Article
Cell Biology
Weiqi Zeng, Zhizhao Deng, Yingxin Gao, Guoliang Sun, Xianlong Li, Dongdong Yuan
Summary: Long-term exposure to Ang II significantly enhances the expression and function of Cx43-GJs in HUASMCs, leading to the accumulation of intracellular Ca2+ and the activation of downstream signaling pathways. However, propofol inhibits the function of Cx43-GJs in Ang II-pretreated HUASMCs, resulting in the inhibition of intracellular Ca2+ and its downstream signaling pathways and ultimately excessive relaxation of HUASMCs. This explains why patients with chronic hypertension experience more severe blood pressure fluctuations after propofol induction.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Louis N. Manganas, Irene Dura, Sivan Osenberg, Faith Semerci, Mehmet Tosun, Rachana Mishra, Luke Parkitny, Juan M. Encinas, Mirjana Maletic-Savatic
Summary: This study identified the epitope for the NSC-6 antibody as the signaling protein BASP1, which plays a key role in neurite outgrowth and plasticity. Western blot analysis revealed multiple BASP1 isoforms with varying expression levels, correlating with distinct developmental stages in mouse and human brains. The NSC-6 antibody could be a useful marker for neural stem cells in various developmental stages.
SCIENTIFIC REPORTS
(2021)
Article
Reproductive Biology
Deirdre M. McCarthy, Pradeep G. Bhide
Summary: Research has expanded our understanding of how genetic and environmental factors interact to shape behavioral phenotypes, including environment-induced epigenetic modifications and their potential association with heritable behavioral phenotypes. The molecular basis of phenotypes resulting from environment-induced epigenetic modifications is not well defined, but there is accumulating phenomenological evidence in support of it. Attention has turned to epigenetic modification of germ cells as a possible mechanism of heritability, particularly focusing on the heritable effects of environmental influences on male founders.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biology
Qiang Yue, Otor Al-Khalili, Auriel Moseley, Masaaki Yoshigi, Brandi Michele Wynne, Heping Ma, Douglas C. Eaton
Summary: The interaction between MLP-1, ENaC, and PIP2 was studied, and it was found that PIP2 activates ENaC strongly. MLP-1's positively charged effector domain is required for normal channel activity by controlling the local concentration of PIP2.
Article
Cell Biology
Remi Karadayi, Julie Mazzocco, Laurent Leclere, Benedicte Buteau, Stephane Gregoire, Christine Belloir, Mounzer Koudsi, Pauline Bessard, Jean-Baptiste Bizeau, Elisabeth Dubus, Claire Fenech, Loic Briand, Lionel Bretillon, Alain M. Bron, Xavier Fioramonti, Niyazi Acar
Summary: Plasmalogen deficiency leads to reduced expression of Cx43 in the retina, which is crucial for important functions of Muller cells such as GJIC and cell migration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kristina Rode, Marion Langeheine, Bettina Seeger, Ralph Brehm
Summary: Studies have shown that Cx43 in spermatogonia/spermatids is not essential for successful termination of spermatogenesis, and using a GC-specific Cx43 KO does not affect fertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Won-Young Lee, Heyon Woo Sim, Hyun Jung Park
Summary: Understanding the mechanism of male reproductive system aging and developing anti-aging therapies is vital for preventing male infertility caused by age. Melatonin, a hormone produced by the pineal gland, has antioxidant and anti-apoptotic properties. This study aimed to investigate the effects of melatonin on testicular function in D-galactose-induced aging models. The results showed that melatonin administration suppressed the decline in body weight, sperm vitality, motility, and expression levels of spermatozoa markers. Melatonin also prevented the decrease in the expression of steroidogenic enzyme genes.
Article
Neurosciences
Xinyu Wang, Chuan Jiang, Chunli Lu, Longbing Ma, Yuxin Feng, Shengyu Cui, Qian Li, Kang Li, Xiang Wang, Fengzeng Jian
Summary: In this study, changes in the number and morphology of ependymal cilia in a syringomyelia rat model were investigated. The results showed a decline in cilia during the early stage of the disease, leading to disrupted intercellular communication. In the later stage, cilia were further reduced due to shear stress exposure, causing a potential mechanism of ependymal cilia decline in syringomyelia development.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Biochemistry & Molecular Biology
Daniel Rodjakovic, Lilian Salm, Guido Beldi
Summary: Recent studies have shown that Connexin-43 plays important roles in macrophages, including migration, antigen-presentation, intercellular communication, electrochemical support, and immunomodulation. Dysregulation of Cx43 has been described in diseases such as inflammatory bowel disease, tumors, and HIV. However, its involvement in other macrophage functions and inflammatory processes requires further research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Xiaoping Li, Yunshuo Xiao, Xiaoqi Wang, Ruihao Huang, Rui Wang, Yi Deng, Jun Rao, Qiangguo Gao, Shijie Yang, Xi Zhang
Summary: In this study, it was found that Cx43 deficiency exists in CML patients, and its expression is negatively correlated with HIF-1 alpha. Further experiments revealed that overexpression of Cx43 in BMSCs can increase apoptosis rate and block cell cycle in CML cells, while the opposite was observed in the absence of Cx43. Cx43 mediates gap junction intercellular communication (GJIC) through direct contact, and Ca2+ is the key factor mediating the downstream apoptotic pathway. Animal experiments confirmed the findings in vitro, suggesting that enhancing Cx43 expression and GJIC function in the HM may be a novel strategy to reverse drug resistance and promote IM efficacy.
