Article
Biochemistry & Molecular Biology
Cody J. Gurski, Bonnie N. Dittel
Summary: Macrophages are important cells in the body that maintain homeostasis and defend against microorganisms. Myeloperoxidase (MPO) can be used as a marker to differentiate between different subsets of monocytes/macrophages with pro-inflammatory and microbicidal activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
P. Vishnyakova, A. Poltavets, E. Karpulevich, A. Maznina, V. Vtorushina, L. Mikhaleva, E. Kananykhina, A. Lokhonina, S. Kovalchuk, A. Makarov, A. Elchaninov, G. Sukhikh, T. Fatkhudinov
Summary: Macrophages play a crucial role in innate immunity and are activated by various factors. This study found that macrophages derived from CD14+ monocytes exhibit higher sensitivity to pro-inflammatory induction, showing increased levels of pro-inflammatory and anti-inflammatory cytokines compared to CD16+-derived macrophages. This data may be valuable for macrophage-based cell therapy applications, providing insights for manipulating donor monocytes for transplantation.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Immunology
Hongxia Li, Zhiqiang Cao, Lili Wang, Chang Liu, Hongkun Lin, Yuhan Tang, Ping Yao
Summary: Cardiovascular diseases are mainly caused by atherosclerosis, and macrophages play a crucial role in its progression. The polarization phenotypes and death pathways of macrophages have an impact on plaque formation and cardiovascular vulnerability.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Arturo Gonzalez de la Aleja, Cristina Herrero, Monica Torres-Torresano, Juan Vladimir de la Rosa, Barbara Alonso, Enrique Capa-Sardon, Ittai B. Muller, Gerrit Jansen, Amaya Puig-Kroeger, Miguel A. Vega, Antonio Castrillo, Angel L. Corbi
Summary: LXR activation limits the anti-inflammatory polarization of human macrophages and promotes the acquisition of an inflammatory profile, which is associated with the expression of specific genes and the release of activin A.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Xiong Liu, Qi Qiao, Xiaonan Li, Xiangjun Ou, Kexin Cui, Boning Niu, Conglian Yang, Li Kong, Zhiping Zhang
Summary: Acute respiratory distress syndrome (ARDS) is a severe respiratory disease with high mortality, but effective therapeutic drugs for clinical practice are lacking. This study constructed an apoptotic neutrophil-mediated drug delivery system for ARDS treatment. The system exhibited significant effects on reducing neutrophil infiltration, inhibiting cytokine storm, and promoting anti-inflammatory macrophage polarization.
Article
Biology
Ausra Mongirdiene, Julius Liobikas
Summary: Chronic heart failure (CHF) is a long-term condition where the heart has difficulty pumping blood enriched in oxygen and required nutrients to the body's tissues. Inflammation is a key factor in promoting CHF, and monocytes, a group of cells in the blood and tissues, play a role in both pro- and anti-inflammatory processes. This review aims to summarize current studies on the function of different monocyte subsets in CHF, particularly in patients with preserved or reduced left ventricle ejection fractions, and their relationship to inflammatory markers.
Article
Physiology
Xinhao Wang, Hongpeng Zhang, Yangyang Ge, Long Cao, Yuan He, Guoyi Sun, Senhao Jia, Airong Ma, Jie Liu, Dan Rong, Wei Guo
Summary: The research revealed that Ang II promotes macrophage M1 polarization and adhesion, leading to upregulation of YAP phosphorylation, inducing EC injury and increasing levels of multiple pro-inflammatory chemokines. Blocking AT1R function or knocking down YAP expression could alleviate the pro-inflammatory effect induced by Ang II, and ARB treatment effectively alleviated these effects, reducing the incidence of AD in mice.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yuri Kim, Ramar Thangam, Jounghyun Yoo, Jeongyun Heo, Jung Yeon Park, Nayeon Kang, Sungkyu Lee, Jiwon Yoon, Kwang Rok Mun, Misun Kang, Sunhong Min, Seong Yeol Kim, Subin Son, Jihwan Kim, Hyunsik Hong, Gunhyu Bae, Kanghyeon Kim, Sanghyeok Lee, Letao Yang, Ja Yeon Lee, Jinjoo Kim, Steve Park, Dong-Hyun Kim, Ki-Bum Lee, Woo Young Jang, Bong Hoon Kim, Ramasamy Paulmurugan, Seung-Woo Cho, Hyun-Cheol Song, Seok Ju Kang, Wujin Sun, Yangzhi Zhu, Junmin Lee, Han-Jun Kim, Ho Seong Jang, Jong Seung Kim, Ali Khademhosseini, Yongju Kim, Sehoon Kim, Heemin Kang
Summary: This study developed a method to induce the swelling and deswelling of microgels using different wavelengths of light, controlling the availability of RGD in the microgels and affecting the polarization of host macrophages, with potential biomedical applications.
