Journal
ACTA PHARMACEUTICA
Volume 60, Issue 1, Pages 1-11Publisher
HRVATSKO FARMACEUTSKO DRUSTOV (HFD)-CROATION PHARMACEUTICAL SOC
DOI: 10.2478/v10007-010-0007-x
Keywords
Ribes biebersteinii Berl. (Grossulariaceae); 2,2-diphenyl-1-picrylhydrazyl (DPPH); cyanidin glycoside; free-radical-scavenger
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The reversed-phase preparative high performance liquid chromatographic purification of the methanol extract of the fruits of Ribes biebersteinii Berl. (Grossulariaceae) afforded five cyanidin glycosides, 3-O-sambubiosyl-5-O-glucosyl cyanidin (1), cyanidin 3-O-sambubioside (2), cyanidin 3-O-glucoside (3), cyanidin 3-O-(2(G)-xylosyl)-rutinoside (4) and cyanidin 3-O-rutinoside (5). They showed considerable free-radical-scavenging properties in the 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay with the RC50 values of 9.29 x 10(-6), 9.33 x 10(-6), 8.31 x 10(-6), 8.96 x 10(-6) and 9.55 x 10(-6) mol L-1, respectively. The structures of these compounds were elucidated by various chemical hydrolyses and spectroscopic means. The total anthocyanin content was 1.9 g per 100 g dried fruits on cyanidin 3-glucoside basis.
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