Article
Multidisciplinary Sciences
Jiali Chen, Pai Zhang, Yan Zhao, Jie Zhao, Xiaobo Wu, Ruijia Zhang, Ruitao Cha, Qingxin Yao, Yuan Gao
Summary: This study developed a nitroreductase-instructed supramolecular self-assembly system to enhance the sensitivity of colorectal cancer cells to chemotherapy drugs by agglutinating and eliminating F. nucleatum bacteria. The local introduction of these assemblies efficiently inhibited tumor growth in mice bearing F. nucleatum-infected colorectal cancer. This study broadens the application range of enzyme-instructed supramolecular self-assembly and provides a new approach to treat CRC.
Article
Microbiology
Timmie A. Britton, Chenggang Wu, Yi-Wei Chen, Dana Franklin, Yimin Chen, Martha I. Camacho, Truc T. Luong, Asis Das, Hung Ton-That, Indranil Biswas, Ozlem Yilmaz
Summary: The Gram-negative oral pathobiont Fusobacterium nucleatum plays a role in adverse pregnancy outcomes and colorectal cancer. The highly conserved Rnf complex is key to the metabolic adaptation and virulence of F. nucleatum. Disruption of the Rnf complex leads to defects in coaggregation, ATP production, cell growth, and altered metabolism of amino acids.
Article
Multidisciplinary Sciences
Chenggang Wu, Yi-Wei Chen, Matthew Scheible, Chungyu Chang, Manuel Wittchen, Ju Huck Lee, Truc T. Luong, Bethany L. Tiner, Andreas Tauch, Asis Das, Hung Ton-That
Summary: Fusobacterium nucleatum utilizes the two-component signal transduction system CarRS and environmental lysine to modulate interspecies interactions, virulence, and nutrient acquisition for survival in the adverse environment of oral biofilms and extraoral sites.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Surgery
Yuto Hozaka, Hideyuki Oi, Souichi Satake, Yoshinori Uchino, Yuichi Goto, Tetsuya Idichi, Kiyonori Tanoue, Yoichi Yamasaki, Yota Kawasaki, Yuko Mataki, Hiroshi Kurahara, Akihiro Nakajo, Michiyo Higashi, Akihide Tanimoto, Tsuyoshi Sugiura, Takao Ohtsuka
Summary: This study investigated the association between specific microbiota and the clinicopathologic characteristics of intraductal papillary mucinous neoplasms of the pancreas. They found that the relative abundance of Firmicutes was lower and Proteobacteria and Fusobacteria were higher in the main duct operational taxonomic units compared to branch duct operational taxonomic units. Invasive intraductal papillary mucinous neoplasms had significantly higher relative abundances of Bacteroidetes and Fusobacteria. The relative abundance of the intestinal type was significantly lower in Firmicutes compared to the nonintestinal type.
Article
Immunology
Ruiqi Yang, Tingjun Liu, Chunfeng Pang, Yanling Cai, Zhengmei Lin, Lihong Guo, Xi Wei
Summary: Fusobacterium nucleatum can coaggregate with Streptococcus gordonii in subgingival plaque, inhibiting its adhesion and invasion to human gingival epithelial cells (hGECs) while enhancing the adhesion of S. gordonii. Coaggregation activates the TLR/NF-kappa B and TLR/MAPK signaling pathways, leading to increased secretion of proinflammatory cytokines TNF-alpha and IL-6, and decreased secretion of anti-inflammatory cytokine TGF-beta 1.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Hassan Valadbeigi, Saeed Khoshnood, Babak Negahdari, Abbas Maleki, Medya Mohammadinejat, Mohammad Hossein Haddadi
Summary: This review discusses the role of F. nucleatum in the formation of mixed oral biofilms and its antibiotic resistance. The study highlights the clinical relevance of targeted interactions of F. nucleatum in oral biofilms.
Review
Medicine, Research & Experimental
Slater L. Clay, Diogo Fonseca-Pereira, Wendy S. Garrett
Summary: The microbiota plays an important role in the development of colorectal cancer (CRC). Identifying specific microbial features that impact CRC initiation and progression is crucial. Bacterial factors, such as toxins, contribute to colorectal carcinogenesis. However, further research is needed to understand the host determinants of a toxin's cancer-promoting effects. For bacteria associated with CRC but lacking toxins, defining cancer-relevant virulence factors is challenging. Additionally, gut microbes can have beneficial effects on CRC antitumor immunity through the production of certain metabolites.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Immunology
Qian Li, Hongyan Wang, Lisi Tan, Shuwei Zhang, Li Lin, Xiaolin Tang, Yaping Pan
Summary: The study revealed that the oral pathogen F. nucleatum could synergistically invade pulmonary epithelial cells with P. aeruginosa, leading to increased inflammation and altered cytotoxicity. This coaggregation may contribute to the deterioration of lung function in COPD patients with P. aeruginosa and F. nucleatum coinfection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Yi-Wei Chen, Martha Camacho, Yimin Chen, Aadil H. Bhat, Chungyu Chang, Emily A. Peluso, Chenggang Wu, Asis Das, Hung Ton-That
Summary: In this study, the genetic determinants and physiological functions of hydrogen sulfide (H2S) production in Fusobacterium nucleatum were investigated. The L-methionine gamma-lyase MegL was found to be the major enzyme responsible for H2S production in F. nucleatum, and it significantly contributed to antibiotic susceptibility and bacterial virulence.