ADVANCED MATERIALS
(2022)
Article
Physiology
Peter K. Moore, Kelsey C. Anderson, Shannon A. McManus, Ting -Hui Tu, Emily M. King, Kara J. Mould, Elizabeth F. Redente, Peter M. Henson, William J. Janssen, Alexandra L. McCubbrey
Summary: This study characterized the subtypes and transcriptomic profiles of interstitial macrophages (IMs) in the lung during homeostasis and acute lung inflammation induced by lipopolysaccharide (LPS). The results show that IMs can be divided into distinct subsets based on the expression of folate receptor I3 (FRI3), and during inflammation, there are recruited monocyte-derived IMs with unique gene expression profiles. These findings suggest that IMs have different functions during inflammation.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Amitabh Das, Xiaobei Wang, Jessica Kang, Alyssa Coulter, Amol C. Shetty, Mahesh Bachu, Stephen R. Brooks, Stefania Dell'Orso, Brian L. Foster, Xiaoxuan Fan, Keiko Ozato, Martha J. Somerman, Vivek Thumbigere-Math
Summary: In wild-type mice, Ly6C(hi) and Ly6C(int) monocytes are the primary source of osteoclast formation, with their potential dictated by various factors. In the absence of interferon regulatory factor 8 (IRF8), all three monocyte subsets show higher osteoclastogenic potential. Enhanced NFATc1 nuclear translocation and amplified transcriptomic and epigenetic changes direct increased osteoclastogenesis in IRF8-deficient mice.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Immunology
Xianyan Liu, Jinyu Su, Hui Zhou, Zhiyun Zeng, Zhonghao Li, Zhi Xiao, Ming Zhao
Summary: This study identified COL6A6 as a protective antigen against atherosclerosis and developed a humanized antibody against COL6A6 that can regress atherosclerosis and induce monocyte/macrophage polarization from M1 to M2.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Anindita Bhattacharya, Purnam Ghosh, Arpana Singh, Arnab Ghosh, Arghya Bhowmick, Deepak Kumar Sinha, Abhrajyoti Ghosh, Prosenjit Sen
Summary: The mTOR-dependent classical autophagy is crucial in guiding the differentiation of monocytes into macrophages. Autophagy influences monocyte differentiation through protein degradation and intricately interacts with cell-cycle arrest during the differentiation process.
CELLULAR SIGNALLING
(2021)
Article
Chemistry, Multidisciplinary
Ramar Thangam, Myeong Soo Kim, Gunhyu Bae, Yuri Kim, Nayeon Kang, Sungkyu Lee, Hee Joon Jung, Jinhyeok Jang, Hyojun Choi, Na Li, Minjin Kim, Sangwoo Park, Seong Yeol Kim, Thomas Myeongseok Koo, Hong En Fu, Yoo Sang Jeon, Andreja Ambriovic-Ristov, Jae-Jun Song, Soo Young Kim, Steve Park, Qiang Wei, Changhyun Ko, Ki-Bum Lee, Ramasamy Paulmurugan, Young Keun Kim, Heemin Kang
Summary: Designing materials with remote switchability of decorated nanostructures can promote macrophage adhesion-regulated M2 polarization while suppressing M1 polarization, thereby facilitating tissue-healing responses.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Medicine, Research & Experimental
Dinorah Friedmann-Morvinski, Dolores Hambardzumyan
Summary: Glioblastoma (GBM) is a highly aggressive and common brain tumor in adults. Recent research has revealed the complexity and heterogeneity of GBM, which consists of various neoplastic and non-neoplastic cell types forming the unique tumor microenvironment (TME). Non-neoplastic cells in the TME, particularly myeloid cells infiltrating from the blood circulation, play a crucial role in promoting tumor growth and invasion, and affect the tumor response to therapies. This review focuses on the interactions between neutrophils, monocytes, and neoplastic cells in the GBM TME, discussing the challenges in targeting these cells and potential therapeutic strategies to improve patient outcomes.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Immunology
Victor D. Cuevas, Miriam Simon-Fuentes, Emmanuel Orta-Zavalza, Rafael Samaniego, Paloma Sanchez-Mateos, Maria Escribese, Francisco J. Cimas, Matilde Bustos, Mario Perez-Diego, Alberto Ocana, Angeles Dominguez-Soto, Miguel A. Vega, Angel L. Corbi
Summary: During inflammatory responses, monocytes can differentiate into monocyte-derived macrophages with distinct transcriptional profiles, which are partially regulated by IL-10. This specific gene expression signature may provide potential targets for macrophage-centered therapeutic strategies.
