Journal
ACTA NEUROLOGICA SCANDINAVICA
Volume 126, Issue -, Pages 90-96Publisher
WILEY
DOI: 10.1111/ane.12029
Keywords
multiple sclerosis; cerebrospinal fluid; biomarkers; proteomics; label-free quantitative mass spectrometry; IgG; vitamin D-binding protein
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Funding
- Kristian Gerhard Jebsen Foundation
- National Program for Research in Functional Genomics (FUGE)
- Research Council of Norway
- Western Norway Regional Healthy Authority
- Leiv Eiriksson Mobility Program
- Meltzer Foundation
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Objectives - The aim of the present study was to identify proteins in cerebrospinal fluid (CSF) with different abundance between patients with relapsing-remitting multiple sclerosis (RRMS) and controls. Such proteins may be diagnostic biomarkers and contribute with novel information about the disease pathogenesis. Materials and methods - Cerebrospinal fluid from patients with RRMS (n = 17) and controls (n = 17) were trypsin digested and analyzed in a label-free fashion using liquid chromatography mass spectrometry. The resulting data were analyzed using SearchGUI, PeptideShaker, and the Progenesis software. Results - Two hundred and ninety-one proteins were identified, of which 32 were significantly differentially abundant between the patients with RRMS and controls (P-value <= 0.05, two or more peptides quantified). Among these were proteins which previously have been linked to MS, including immunoglobulin subunits, vitamin D-binding protein, apolipoprotein D, kallikrein-6, neuronal pentraxin receptor, Dickkopf-related protein 3, and contactin-1. Conclusion - The study provides an overview of differentially abundant proteins between RRMS and controls, and a few of these are further discussed. It should be stressed that a larger verification study is needed to reveal the potential value of these proteins as biomarkers for RRMS and their involvement in the disease pathogenesis.
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