Article
Immunology
Fatemeh Korpi, Gholamreza Irajian, Fatemeh Forouhi, Taher Mohammadian
Summary: The study shows that a chimeric vaccine including PcrV, FlaA, FlaB, and OprF (PABF) protein can induce a robust immune response, reduce bacterial burden, and improve survival after P. aeruginosa infection. It exhibits broad-spectrum immunity and holds promise as a candidate for treating and controlling P. aeruginosa infections.
MICROBIAL PATHOGENESIS
(2023)
Article
Immunology
Margalida Mateu-Borras, Laura Zamorano, Alex Gonzalez-Alsina, Irina Sanchez-Diener, Antonio Domenech-Sanchez, Antonio Oliver, Sebastian Alberti
Summary: This study demonstrates that the proteases AprA and LasB secreted by Pseudomonas aeruginosa can cleave the complement component C3, but they are not essential for the virulence of bloodstream infections caused by P. aeruginosa.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lin Dai, Lian Lu, Xu Zhang, Juhong Wu, Jinyu Li, Zhonghui Lin
Summary: The Holliday junction branch migrator RuvAB complex is a crucial factor in homologous recombination and DNA damage repair, and can be targeted for the treatment of bacterial pathogens. This study identified small-molecule inhibitors of PaRuvAB, including corilagin, bardoxolone methyl, and 10-(6 '-plastoquinonyl) decyltriphenylphosphonium, which efficiently inhibited the branch migration activity of PaRuvAB and increased the susceptibility of P. aeruginosa to UV-C irradiation.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Virology
Julia E. Egido, Simon O. Dekker, Catherine Toner-Bartelds, Cedric Lood, Suzan H. M. Rooijakkers, Bart W. Bardoel, Pieter-Jan Haas
Summary: This study investigated the effects of serum components, such as the complement system, on the infectivity of different phages. The results showed that some phages were inhibited by serum, while others were not affected. Activation of the classical complement pathway was found to be the main driver of phage inhibition. Further research revealed that phage adsorption was hindered in the presence of active complement. These findings suggest that interactions with the complement system may impact the in vivo activity of therapeutic phages.
Article
Chemistry, Multidisciplinary
Louis M. M. Mouterde, Julien Couvreur, Maxime M. J. Langlait, Fanny Brunois, Florent Allais
Summary: The study identified a potentially new cyclohexanone monooxygenase (CHMO) sequence from the complete genome of the Pseudomonas aeruginosa strain Pa1242, which was confirmed through bioconversion to obtain a key precursor 2H-HBO. The novel sequence exhibited CHMO activity and achieved high productivity and total conversion rates through optimization of culture conditions.
Article
Microbiology
Chenchen Xu, Qiao Cao, Lefu Lan
Summary: Research shows that the periplasmic binding protein GltB plays a crucial role in activating GtrS-GltR in response to glucose in P. aeruginosa and together they regulate the virulence of P. aeruginosa in response to glucose. Furthermore, the activation of GltB is closely related to the virulence of P. aeruginosa.
Article
Oncology
Yang Dong, Wei-Ming Ma, Wen Yang, Lin Hao, Shao-Qi Zhang, Kun Fang, Chun-Hui Hu, Qian-Jin Zhang, Zhen-Duo Shi, Wen-da Zhang, Tao Fan, Tian Xia, Cong-Hui Han
Summary: The study identified differentially expressed genes in ccRCC and found that C3 and FN1 play important roles in the tumorigenesis and progression of ccRCC, potentially serving as biomarkers for predicting patient prognosis.
Article
Immunology
Yingbo Ma, Xueqing Ding, Mingxi Shao, Yichao Qiu, Shengjie Li, Wenjun Cao, Gezhi Xu
Summary: This study investigated the association between serum complement components and AMD. The results showed that higher levels of C1q and lower levels of C3 were associated with increased risk of AMD in female patients. This suggests that the complement classical pathway may be involved in the development of AMD, especially in females.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Dermatology
William D. Jackson, Alessandro Gulino, Liliane Fossati-Jimack, Rocio Castro Seoane, Kunyuan Tian, Katie Best, Joerg Koehl, Beatrice Belmonte, Jessica Strid, Marina Botto
Summary: Research suggests that the complement system plays a role in cSCC, with C3 driving tumorigenesis during chronic skin inflammation independently of downstream generation of C5a or membrane attack complex.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Review
Microbiology
Alex Gonzalez-Alsina, Margalida Mateu-Borras, Antonio Domenech-Sanchez, Sebastian Alberti
Summary: The increasing emergence of multidrug resistant isolates of P. aeruginosa causes major problems in hospitals worldwide. However, the complement system in blood plays a significant role in defense against this pathogen. P. aeruginosa has various strategies to resist complement attack, and understanding these interactions is crucial for the development of drugs to counteract bacterial evasion mechanisms.