JOURNAL OF INNATE IMMUNITY
(2022)
Review
Immunology
Jamal Hussen, Hans-Joachim Schuberth
Summary: Camels are highly adapted to extreme desert ecosystems and have a strong resistance to pathogens, leading to increased interest in camel immunology. Research focuses on the phenotype and functionality of camel leukocyte subpopulations under different physiological and pathological conditions. Despite progress, gaps remain in understanding camel immune system components such as innate recognition mechanisms and characterization of specific immune cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Jamal Hussen
Summary: The study revealed that incubating camel blood at 41°C in vitro results in a significant increase in apoptotic cells within white blood cell types, with lymphocytes showing higher resistance to heat stress. Additionally, monocytes from camel blood incubated at 41°C for 4 h exhibited a significant decrease in the expression level of MHC class II molecules.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
Francesco Grandoni, Maria Carmela Scata, Alessandra Martucciello, Esterina De Carlo, Giovanna De Matteis, Jamal Hussen
Summary: Monocytes are innate myeloid cells derived from bone marrow, circulating in blood and playing important roles in infection and inflammation. They have functions in ingesting and killing microbes, producing cytokines and chemokines, and presenting antigens to T cells. Studies show that monocyte populations consist of phenotypically and functionally different cell subsets.
Article
Agriculture, Dairy & Animal Science
Jamal Hussen, Mohammed Ali Al-Sukruwah
Summary: This study investigated the effects of a change in animal housing system on the camel immune system. Restricted housing led to significant changes in the composition, phenotype, and function of blood immune cells in dromedary camels.
Article
Multidisciplinary Sciences
Khaled R. Alkharsah, Salma Ali Aljaroodi, Jawad Ur Rahman, Awatif N. Alnafie, Reem Al Dossary, Reem Y. Aljindan, Amani M. Alnimr, Jamal Hussen
Summary: The Saudi population has lower levels of plasma sDPP4 compared to other ethnic groups, which is associated with genetic variants in the DPP4 gene. This may explain the higher number of MERS-CoV cases in Saudi Arabia and their increased susceptibility to the virus.
Article
Veterinary Sciences
Turke Shawaf, Jamal Hussen
Summary: This study describes the cytological analysis of conjunctiva from normal camels and camels with bacterial conjunctivitis. The results showed that camels with conjunctivitis had a higher percentage of basal cells, neutrophils, eosinophils, and macrophages compared to normal camels, while normal camels had an increased percentage of superficial epithelial cells compared to affected camels.
VETERINARY OPHTHALMOLOGY
(2023)
Article
Veterinary Sciences
Gader Abdulaziz Alhafiz, Fatema Hassan Alghatam, Hams Almohammed, Jamal Hussen
Summary: This study aimed to investigate the cellular immune response to subclinical mastitis in the mammary gland of dromedary camels. Through comparing healthy camel milk with milk from camels with subclinical mastitis, it was found that milk from affected animals had higher somatic cell count, increased numbers of CD45+ leukocytes, and an increased fraction of granulocytes with a decreased fraction of macrophages. The decrease in lymphoid cells in the milk from camels with subclinical mastitis was mainly due to the decreased fraction of CD4+ helper T cells.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Abdullah I. A. Al-Mubarak, Jamal Hussen, Mahmoud Kandeel, Anwar A. G. Al-Kubati, Baraa Falemban, Abdullah Skeikh, Maged Gomaa Hemida
Summary: The study investigated the possibility of seroconversion of dromedary camels, sheep, and goats to BVDV along with associated risk factors. Results showed that some dromedary camels, sheep, and goats had seroconverted to BVDV, suggesting their potential roles in the virus transmission cycle. No animals tested positive for the BVDV antigen.
Article
Veterinary Sciences
Turke Shawaf, Hans-Joachim Schuberth, Jamal Hussen
Summary: This study provides the first report on the flow cytometric analysis of immune cell composition in bronchoalveolar lavage fluid (BALF) of dromedary camels. Results show that camels with respiratory diseases have higher total cell count, greater proportion of granulocytes, and lower proportion of macrophages in their BALF compared to healthy camels. Additionally, the percentage of helper T cells and B cells is also higher in diseased camels. The findings suggest an expansion of helper T cells population and an inflammatory macrophage phenotype (M1) in respiratory diseased camels.