Article
Multidisciplinary Sciences
Yoav Charpak-Amikam, Tom Lapidus, Batya Isaacson, Alexandra Duev-Cohen, Tal Levinson, Adi Elbaz, Francesca Levi-Schaffer, Nir Osherov, Gilad Bachrach, Lois L. Hoyer, Maya Korem, Ronen Ben-Ami, Ofer Mandelboim
Summary: This study identifies how Candida albicans evades the immune response by manipulating the immune checkpoint receptor TIGIT. The results suggest that immunotherapy using a TIGIT-blocking antibody can restore anti-Candida immunity and serve as a potential therapeutic tool.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Matthew Scheible, Cuong T. Nguyen, Truc Thanh Luong, Ju Huck Lee, Yi-Wei Chen, Chungyu Chang, Manuel Wittchen, Martha I. Camacho, Bethany L. Tiner, Chenggang Wu, Andreas Tauch, Asis Das, Hung Ton-That
Summary: F. nucleatum is an anaerobic bacterium that colonizes various human tissues. This study uncovered a set of genes in F. nucleatum that play important roles in oxidative stress response and immune evasion, and are critical for its virulence and invasion capabilities.
Article
Food Science & Technology
Takako Taniguchi, Mayuko Ohki, Ayaka Urata, Shoutaro Ohshiro, Elpita Tarigan, Savek Kiatsomphob, Torrung Vetchapitak, Hiroyuki Sato, Naoaki Misawa
Summary: Campylobacter jejuni is the leading cause of bacterial food poisoning worldwide, with chickens being a major reservoir of infection. Treatment and washing of chicken skin significantly reduced the number of C. jejuni attached, suggesting the presence of adhesion factors. Alkaline extracts of the skin surface contained potential binding-related proteins, including chicken serum albumin which specifically bound to C. jejuni adhesins.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Immunology
Dong-Hao Li, Zheng-Peng Li, Yan Zhang, Guan-Zhou Zhou, Rong-Rong Ren, Hui-Jun Zhao, Na-Na Zhang, Jian-Feng Li, Li-Hua Peng, Yun-Sheng Yang
Summary: The presence of Fusobacterium nucleatum (F.nucleatum) and its invasive virulence gene fadA increased in patients with ulcerative colitis, especially in those with severe colitis and pancolitis. Strains of F.nucleatum carrying the virulence gene fadA are suggested to play a role in the pathogenesis of UC.
MICROBIAL PATHOGENESIS
(2021)
Article
Chemistry, Inorganic & Nuclear
Kamila Stokowa-Soltys, Karolina Kierpiec, Robert Wieczorek
Summary: In many cases, human microbiota are associated with cancer progression. It was concluded that the bacterium Fusobacterium nucleatum increases neoplastic changes. Recent studies show that metal complexes with outer membrane protein fragments can promote the formation of reactive oxygen species (ROS), leading to oxidative stress and cell damage. This paper characterizes the formation of copper(ii) complexes with fragments of adhesin YadA from F. nucelatum and demonstrates their ability to produce ROS. Importantly, the free ligands are efficient DNA-cleaving agents.
DALTON TRANSACTIONS
(2022)
Article
Microbiology
Doudou Xiang, Pu-Ting Dong, Lujia Cen, Batbileg Bor, Renate Lux, Wenyuan Shi, Qing Yu, Xuesong He, Tingxi Wu
Summary: This study investigated the potential interaction between F. nucleatum and E. faecalis using phenotype and genetic approaches. The results showed that E. faecalis can physically bind to F. nucleatum and has strong killing ability against it by generating an acidic micro-environment and producing hydrogen peroxide (H2O2). Furthermore, the binding and killing capacities of E. faecalis are necessary to invade and dominate F. nucleatum biofilms.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Asaf Sol, Erion Lipo, Dennise A. de Jesus-Diaz, Connor Murphy, Mildred Devereux, Ralph R. Isberg
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Oncology
Chamutal Gur, Naseem Maalouf, Markus Gerhard, Bernhard B. Singer, Johanna Emgard, Violeta Temper, Tzahi Neuman, Ofer Mandelboim, Gilad Bachrach
Article
Multidisciplinary Sciences
Lishay Parhi, Tamar Alon-Maimon, Asaf Sol, Deborah Nejman, Amjad Shhadeh, Tanya Fainsod-Levi, Olga Yajuk, Batya Isaacson, Jawad Abed, Naseem Maalouf, Aviram Nissan, Judith Sandbank, Einav Yehuda-Shnaidman, Falk Ponath, Joerg Vogel, Ofer Mandelboim, Zvi Granot, Ravid Straussman, Gilad Bachrach
NATURE COMMUNICATIONS
(2020)
Article
Immunology
Jawad Abed, Naseem Maalouf, Abigail L. Manson, Ashlee M. Earl, Lishay Parhi, Johanna E. M. Emgard, Michael Klutstein, Shay Tayeb, Gideon Almogy, Karine A. Atlan, Stella Chaushu, Eran Israeli, Ofer Mandelboim, Wendy S. Garrett, Gilad Bachrach
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Virology
Dan David, Irit Davidson, Sharon Karniely, Nir Edery, Ariela Rosenzweig, Asaf Sol
Summary: During 2019, five carcasses of juvenile Egyptian fruit bats were found to be infected with a Poxvirus, with specific viral genes identified. A new detection method was established and the virus was found to be highly similar to the Israeli Rousettus aegyptiacus Pox Virus.
