Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 68, Issue -, Pages 1089-1093Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S174430911203062X
Keywords
succinyl-diaminopimelate desuccinylase; DapE; Rv1202; Mycobacterium tuberculosis
Funding
- BMBF/PTJ [BIO/0312992 A]
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Succinyl-diaminopimelate desuccinylase from Mycobacterium tuberculosis (DapE, Rv1202) has been cloned, heterologously expressed in Escherichia coli and purified using standard chromatographic techniques. Diffraction-quality crystals were obtained at acidic pH from ammonium sulfate and PEG and diffraction data were collected from two crystals to resolutions of 2.40 and 2.58 angstrom, respectively. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 79.7, b = 76.0, c = 82.9 angstrom,beta = 119 degrees. The most probable content of the asymmetric unit was two molecules of DapE, which would correspond to a solvent content of 56%. Both examined crystals turned out to be pseudo-merohedrally twinned, with twin operator -h, -k, h + l and twin fractions of approximately 0.46 and 0.16, respectively.
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