BMC VETERINARY RESEARCH
(2022)
Article
Biology
Jamal Hussen, Mayyadah Abdullah Alkuwayti, Baraa Falemban, Mohammed Ali Al-Sukruwah, Sameer M. Alhojaily, Naser Abdallah Al Humam, Salma Al Adwani
Summary: Mastitis is a challenging disease that greatly affects the production and welfare of dairy animals. The study investigated the immunomodulatory effect of selected Toll-like receptor (TLR) ligands on milk immune cells in order to gain a deeper understanding of host-pathogen interactions in the mammary gland. The results may have implications for the development of vaccines against mastitis pathogens.
Article
Veterinary Sciences
Jamal Hussen, Mayyadah Abdullah Alkuwayti, Baraa Falemban, Sameer M. Alhojaily, Salma Al Adwani, El Awad El Hassan, Abdullah I. A. Al-Mubarak
Summary: This study investigated the impact of TLR ligands on the phenotype and function of camel blood neutrophils. Different TLR ligands showed specific modulatory effects on cell size, phagocytosis activity, ROS production, and cell surface antigen expression in camel neutrophils.
VETERINARY SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Abdullah I. A. Al-Mubarak, Jamal Hussen, Mahmoud Kandeel, Anwar A. G. Al-Kubati, Baraa Falemban, Maged Gomaa Hemida
Summary: This study aimed to assess aMPV exposure in backyard chickens and wild birds in Saudi Arabia. Results showed that 74 out of 368 backyard chickens tested positive for aMPV-related antibodies, while none of the tested wild birds seroconverted to aMPV. Further large-scale serological and molecular studies are needed to evaluate the prevalence of aMPV in these birds and characterize the circulating strains of aMPV in this region.
Article
Veterinary Sciences
Abdelazim Ibrahim, Jamal Hussen, Turke Shawaf, Fahad A. Al-Hizab, Matti Kiupel
Summary: A 12-year-old female dromedary camel presented with a large mass on the right eye and progressive weight loss. Postmortem examination revealed multiple nodules in the liver and mesenteric lymph nodes, diagnosed as multicentric T-cell lymphoma. Flow cytometric analysis of collected blood showed a marked increase in the percentage of gamma-delta (workshop cluster 1-positive) lymphoblasts.
VETERINARY RECORD CASE REPORTS
(2023)
Article
Veterinary Sciences
Jamal Hussen, Turke Shawaf, Naser Abdallah Al Humam, Sameer M. Alhojaily, Mohammed Ali Al-Sukruwah, Faisal Almathen, Francesco Grandoni
Summary: Respiratory tract infections have a significant impact on dromedary camels, and this study aimed to establish a flow cytometric protocol for the identification of immune cells in camel lung tissue. The study found that macrophages were the most abundant immune cell population in the lungs, while granulocytes were a minor population. Moreover, the distribution of immune cells varied between different lobes of the camel lung, which could affect the immune response to respiratory pathogens.
VETERINARY SCIENCES
(2022)
Article
Veterinary Sciences
Jamal Hussen, Turke Shawaf, Sameer M. Alhojaily
Summary: This study found that different anticoagulants used for camel leukocytes had varying effects on leukocyte count, immunophenotype, and cell vitality. Lithium heparin resulted in lower numbers of total leukocytes and neutrophils, and higher proportions of necrotic and apoptotic neutrophils and monocytes compared to EDTA. In addition, monocytes and neutrophils in heparin blood had higher expression levels of certain cell surface markers. Therefore, the type of anticoagulant is important to consider when studying camel leukocytes.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Binbin Chang, Zhang Wang, Hui Cheng, Tingyuan Xu, Jieyu Chen, Wan Wu, Yizhi Li, Yong Zhang
Summary: Acacetin can attenuate sepsis-induced ALI by inhibiting the inflammatory response and promoting macrophage polarization. This study is of great significance for the development of new treatments for sepsis-induced ALI.
Review
Biochemistry & Molecular Biology
Nikoleta Bizymi, Andreas M. Matthaiou, Irene Mavroudi, Aristea Batsali, Helen A. Papadaki
Summary: Myeloid-derived suppressor cells (MDSCs) are innate immune cells that have immunomodulatory properties. They interact extensively with other innate or adaptive immune cells and can either enhance or attenuate immune responses depending on the triggering conditions. However, their positive role in host defense mechanisms is rarely discussed in the literature.
