Article
Biochemistry & Molecular Biology
Bryan Le, Kan Zhu, Chelsea Brown, Brian Reid, Amin Cressman, Min Zhao, Fernando A. Fierro
Summary: Corneal wound healing is a complex biological process. Reduced sialylated glycoforms enhance electrotaxis and re-epithelialization, potentially opening new avenues to promote corneal wound healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Kai Furuya, Tao Wu, Ai Orimoto, Eriko Sugano, Hiroshi Tomita, Tohru Kiyono, Takahiro Kurose, Yoshihiro Takai, Tomokazu Fukuda
Summary: The study investigated the expression patterns of immortalized corneal epithelial cells with different genetic modifications, showing that SV40 immortalized cells had a distinct expression pattern from wild-type cells regardless of serum presence, while corneal epithelial cells with combinatorial expression had a pattern closer to that of wild-type cells.
Article
Ophthalmology
Kohei Harada, Hideki Fukuoka, Yuriko Ban, Yulia Aziza, Go Horiguchi, Hidetoshi Tanioka, Shigeru Kinoshita, Masafumi Uematsu, Takashi Kitaoka, Chie Sotozono
Summary: This study evaluated the toxicity of Amphotericin B (AmB) in Optisol (TM)-GS corneal storage media on corneal epithelial cell (CEC) morphology and migration ability. The results showed that AmB concentrations higher than 5μg/ml can be toxic to CECs and inhibit their migration. However, a concentration of 2.5μg/ml of AmB can be considered safe for preserving donor corneal tissue used in corneal epithelial transplantation surgery.
CURRENT EYE RESEARCH
(2022)
Review
Cell Biology
Yue Ruan, Subao Jiang, Aytan Musayeva, Norbert Pfeiffer, Adrian Gericke
Summary: This article discusses the regulation of corneal epithelium regeneration by the limbal stem cell reservoir, the consequences of limbal stem cell deficiency caused by various factors, and the therapeutic options available for treating the condition.
Article
Ophthalmology
Kaveh Mirmoeini, Kiana Tajdaran, Jennifer Zhang, Tessa Gordon, Asim Ali, David R. Kaplan, Konstantin Feinberg, Gregory H. Borschel
Summary: Corneal sensory nerves protect and stimulate the renewal of corneal epithelial cells. Schwann cells play a critical role in this process. The study highlights the importance of limbal Schwann cells in innervation-dependent corneal epithelial renewal.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Shinya Watanabe, Ryuhei Hayashi, Yuzuru Sasamoto, Motokazu Tsujikawa, Bruce R. Ksander, Markus H. Frank, Andrew J. Quantock, Natasha Y. Frank, Kohji Nishida
Summary: A subpopulation of cells in hiPSC-derived corneal epithelial cell sheets, positive for ABCB5, demonstrate remarkable holoclone-forming capabilities that can be suppressed by ABCB5 blockade. These cells first emerge in 2D eye-like organoids around six weeks of differentiation and display corneal epithelial characteristics, including sustained expression of ABCB5. This study highlights the translational potential of ABCB5-enriched, hiPSC-derived corneal epithelial cell sheets for vision recovery in stem cell-deficient human eyes and represents the first report of holoclone-forming stem cells identified in an hiPSC-derived organoid.
Article
Biochemistry & Molecular Biology
Lili Arabuli, Igor A. Iashchishyn, Nina Romanova, Greta Musteikyte, Vytautas Smirnovas, Himanshu Chaudhary, Zeljko M. Svedruzic, Ludmilla A. Morozova-Roche
Summary: The amyloid cascade plays a central role in neurodegenerative disease pathology, with S100A9 protein driving the amyloid-neuroinflammatory cascade. Selected ligands were found to bind to S100A9 with apparent K-d values in the sub-micromolar range. Co-aggregation of S100A9 with these compounds led to morphological changes in amyloid fibrils, thickening them without hindering amyloid formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Koji Kitazawa, Akifumi Matsumoto, Kohsaku Numa, Yasufumi Tomioka, Zhixin A. Zhang, Yohei Yamashita, Chie Sotozono, Judith Campisi
Summary: This study investigated the senescent phenotypes of human corneal and conjunctival epithelial cells and found the characteristics of senescence in these cells. The study also revealed the potential involvement of senescent cells in the pathogenesis of ocular surface diseases.
Article
Ophthalmology
Joo-Hee Park, Kangmin Lee, Choul Yong Park
Summary: This study investigated the effects of magnetic microparticles on the movement of magnet-controlled human corneal endothelial cells (HCECs). The results showed that magnetic microparticles had a certain impact on cell viability and cell movement, and could increase cell density at the appropriate concentration.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2023)
Article
Biology
Yingnan Zhang, Khoi K. Do, Fuhua Wang, Xiaoqin Lu, John Y. Liu, Chi Li, Brian P. Ceresa, Lijun Zhang, Douglas C. Dean, Yongqing Liu
Summary: The cornea is a crucial ocular tissue for protecting the eye. ZEB1, a transcription factor, is found to play a role in corneal epithelial wound healing. Monoallelic deletion of Zeb1 accelerates corneal cell death and inhibits corneal epithelial cell migration. TNF-alpha antagonists may be used to promote corneal wound healing by reducing cell death and inflammation.
COMMUNICATIONS BIOLOGY
(2023)
Article
Ophthalmology
Hui Zhu, Wei Wang, Yongyao Tan, Guanyu Su, Lingjuan Xu, Meng Lin Jiang, Shen Li, Yaa-Jyuhn James Meir, Yunming Wang, Guigang Li, Huamin Zhou
Summary: This study investigates the phenotypic changes of mature corneal epithelial cells (MCECs) when cocultured with limbal niche cells (LNCs) in three-dimensional Matrigel (3D Matrigel) in vitro. The results show that cocultured MCECs express different markers and form holoclones composed of cubic epithelial cells.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Ophthalmology
Wending Yang, Sun Kyong Lee, Ordan J. Lehmann, Zhijian Wu, Suja Hiriyanna, Anand Swaroop, Robert M. Lavker, Han Peng, Tsutomu Kume
Summary: Limbal epithelial stem cells can change from a quiescent mitotic phenotype to a rapidly proliferating cell in response to corneal epithelial wounding. Overexpression of FOXC1 in the limbal epithelium enhances the behavior of stem cells and promotes their transition from quiescence to a highly proliferative status.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Engineering, Biomedical
S-M. Yu, B. Li, F. Amblard, S. Granick, Y-K. Cho
Summary: This study investigated the mechanobiological characteristics of individual cells on a torus structure with different Gaussian curvatures. Cells on the torus surface exhibited topological sensing ability and demonstrated significant adjustments in nuclear area and position depending on the local Gaussian curvature. Spatial heterogeneity of intermediate filament proteins revealed local Gaussian curvature as a key factor of cellular adaptation on curved surfaces.
Article
Ophthalmology
Kirthana Senthil, Haihan Jiao, Laura E. Downie, Holly R. Chinnery
Summary: This study found that exposure to 450 mOsm/L saline for 2 hours induced morphological and phenotypic changes in corneal epithelial dendritic cells, while exposure to 600 mOsm/L saline was toxic to the cells. These results suggest that hyperosmolar conditions activate corneal dendritic cells.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Ophthalmology
Andrew R. Griswold, Hsin-Che Huang, Daniel A. Bachovchin
Summary: The study demonstrates the functional role of NLRP1 inflammasome in human corneal epithelial cells and sheds light on the mechanism through which aberrant inflammasome activation causes corneal damage.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)