The study demonstrates the selection and regeneration of NaCl tolerant plants of Sapindus trifoliatus via somatic embryogenesis. NaCl tolerant callus lines were selected from callus induced from leaf disc explants and were further characterized for their growth, tolerance index, and ion and organic content. Somatic embryos developed from NaCl tolerant callus showed germination on medium with and without NaCl, and the tolerant plants were able to survive in a potting mixture containing NaCl. The physiological and biochemical parameters of the tolerant plants were analyzed, indicating the effectiveness of in vitro selection in developing NaCl tolerant plants of S. trifoliatus.

PLANT CELL TISSUE AND ORGAN CULTURE (2023)

Article Plant Sciences

Synergism of m-topolin with auxin and cytokinin enhanced micropropagation of Maytenus emarginata

Jatan K. Shekhawat, Manoj K. Rai, N. S. Shekhawat, Vinod Kataria

Summary: The synergistic effect of meta-topolin (mT) with auxin and cytokinin effectively stimulates large-scale shoot regeneration in Maytenus emarginata. A combination of 0.5 mg L(-1)mT, 0.25 mg L-1 Kin, and 0.1 mg L-1 IAA was found to be the most effective for shoot proliferation. This study highlights the importance of the interaction between mT, Kin, and IAA in promoting shoot proliferation in medicinal tree species.

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT (2021)

Article Environmental Sciences

Cross species transferability and characterization of microsatellite markers in Prosopis cineraria, a multipurpose tree species of Indian Thar Desert

Manoj K. Rai, Jatan K. Shekhawat, Vinod Kataria, N. S. Shekhawat

ARID LAND RESEARCH AND MANAGEMENT (2017)

Review Biotechnology & Applied Microbiology

Exploring plant tissue culture in Withania somnifera (L.) Dunal: in vitro propagation and secondary metabolite production

Shasmita, Manoj K. Rai, Soumendra K. Naik

CRITICAL REVIEWS IN BIOTECHNOLOGY (2018)

No Data Available