Journal
ACTA OTO-LARYNGOLOGICA
Volume 128, Issue 11, Pages 1196-1202Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/00016480801918539
Keywords
vestibular hair cells; hydrogen peroxide; reactive oxygen species; cell membrane impairment; viability
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Funding
- Japanese Ministry of Education, Culture, Sports, Science, and Technology [16791027]
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Conclusion. Our findings indicate that oxidative stress induces morphological changes in vestibular hair cells and subsequently leads to cell death after 2.5 h. Objectives. The aim of this study was to confirm the direct effects of oxidative stress on vestibular hair cells. Materials and methods. Vestibular hair cells isolated from guinea pigs were loaded with 1 or 10 mM H2O2, and morphological changes were observed. In addition, in a viability/cytotoxicity assay system, the numbers of dead cells in isolated cristae ampullares were counted 1, 3, and 5 h after loading with H2O2 or artificial perilymph (control). Results. Reactive oxygen, in the form of H2O2, directly affects the cell membrane of isolated vestibular hair cells and causes swelling of the cell body, bleb formation, and shortening of the neck region. Morphological changes occur within 30 min after loading with H2O2, but a significant increase in the number of dead cells is noted only after 3 h.
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