Article
Cell Biology
Debabrata Biswas, Poornima Ambalavanan, Miriam Ravins, Aparna Anand, Abhinay Sharma, Kimberly Xuan Zhen Lim, Rachel Ying Min Tan, Hwee Ying Lim, Asaf Sol, Gilad Bachrach, Veronique Angeli, Emanuel Hanski
Summary: Research has shown that Group A Streptococcus can resist host immune responses by cleaving the host's antimicrobial peptide LL-37, leading to soft-tissue infections. Furthermore, the activation of host receptors mediated by LL-37 is critical for defense against GAS infections.
Article
Agriculture, Dairy & Animal Science
Gili Schvartz, Sharon Tirosh-Levy, Samantha Barnum, Dan David, Asaf Sol, Nicola Pusterla, Amir Steinman
Summary: Equine coronavirus (ECoV) is an emerging cause of enteric disease in adult horses, with outbreaks reported in the USA, EU, and Japan, as well as sporadic cases in the UK and Saudi Arabia. Infection of ECoV in horses in Israel has never been reported, and the risk of exposure is unknown. The study found that exposure to ECoV in horses in Israel was significantly associated with geographical area.
Article
Immunology
Batya Isaacson, Maya Baron, Rachel Yamin, Gilad Bachrach, Francesca Levi-Schaffer, Zvi Granot, Ofer Mandelboim
Summary: CD300a plays a crucial role in the neutrophil-mediated immune response to UPEC-induced urinary tract infection. Deficiency of CD300a impairs phagocytic abilities of neutrophils, leading to severe exacerbation of infection.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Virology
Dan David, Nick Storm, Waksman Ilan, Asaf Sol
Summary: This study isolated and characterized local BCoV strains in Israel, finding that they belong to different genetic variants and cluster together with other BCoV circulating in the area. These local strains are genetically close to other BCoV strains in the region but distant from the reference enteric strain Mebus.
Article
Immunology
Johanna Galaski, Amjad Shhadeh, Ariana Umana, Christopher C. Yoo, Ludovica Arpinati, Batya Isaacson, Orit Berhani, Bernhard B. Singer, Daniel J. Slade, Gilad Bachrach, Ofer Mandelboim
Summary: F. nucleatum binds and activates CEACAM1 through its surface protein CbpF, leading to inhibition of CD4(+) T cell response and promotion of tumorigenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Amjad Shhadeh, Johanna Galaski, Tamar Alon-Maimon, Jamal Fahoum, Reuven Wiener, Daniel J. Slade, Ofer Mandelboim, Gilad Bachrach
Summary: Recent studies have shown that Fusobacterium nucleatum is present in colorectal, esophageal, and breast cancer, and it can inhibit T and NK cell anti-tumor immunity by activating the human inhibitory receptor CEACAM1. In this study, a recombinant E. coli expressing full-length CbpF was generated, which efficiently binds and activates CEACAM1.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Natalia Golender, Velizar Bumbarov, Anita Kovtunenko, Dan David, Marisol Guini-Rubinstein, Asaf Sol, Martin Beer, Avi Eldar, Kerstin Wernike
Summary: Various viruses, including peribunyaviruses, reoviruses, and pestiviruses, play a significant role in abnormal pregnancies of ruminants in Israel. At least four different BTV serotypes and two BDV strains were identified, showing variation in virus circulation between years. AKAV was the most detected pathogen in small domestic ruminant gestation abnormalities in 2018.
Article
Biochemistry & Molecular Biology
Virve Cavallucci, Ivana Palucci, Marco Fidaleo, Antonella Mercuri, Letizia Masi, Valeria Emoli, Giada Bianchetti, Micol Eleonora Fiori, Gilad Bachrach, Franco Scaldaferri, Giuseppe Maulucci, Giovanni Delogu, Giovambattista Pani
Summary: Fusobacterium nucleatum (Fn) directly targets colorectal cancer stem cells (CR-CSCs), leading to their attachment and activation, which may contribute to the development and treatment of colorectal cancer.
Review
Microbiology
Mwila Kabwe, Stuart Dashper, Gilad Bachrach, Joseph Tucci
Summary: The failure of some cancer treatments has been linked to the tumour microbiota, suggesting that manipulating the microbiota could enhance treatment efficacy. Bacteriophages show potential in targeting oncobacteria prevalent in tumour tissue microbiomes, offering a precise means to improve existing cancer therapies.
FEMS MICROBIOLOGY REVIEWS
(2021)