Journal
ACTA HISTOCHEMICA ET CYTOCHEMICA
Volume 45, Issue 4, Pages 211-218Publisher
JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY
DOI: 10.1267/ahc.12005
Keywords
brain-derived neurotrophic factor (BDNF); human; saliva; submandibular gland
Categories
Funding
- Japan Society for the Promotion of Science [1989023, 23792157, 20390467, 23390420]
- Grants-in-Aid for Scientific Research [23390420, 24659820, 20390467, 23792157] Funding Source: KAKEN
Ask authors/readers for more resources
Brain-derived neurotrophic factor (BDNF) promotes cell survival and differentiation in the central and peripheral nervous systems. Previously, we reported that BDNF is produced by salivary glands under acute immobilization stress in rats. However, expression of BDNF is poorly understood in humans, although salivary gland localization of BDNF in rodents has been demonstrated. In the present study, we investigated the expression and localization of BDNF in the human submandibular gland (HSG) using reverse transcription-polymerase chain reaction, western blot analysis, in situ hybridization (ISH), immunohistochemistry (IHC), and ELISA. BDNF was consistently localized in HSG serous and ductal cells, as detected by ISH and IHC, with reactivity being stronger in serous cells. In addition, immunoreactivity for BDNF was observed in the saliva matrix of ductal cavities. Western blotting detected one significant immunoreactive 14 kDa band in the HSG and saliva. Immunoreactivities for salivary BDNF measured by ELISA in humans were 40.76 +/- 4.83 pg/mL and 52.64 +/- 8.42 pg/mL, in men and women, respectively. Although salivary BDNF concentrations in females tended to be higher than in males, the concentrations were not significantly different. In conclusion, human salivary BDNF may originate from salivary glands, as the HSG appears to produce BDNF.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available