Article
Immunology
Xiaoting Wu, Danyu You, Jiong Cui, Liyan Yang, Liyu Lin, Yi Chen, Changsheng Xu, Guili Lian, Jianxin Wan
Summary: C3 plays an important role in inflammation and kidney injury. This study found that ischemia reperfusion injury (IRI) in the kidney leads to increased infiltration of neutrophils and formation of neutrophil extracellular traps (NETs). Inhibiting neutrophil infiltration can reduce kidney injury and inflammation. C3 deficiency can ameliorate acute kidney injury (AKI) by reducing neutrophil infiltration and the formation of NETs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Daniel Yero, Mireia Diaz-Lobo, Lionel Costenaro, Oscar Conchillo-Sole, Adria Mayo, Mario Ferrer-Navarro, Marta Vilaseca, Isidre Gibert, Xavier Daura
Summary: Yero et al. investigate the role of Ttg2D, a Pseudomonas aeruginosa periplasmic protein, in maintaining phospholipid asymmetry between the outer and inner membrane. The study shows that Ttg2D can bind different types of phospholipids and is involved in resistance against certain antibiotics. The structural and functional analysis sheds light on the promiscuous binding capability of Ttg2D and its role in lipid-mediated antibiotic resistance.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Cody James Hastings, Grace Elizabeth Himmler, Arpeet Patel, Claudia Nogueira Hora Marques
Summary: Bacterial persister cells, a metabolically dormant subpopulation tolerant to antimicrobials, play a role in chronic infections and evade host immunity. This study focused on the ability of Pseudomonas aeruginosa persister cells to withstand host innate immunity. The research found that persister cells resist complement-mediated killing and are engulfed at a lower rate. Once engulfed, the cells induce a different macrophage polarization pattern compared to regular vegetative cells. These findings provide further understanding of how persister cells contribute to chronic infections and evade the host immune response.
Article
Endocrinology & Metabolism
Dinko Soic, Jerko Stambuk, Marko Tijardovic, Toma Keser, Gordan Lauc, Tomislav Bulum, Marijana Vucic Lovrencic, Sandra Vuckovic Rebrina, Martina Tomic, Mislav Novokmet, Lea Smircic-Duvnjak, Olga Gornik
Summary: This study investigated the association between C3 N-glycosylation and complications of type 1 diabetes, and found significant changes in C3 N-glycome in severe albuminuria and hypertension accompanying the disease. All C3 glycopeptides except one were associated with HbA1c levels, and one glycoform was associated with non-proliferative diabetic retinopathy. The results suggest that C3 N-glycosylation is a potential novel marker for the progression and severity of type 1 diabetes.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Saulo Relison Tintino, Polrat Wilairatana, Veruska Cintia Alexandrino de Souza, Julia Mariana Assis da Silva, Pedro Silvino Pereira, Cicera Datiane de Morais Oliveira-Tintino, Yedda Maria Lobo Soares de Matos, Joao Tavares Calixto Junior, Valdir de Queiroz Balbino, Jose P. Siqueira-Junior, Irwin Rose Alencar Menezes, Abolghasem Siyadatpanah, Henrique Douglas Melo Coutinho, Tereza Cristina Leal Balbino
Summary: This study evaluated the ability of tannic acid to inhibit the gene expression of the NorA efflux pump in Staphylococcus aureus and assessed its effect on the pump through in silico simulations. The results showed that tannic acid can inhibit the NorA efflux pump by inhibiting gene expression and directly interacting with the NorA protein.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Adam M. Ressler, Mona Abdo, Samantha MaWhinney, Steven C. Johnson, Kristine M. Erlandson
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2019)
Article
Nursing
Mona Abdo, Adam Ressler, Samantha MaWhinney, Catherine Jankowski, Steven C. Johnson, Kristine M. Erlandson
JANAC-JOURNAL OF THE ASSOCIATION OF NURSES IN AIDS CARE
(2021)