CHINESE MEDICAL JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Yan-Jun Zong, Xiao-Zhou Liu, Lei Tu, Yu Sun
Summary: This review discusses the contribution of the connexin gene family to hearing loss, focusing on the GJB2, GJB6, and GJA1 genes. Mutations in these genes can lead to comprehensive or non-comprehensive genetic deafness in newborns. The precise regulation of connexin biosynthesis, structural composition, and degradation is crucial for proper functioning of gap junctions. This review provides insights into the etiological principles of connexin mutations and offers potential therapeutic strategies for hereditary deafness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Yuxi Li, Hsun-Ming Chang, Yu-Wen Sung, Hua Zhu, Peter C. K. Leung, Ying-Pu Sun
Summary: In this study, it was found that the phosphorylation of connexin 43 (Cx43) is mediated by protein kinase C (PKC) signaling pathway, leading to a decline in gap junction intercellular communication (GJIC) and subsequent effects on oocyte meiotic resumption. This finding is crucial for understanding the molecular mechanisms of oocyte meiotic resumption.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Multidisciplinary Sciences
Chen Ni, Xiaohan Lou, Xiaohan Yao, Linlin Wang, Jiajia Wan, Xixi Duan, Jialu Liang, Kaili Zhang, Yuanyuan Yang, Li Zhang, Chanjun Sun, Zhenzhen Li, Ming Wang, Linyu Zhu, Dekang Lv, Zhihai Qin
Summary: This study reveals a mechanism by which a subset of zinc-transporter positive cancer-associated fibroblasts directly interacts with lung cancer cells through gap junctions, promoting chemoresistance in tumors. This finding provides insights into the role of fibroblast-tumor cell interactions in lung cancer chemo-resistance.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Yasunari Seita, Keren Cheng, John R. McCarrey, Nomesh Yadu, Ian H. Cheeseman, Alec Bagwell, Corinna N. Ross, Isamar Santana Toro, Li-hua Yen, Sean Vargas, Christopher S. Navara, Brian P. Hermann, Kotaro Sasaki
Summary: This study successfully established a method for culturing mid-gestation mouse iPSCs on a feeder layer, which can further differentiate into cells similar to primordial germ cells (PGCLCs). Furthermore, these cells can acquire an early developmental germ cell phenotype when co-cultured with mouse testicular somatic cells.
Article
Pharmacology & Pharmacy
Keerthana Karunakar Poojary, Guruprasad Nayak, Ashna Vasani, Sandhya Kumari, Reyon Dcunha, Jyolsna Ponnaratta Kunhiraman, Divya Gopalan, Rajat Radhakrishna Rao, Srinivas Mutalik, Sneha Guruprasad Kalthur, M. S. Murari, Shamprasad Varija Raghu, Satish Kumar Adiga, Guruprasad Kalthur
Summary: The study found that curcumin nanocrystals have protective effects on mitigating cyclophosphamide-induced testicular toxicity.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Cell Biology
Abril Alondra Barrientos-Bonilla, Paola Belem Pensado-Guevara, Abraham Puga-Olguin, Rasajna Nadella, Aurora del Carmen Sanchez-Garcia, Laura Mireya Zavala-Flores, Arnulfo Villanueva-Olivo, Iliana Tamara Cibrian-Llanderal, Maria de Jesus Rovirosa-Hernandez, Daniel Hernandez-Baltazar
Summary: This study evaluated the impact of BrdU on liver function and found that multiple injections of BrdU did not induce hepatocellular damage.
Article
Cell Biology
Pan Zhang, Qun Feng, Wenxiao Chen, Xizhuang Bai
Summary: It was found that catalpol antagonizes LPS-mediated inflammation and suppressive osteoblast differentiation by inhibiting inflammation and promoting osteoblast differentiation. This finding contributes to a better understanding of the pathogenesis of osteoporosis.